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NK receptors, Substance P, Ano1 expression and ultrastructural features of the muscle coat in Cav-1(-/-) mouse ileum.

Cipriani G, Serboiu CS, Gherghiceanu M, Faussone-Pellegrini MS, Vannucchi MG - J. Cell. Mol. Med. (2011)

Bottom Line: ICC were also labelled with c-Kit and tachykinergic neurons with Substance P (SP).Under TEM: (i) ICC, SMC and telocytes lacked typical caveolae but had few and large flask-shaped vesicles we called large-sized caveolae; (ii) SMC and ICC contained an extraordinary high number of mitochondria, (iii) neurons were unchanged.Loss of Ano-1 expression, decrease of SP content and consequently overexpression of NK receptors suggest that all these molecules are Cav-1-associated proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Histology and Forensic Medicine, Section of Histology, University of Florence, Florence, Italy.

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(A, B) Cav-1-IR. In the control (A), Cav-1-IR appears as small bars distributed along the plasma membrane on the entire cell contour. In the Cav-1−/− mice (B), Cav-1-IR can be detected, although having a very low intensity. (C, D) c-kit-IR. In the control (C), both the ICC at the myenteric plexus (MP) and the deep muscular plexus (DMP) are c-kit-IR; in the Cav-1−/− mice (D), the c-kit-IR is perfectly maintained. (E, F) Ano1-IR. In the control (E), all ICC show the Ano1-IR whereas in the Cav-1−/− mice (F) these cells are Ano1-negative. Bar = 40 μm.
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fig01: (A, B) Cav-1-IR. In the control (A), Cav-1-IR appears as small bars distributed along the plasma membrane on the entire cell contour. In the Cav-1−/− mice (B), Cav-1-IR can be detected, although having a very low intensity. (C, D) c-kit-IR. In the control (C), both the ICC at the myenteric plexus (MP) and the deep muscular plexus (DMP) are c-kit-IR; in the Cav-1−/− mice (D), the c-kit-IR is perfectly maintained. (E, F) Ano1-IR. In the control (E), all ICC show the Ano1-IR whereas in the Cav-1−/− mice (F) these cells are Ano1-negative. Bar = 40 μm.

Mentions: Cav-1 immunoreactivity (IR) in controls appeared as small bars distributed along the plasma membrane on the entire cell contour (Fig. 1A). In the sections from Cav-1−/− mice labelled with fluorescent antibodies, this positivity was not appreciable, whereas in those labelled for bright field microscopy, Cav-1-positivity could still be detected although at a very low intensity (Fig. 1B).


NK receptors, Substance P, Ano1 expression and ultrastructural features of the muscle coat in Cav-1(-/-) mouse ileum.

Cipriani G, Serboiu CS, Gherghiceanu M, Faussone-Pellegrini MS, Vannucchi MG - J. Cell. Mol. Med. (2011)

(A, B) Cav-1-IR. In the control (A), Cav-1-IR appears as small bars distributed along the plasma membrane on the entire cell contour. In the Cav-1−/− mice (B), Cav-1-IR can be detected, although having a very low intensity. (C, D) c-kit-IR. In the control (C), both the ICC at the myenteric plexus (MP) and the deep muscular plexus (DMP) are c-kit-IR; in the Cav-1−/− mice (D), the c-kit-IR is perfectly maintained. (E, F) Ano1-IR. In the control (E), all ICC show the Ano1-IR whereas in the Cav-1−/− mice (F) these cells are Ano1-negative. Bar = 40 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822952&req=5

fig01: (A, B) Cav-1-IR. In the control (A), Cav-1-IR appears as small bars distributed along the plasma membrane on the entire cell contour. In the Cav-1−/− mice (B), Cav-1-IR can be detected, although having a very low intensity. (C, D) c-kit-IR. In the control (C), both the ICC at the myenteric plexus (MP) and the deep muscular plexus (DMP) are c-kit-IR; in the Cav-1−/− mice (D), the c-kit-IR is perfectly maintained. (E, F) Ano1-IR. In the control (E), all ICC show the Ano1-IR whereas in the Cav-1−/− mice (F) these cells are Ano1-negative. Bar = 40 μm.
Mentions: Cav-1 immunoreactivity (IR) in controls appeared as small bars distributed along the plasma membrane on the entire cell contour (Fig. 1A). In the sections from Cav-1−/− mice labelled with fluorescent antibodies, this positivity was not appreciable, whereas in those labelled for bright field microscopy, Cav-1-positivity could still be detected although at a very low intensity (Fig. 1B).

Bottom Line: ICC were also labelled with c-Kit and tachykinergic neurons with Substance P (SP).Under TEM: (i) ICC, SMC and telocytes lacked typical caveolae but had few and large flask-shaped vesicles we called large-sized caveolae; (ii) SMC and ICC contained an extraordinary high number of mitochondria, (iii) neurons were unchanged.Loss of Ano-1 expression, decrease of SP content and consequently overexpression of NK receptors suggest that all these molecules are Cav-1-associated proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Histology and Forensic Medicine, Section of Histology, University of Florence, Florence, Italy.

Show MeSH
Related in: MedlinePlus