Cardiomyocytes derived from human embryonic and induced pluripotent stem cells: comparative ultrastructure.
Bottom Line: Noteworthy, the SR is less developed in HFKT-iPSC-CM.We also found in both cell types: (1) 'Ca(2+)-release units', which connect the peripheral sarcoplasmic reticulum with plasmalemma; and (2) intercellular junctions, which mimic intercalated disks (desmosomes and fascia adherens).In conclusion, iPSC and hESC differentiate into cardiomyocytes of comparable ultrastructure, thus supporting the notion that iPSC offer a viable option for an autologous cell source for cardiac regenerative therapy.
Affiliation: Victor Babeş National Institute of Pathology, Bucharest, Romania.Show MeSH
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Mentions: Both HFKT-iPSC-CM (Figs 3A and 4A) and hESC-CM (Figs 3B and 4B) showed ultrastructural features of early (centre of EB; Fig. 4) and immature cardiac phenotype (periphery of EB; Fig. 3) containing myofibrils (Figs 3, 4 and 6–14), large glycogen deposits (Figs 4 and 9), lipid droplets (Figs 3 and 4), mitochondria (Figs 3, 4 and 8), rough endoplasmic reticulum (Fig. 10), sarcoplasmic reticulum (Figs 7 and 11–13) and caveolae (Fig. 13). Noteworthy, intercellular junctions (Figs 6 and 14) were formed by desmosomes and fascia adherens.
Affiliation: Victor Babeş National Institute of Pathology, Bucharest, Romania.