Raf-1 levels determine the migration rate of primary endometrial stromal cells of patients with endometriosis.
Bottom Line: Raf-1 siRNA knockdown in Co- and Eu-hESC resulted in contraction and decreased migration versus siRNA controls.Lowest Raf-1 levels in Ec-hESC were associated with hyperactivated ROCKII and ezrin/radixin/moesin (E/R/M), impaired migration and a contracted phenotype similar to Raf-1 knockdown in Co- and Eu-hESC.In conclusion, our findings suggest that cellular levels of Raf-1 adjust the threshold of hESC migration in endometriosis.
Affiliation: Department of Obstetrics and Gynecology, Medical University of Vienna, Vienna, Austria.Show MeSH
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Mentions: To further analyse whether Raf-1 activity is required for MYPT1 inactivation, Eu-hESC were treated with Raf-1 kinase inhibitors ZM336372 and GW5074 (1 μM) for 24 hrs. The inhibition of Raf-1 reduced MYPT1 phosphorylation to approximately half (P < 0.05) of the levels of untreated cells (Fig. 5, upper panel), showing that Raf-1 kinase activity is required for the direct regulation of MYPT1 phosphorylation. Similar results were obtained for Co- and Ec-hESC under GW5074 administration (Fig. 5, lower panel).
Affiliation: Department of Obstetrics and Gynecology, Medical University of Vienna, Vienna, Austria.