Cardiomyocytes derived from human embryonic and induced pluripotent stem cells: comparative ultrastructure.
Bottom Line: Noteworthy, the SR is less developed in HFKT-iPSC-CM.We also found in both cell types: (1) 'Ca(2+)-release units', which connect the peripheral sarcoplasmic reticulum with plasmalemma; and (2) intercellular junctions, which mimic intercalated disks (desmosomes and fascia adherens).In conclusion, iPSC and hESC differentiate into cardiomyocytes of comparable ultrastructure, thus supporting the notion that iPSC offer a viable option for an autologous cell source for cardiac regenerative therapy.
Affiliation: Victor Babeş National Institute of Pathology, Bucharest, Romania.Show MeSH
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Mentions: The functionality of the iPSC-CM is illustrated by robust extracellular electrograms recorded by the MEA data acquisition system (Fig. 1A). In addition, the functionality of these cells is also demonstrated by representative recordings of spontaneous generated action potentials with prominent pacemaker potential (Fig. 1B). Similar results were obtained in eight preparations and were previously described .
Affiliation: Victor Babeş National Institute of Pathology, Bucharest, Romania.