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Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.

Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini LR, Fanale D, Margarese N, Santini D, Bazan V, Russo A - J. Cell. Mol. Med. (2012)

Bottom Line: Microarrays analysis was used to identify, describe and summarize evidence regarding the molecular basis of actions of ZOL and of their possible direct anti-tumour effects.We validated gene expression results of specific transcripts involved in major cellular process by Real Time and Western Blot analysis and we observed inhibition of proliferation and migration through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Matrigel assay.The up-regulation of these products may have an important role in inhibiting proliferation, invasion and angiogenesis mediated by ZOL.

View Article: PubMed Central - PubMed

Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.

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A, B ZOL inhibits cell growth in vitro. About 105 and 104 cells were cultured in 6-well and 96-well tissue culture plates and exposed to ZOL at a concentration ranging from 10 to 100 μM for different times. Cellular viability was analyzed by cellular count (A) and MTT assay (B).
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fig01: A, B ZOL inhibits cell growth in vitro. About 105 and 104 cells were cultured in 6-well and 96-well tissue culture plates and exposed to ZOL at a concentration ranging from 10 to 100 μM for different times. Cellular viability was analyzed by cellular count (A) and MTT assay (B).

Mentions: To identify the lower dose of ZOL sufficient to induce an anti-proliferative effect on MCF-7 cell proliferation, we tested different concentrations (10–100 μM) of ZOL for 24, 48 or 72 hrs.Cell count showed that cell growth was inhibited by ZOL versus control at all concentrations used (Fig. 1). In particular, tumour cell growth was reduced to about 40% at a ZOL concentration of 100 μM over a period of incubation of 24 hrs whereas the lower ZOL concentration (10 μM) was slightly less efficient (20%), but effective. Consider that inhibition rates of 10 and 100 μM of ZOL were not shown a significant difference, we can assert that the treatment at lower concentration for only 24 hrs is sufficient to induce an inhibition of proliferation, also confirmed by determination of number of metabolically active cells by MTT assay (Fig. 1A and B). On the basis of these data, we have selected this concentration of ZOL for all the subsequent experiments.


Analysis of molecular mechanisms and anti-tumoural effects of zoledronic acid in breast cancer cells.

Insalaco L, Di Gaudio F, Terrasi M, Amodeo V, Caruso S, Corsini LR, Fanale D, Margarese N, Santini D, Bazan V, Russo A - J. Cell. Mol. Med. (2012)

A, B ZOL inhibits cell growth in vitro. About 105 and 104 cells were cultured in 6-well and 96-well tissue culture plates and exposed to ZOL at a concentration ranging from 10 to 100 μM for different times. Cellular viability was analyzed by cellular count (A) and MTT assay (B).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822988&req=5

fig01: A, B ZOL inhibits cell growth in vitro. About 105 and 104 cells were cultured in 6-well and 96-well tissue culture plates and exposed to ZOL at a concentration ranging from 10 to 100 μM for different times. Cellular viability was analyzed by cellular count (A) and MTT assay (B).
Mentions: To identify the lower dose of ZOL sufficient to induce an anti-proliferative effect on MCF-7 cell proliferation, we tested different concentrations (10–100 μM) of ZOL for 24, 48 or 72 hrs.Cell count showed that cell growth was inhibited by ZOL versus control at all concentrations used (Fig. 1). In particular, tumour cell growth was reduced to about 40% at a ZOL concentration of 100 μM over a period of incubation of 24 hrs whereas the lower ZOL concentration (10 μM) was slightly less efficient (20%), but effective. Consider that inhibition rates of 10 and 100 μM of ZOL were not shown a significant difference, we can assert that the treatment at lower concentration for only 24 hrs is sufficient to induce an inhibition of proliferation, also confirmed by determination of number of metabolically active cells by MTT assay (Fig. 1A and B). On the basis of these data, we have selected this concentration of ZOL for all the subsequent experiments.

Bottom Line: Microarrays analysis was used to identify, describe and summarize evidence regarding the molecular basis of actions of ZOL and of their possible direct anti-tumour effects.We validated gene expression results of specific transcripts involved in major cellular process by Real Time and Western Blot analysis and we observed inhibition of proliferation and migration through 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and Matrigel assay.The up-regulation of these products may have an important role in inhibiting proliferation, invasion and angiogenesis mediated by ZOL.

View Article: PubMed Central - PubMed

Affiliation: Section of Medical Oncology, Department of Surgical and Oncology, University of Palermo, Palermo, Italy.

Show MeSH
Related in: MedlinePlus