Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.
Bottom Line: We validated gene expression results of specific transcripts involved in major cellular process by Real Time and Western Blot analysis and we observed inhibition of proliferation and migration through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Matrigel assay.We then focused on changes in the cytoskeletal components as fibronectin 1 (FN1), actin, and anti angiogenic compounds as transforming growth factor-β1 (TGF-β1) and thrombospondin 1 (THBS1).The up-regulation of these products may have an important role in inhibiting proliferation, invasion and angiogenesis mediated by ZOL.
Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.Show MeSH
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Mentions: To elucidate the mechanisms by which cell proliferation is suppressed, we have analysed the effects of ZOL on specific proliferative pathways. Time-course experiments were performed using WB to determine phosphorylation and thus activation of MAPK and AKT pathways. We found that phosphorilation of MAPK and AKT was decreased significantly after both 24 hrs and 48 hrs exposure to 10 μM ZOL (Fig. 2).
Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.