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Cardiomyocytes derived from human embryonic and induced pluripotent stem cells: comparative ultrastructure.

Gherghiceanu M, Barad L, Novak A, Reiter I, Itskovitz-Eldor J, Binah O, Popescu LM - J. Cell. Mol. Med. (2011)

Bottom Line: Noteworthy, the SR is less developed in HFKT-iPSC-CM.We also found in both cell types: (1) 'Ca(2+)-release units', which connect the peripheral sarcoplasmic reticulum with plasmalemma; and (2) intercellular junctions, which mimic intercalated disks (desmosomes and fascia adherens).In conclusion, iPSC and hESC differentiate into cardiomyocytes of comparable ultrastructure, thus supporting the notion that iPSC offer a viable option for an autologous cell source for cardiac regenerative therapy.

View Article: PubMed Central - PubMed

Affiliation: Victor Babeş National Institute of Pathology, Bucharest, Romania.

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Electrophysiological features of cardiomyocytes derived from iPSC generated from human plucked hair follicle keratinocytes (HFKT-iPSC-CM; clone KTN3). (A) Extracellular electrograms recorded by means of MEA data acquisition system from a spontaneously contracting embryoid body (EB). (B) Representative action potential recordings from a spontaneously contracting EB (clone KTN3), demonstrating the pacemaker activity of the HFKT-iPSC-CM.
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fig01: Electrophysiological features of cardiomyocytes derived from iPSC generated from human plucked hair follicle keratinocytes (HFKT-iPSC-CM; clone KTN3). (A) Extracellular electrograms recorded by means of MEA data acquisition system from a spontaneously contracting embryoid body (EB). (B) Representative action potential recordings from a spontaneously contracting EB (clone KTN3), demonstrating the pacemaker activity of the HFKT-iPSC-CM.

Mentions: The functionality of the iPSC-CM is illustrated by robust extracellular electrograms recorded by the MEA data acquisition system (Fig. 1A). In addition, the functionality of these cells is also demonstrated by representative recordings of spontaneous generated action potentials with prominent pacemaker potential (Fig. 1B). Similar results were obtained in eight preparations and were previously described [4].


Cardiomyocytes derived from human embryonic and induced pluripotent stem cells: comparative ultrastructure.

Gherghiceanu M, Barad L, Novak A, Reiter I, Itskovitz-Eldor J, Binah O, Popescu LM - J. Cell. Mol. Med. (2011)

Electrophysiological features of cardiomyocytes derived from iPSC generated from human plucked hair follicle keratinocytes (HFKT-iPSC-CM; clone KTN3). (A) Extracellular electrograms recorded by means of MEA data acquisition system from a spontaneously contracting embryoid body (EB). (B) Representative action potential recordings from a spontaneously contracting EB (clone KTN3), demonstrating the pacemaker activity of the HFKT-iPSC-CM.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822963&req=5

fig01: Electrophysiological features of cardiomyocytes derived from iPSC generated from human plucked hair follicle keratinocytes (HFKT-iPSC-CM; clone KTN3). (A) Extracellular electrograms recorded by means of MEA data acquisition system from a spontaneously contracting embryoid body (EB). (B) Representative action potential recordings from a spontaneously contracting EB (clone KTN3), demonstrating the pacemaker activity of the HFKT-iPSC-CM.
Mentions: The functionality of the iPSC-CM is illustrated by robust extracellular electrograms recorded by the MEA data acquisition system (Fig. 1A). In addition, the functionality of these cells is also demonstrated by representative recordings of spontaneous generated action potentials with prominent pacemaker potential (Fig. 1B). Similar results were obtained in eight preparations and were previously described [4].

Bottom Line: Noteworthy, the SR is less developed in HFKT-iPSC-CM.We also found in both cell types: (1) 'Ca(2+)-release units', which connect the peripheral sarcoplasmic reticulum with plasmalemma; and (2) intercellular junctions, which mimic intercalated disks (desmosomes and fascia adherens).In conclusion, iPSC and hESC differentiate into cardiomyocytes of comparable ultrastructure, thus supporting the notion that iPSC offer a viable option for an autologous cell source for cardiac regenerative therapy.

View Article: PubMed Central - PubMed

Affiliation: Victor Babeş National Institute of Pathology, Bucharest, Romania.

Show MeSH
Related in: MedlinePlus