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Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.

Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini LR, Fanale D, Margarese N, Santini D, Bazan V, Russo A - J. Cell. Mol. Med. (2012)

Bottom Line: Microarrays analysis was used to identify, describe and summarize evidence regarding the molecular basis of actions of ZOL and of their possible direct anti-tumour effects.We validated gene expression results of specific transcripts involved in major cellular process by Real Time and Western Blot analysis and we observed inhibition of proliferation and migration through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Matrigel assay.The up-regulation of these products may have an important role in inhibiting proliferation, invasion and angiogenesis mediated by ZOL.

View Article: PubMed Central - PubMed

Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.

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Effects of ZOL addition on MAPK and Akt-dependent pathways on MCF-7 cells. Cells were treated with 10 μM ZOL for 24, 48, 72 and 96 hrs. Thereafter, both the expression and activity of MAPK p44/42 and AKT were evaluated. Determination of the expression and phosphorylation of MAPK p44/42 and AKT evaluated as described in Materials and methods. Expression of the house-keeping protein GAPDH, used as loading control.
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fig02: Effects of ZOL addition on MAPK and Akt-dependent pathways on MCF-7 cells. Cells were treated with 10 μM ZOL for 24, 48, 72 and 96 hrs. Thereafter, both the expression and activity of MAPK p44/42 and AKT were evaluated. Determination of the expression and phosphorylation of MAPK p44/42 and AKT evaluated as described in Materials and methods. Expression of the house-keeping protein GAPDH, used as loading control.

Mentions: To elucidate the mechanisms by which cell proliferation is suppressed, we have analysed the effects of ZOL on specific proliferative pathways. Time-course experiments were performed using WB to determine phosphorylation and thus activation of MAPK and AKT pathways. We found that phosphorilation of MAPK and AKT was decreased significantly after both 24 hrs and 48 hrs exposure to 10 μM ZOL (Fig. 2).


Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.

Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini LR, Fanale D, Margarese N, Santini D, Bazan V, Russo A - J. Cell. Mol. Med. (2012)

Effects of ZOL addition on MAPK and Akt-dependent pathways on MCF-7 cells. Cells were treated with 10 μM ZOL for 24, 48, 72 and 96 hrs. Thereafter, both the expression and activity of MAPK p44/42 and AKT were evaluated. Determination of the expression and phosphorylation of MAPK p44/42 and AKT evaluated as described in Materials and methods. Expression of the house-keeping protein GAPDH, used as loading control.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822988&req=5

fig02: Effects of ZOL addition on MAPK and Akt-dependent pathways on MCF-7 cells. Cells were treated with 10 μM ZOL for 24, 48, 72 and 96 hrs. Thereafter, both the expression and activity of MAPK p44/42 and AKT were evaluated. Determination of the expression and phosphorylation of MAPK p44/42 and AKT evaluated as described in Materials and methods. Expression of the house-keeping protein GAPDH, used as loading control.
Mentions: To elucidate the mechanisms by which cell proliferation is suppressed, we have analysed the effects of ZOL on specific proliferative pathways. Time-course experiments were performed using WB to determine phosphorylation and thus activation of MAPK and AKT pathways. We found that phosphorilation of MAPK and AKT was decreased significantly after both 24 hrs and 48 hrs exposure to 10 μM ZOL (Fig. 2).

Bottom Line: Microarrays analysis was used to identify, describe and summarize evidence regarding the molecular basis of actions of ZOL and of their possible direct anti-tumour effects.We validated gene expression results of specific transcripts involved in major cellular process by Real Time and Western Blot analysis and we observed inhibition of proliferation and migration through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Matrigel assay.The up-regulation of these products may have an important role in inhibiting proliferation, invasion and angiogenesis mediated by ZOL.

View Article: PubMed Central - PubMed

Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.

Show MeSH
Related in: MedlinePlus