Distinct overlapping sequences at the carboxy-terminus of merlin regulate its tumour suppressor and morphogenic activity.
Bottom Line: In addition to the two full-length merlin isoforms and truncating mutations found in patients, we focused on the evolutionally conserved C-terminal residues 545-547, also harbouring disease-causing mutations.The residues 538-568 were found particularly important for this morphogenic activity.The results further show that both merlin isoforms are able to equally inhibit proliferation, whereas C-terminal mutants affecting residues 545-547 are less effective in growth suppression.
Affiliation: Biomedicum Helsinki, Department of Pathology, University of Helsinki, Helsinki, Finland. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: To study the composition of the merlin-induced long protrusions, isoform 2 and 1-547 transfected Nf2−/− MEFs were stained for actin and tubulin. The merlin-induced long processes contained both actin and microtubuli (Fig. 3A), indicating that the extensions are not only actin-containing long filopodia, but represent other membrane structures.
Affiliation: Biomedicum Helsinki, Department of Pathology, University of Helsinki, Helsinki, Finland. email@example.com