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Identification of telocytes in the upper lamina propria of the human urinary tract.

Gevaert T, De Vos R, Van Der Aa F, Joniau S, van den Oord J, Roskams T, De Ridder D - J. Cell. Mol. Med. (2012)

Bottom Line: Tissue was obtained from normal areas in nephrectomy, cystectomy and prostatectomy specimens, and processed for morphology, immunohistochemistry and electron microscopy.The ULP IC in renal pelvis, ureter and urethra had a similar ultrastructural phenotype, which differed somehow from that of bladder IC, that is, thinner and longer cytoplasmic processes, no peripheral actin filaments and presence of dense core granules and microtubules.The functional relevance of ULP telocytes in the urinary tract remains to be elucidated, and ER and PR might therefore be promising pharmacological research targets.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, KU Leuven, University Hospitals Gasthuisberg, Leuven, Belgium. Thomas.Gevaert@med.kuleuven.be

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Electron micrograph of upper lamina propria (ULP) interstitial cells (IC) in renal pelvis, ureter, bladder and urethra. ULP IC in renal pelvis, ureter and urethra are mainly telocytes (arrows), characterized by long cytoplasmic prolongations, and called telopodes (red dashed lines). In bladder, ULP IC have a different phenotype, with shorter cytoplasmic prolongations, but are also identified as telocytes (arrows). In bladder, there are around 10–20 layers of ULP telocytes, while in the other urinary tract regions, ULP telocytes are only 2–3 rows thick. Scale bars = 7 μm. U: urothelial area.
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fig01: Electron micrograph of upper lamina propria (ULP) interstitial cells (IC) in renal pelvis, ureter, bladder and urethra. ULP IC in renal pelvis, ureter and urethra are mainly telocytes (arrows), characterized by long cytoplasmic prolongations, and called telopodes (red dashed lines). In bladder, ULP IC have a different phenotype, with shorter cytoplasmic prolongations, but are also identified as telocytes (arrows). In bladder, there are around 10–20 layers of ULP telocytes, while in the other urinary tract regions, ULP telocytes are only 2–3 rows thick. Scale bars = 7 μm. U: urothelial area.

Mentions: Ultrastructurally, ULP IC contained 2–3 layers of long cells with thin long cytoplasmic processes (at least 20–30 μm; Figs 1 and 2). These processes could occasionally present synapse-like thickenings. The cell body was rather small: the cytoplasm presented a moderate amount of organelles as rough endoplasmic reticulum cisternae and some mitochondria. Almost no cytoplasmic bundles of actin filaments with densifications were found. Some IC could contain microtubular structures and dense core vesicles. At the peripheral cell membrane, caveolae were seen; plasmalemmal thickenings and fibronexus were not obvious (Fig. 3). Intercellular connections consisted mostly of intermediate and gap-like junctions. Only occasional interlaying axonal nerve endings were observed. Close relationship with superficial capillaries was observed (Figs 2). The ULP IC population was intermingled with sparse fibroblasts (see Table 2 for an overview).


Identification of telocytes in the upper lamina propria of the human urinary tract.

Gevaert T, De Vos R, Van Der Aa F, Joniau S, van den Oord J, Roskams T, De Ridder D - J. Cell. Mol. Med. (2012)

Electron micrograph of upper lamina propria (ULP) interstitial cells (IC) in renal pelvis, ureter, bladder and urethra. ULP IC in renal pelvis, ureter and urethra are mainly telocytes (arrows), characterized by long cytoplasmic prolongations, and called telopodes (red dashed lines). In bladder, ULP IC have a different phenotype, with shorter cytoplasmic prolongations, but are also identified as telocytes (arrows). In bladder, there are around 10–20 layers of ULP telocytes, while in the other urinary tract regions, ULP telocytes are only 2–3 rows thick. Scale bars = 7 μm. U: urothelial area.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822978&req=5

fig01: Electron micrograph of upper lamina propria (ULP) interstitial cells (IC) in renal pelvis, ureter, bladder and urethra. ULP IC in renal pelvis, ureter and urethra are mainly telocytes (arrows), characterized by long cytoplasmic prolongations, and called telopodes (red dashed lines). In bladder, ULP IC have a different phenotype, with shorter cytoplasmic prolongations, but are also identified as telocytes (arrows). In bladder, there are around 10–20 layers of ULP telocytes, while in the other urinary tract regions, ULP telocytes are only 2–3 rows thick. Scale bars = 7 μm. U: urothelial area.
Mentions: Ultrastructurally, ULP IC contained 2–3 layers of long cells with thin long cytoplasmic processes (at least 20–30 μm; Figs 1 and 2). These processes could occasionally present synapse-like thickenings. The cell body was rather small: the cytoplasm presented a moderate amount of organelles as rough endoplasmic reticulum cisternae and some mitochondria. Almost no cytoplasmic bundles of actin filaments with densifications were found. Some IC could contain microtubular structures and dense core vesicles. At the peripheral cell membrane, caveolae were seen; plasmalemmal thickenings and fibronexus were not obvious (Fig. 3). Intercellular connections consisted mostly of intermediate and gap-like junctions. Only occasional interlaying axonal nerve endings were observed. Close relationship with superficial capillaries was observed (Figs 2). The ULP IC population was intermingled with sparse fibroblasts (see Table 2 for an overview).

Bottom Line: Tissue was obtained from normal areas in nephrectomy, cystectomy and prostatectomy specimens, and processed for morphology, immunohistochemistry and electron microscopy.The ULP IC in renal pelvis, ureter and urethra had a similar ultrastructural phenotype, which differed somehow from that of bladder IC, that is, thinner and longer cytoplasmic processes, no peripheral actin filaments and presence of dense core granules and microtubules.The functional relevance of ULP telocytes in the urinary tract remains to be elucidated, and ER and PR might therefore be promising pharmacological research targets.

View Article: PubMed Central - PubMed

Affiliation: Department of Urology, KU Leuven, University Hospitals Gasthuisberg, Leuven, Belgium. Thomas.Gevaert@med.kuleuven.be

Show MeSH
Related in: MedlinePlus