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Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function.

Greulich S, de Wiza DH, Preilowski S, Ding Z, Mueller H, Langin D, Jaquet K, Ouwens DM, Eckel J - J. Cell. Mol. Med. (2011)

Bottom Line: Eleven factors were differentially secreted by EAT when compared to SAT.In cardiomyocytes, CM from EAT of HFD-fed animals increased SMAD2-phosphorylation, a marker for activin A-signalling, decreased sarcoplasmic-endoplasmic reticulum calcium ATPase 2a expression, and reduced insulin-mediated phosphorylation of Akt-Ser473 versus CM from SAT and standard diet-fed animals.Finally, CM from EAT of HFD-fed animals as compared to CM from the other groups markedly reduced sarcomere shortening and cytosolic Ca(2+) fluxes in cardiomyocytes.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Centre, Düsseldorf, Germany.

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Effect of CM from EAT and SAT on SERCA2a expression. Lysates prepared from rat cardiomyocytes incubated with control AM or CM (diluted 1:4) from EAT and SAT from SD- and HFD-fed guinea pigs for 24 hrs were analysed by Western blotting for SERCA2a- and α-tubulin expression. Representative Western blots (A) and quantitative analysis (B) are also shown. Open bars: AM; grey bars: CM from SD-fed animals; black bars: CM from HFD-fed animals. Data are expressed as mean ± S.E.M. (n= 3 per group). Differences between the experimental groups were calculated by one-way anova and unpaired Student’s t-tests. #P < 0.05 versus AM; *P < 0.05 HFD versus SD.
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fig07: Effect of CM from EAT and SAT on SERCA2a expression. Lysates prepared from rat cardiomyocytes incubated with control AM or CM (diluted 1:4) from EAT and SAT from SD- and HFD-fed guinea pigs for 24 hrs were analysed by Western blotting for SERCA2a- and α-tubulin expression. Representative Western blots (A) and quantitative analysis (B) are also shown. Open bars: AM; grey bars: CM from SD-fed animals; black bars: CM from HFD-fed animals. Data are expressed as mean ± S.E.M. (n= 3 per group). Differences between the experimental groups were calculated by one-way anova and unpaired Student’s t-tests. #P < 0.05 versus AM; *P < 0.05 HFD versus SD.

Mentions: Expression of SERCA2a, a key regulator of cardiac Ca2+ metabolism, was reduced in cardiomyocytes incubated with CM-EAT from HFD-fed animals when compared to AM and CM-EAT from SD-fed animals. CM-SAT from HFD-fed animals also slightly lowered SERCA2a expression whereas CM from the other groups had no effect (Fig. 7). Thus, the detrimental effects of CM-EAT from HFD-fed animals on contractile function are paralleled by a reduction in SERCA2a expression.


Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function.

Greulich S, de Wiza DH, Preilowski S, Ding Z, Mueller H, Langin D, Jaquet K, Ouwens DM, Eckel J - J. Cell. Mol. Med. (2011)

Effect of CM from EAT and SAT on SERCA2a expression. Lysates prepared from rat cardiomyocytes incubated with control AM or CM (diluted 1:4) from EAT and SAT from SD- and HFD-fed guinea pigs for 24 hrs were analysed by Western blotting for SERCA2a- and α-tubulin expression. Representative Western blots (A) and quantitative analysis (B) are also shown. Open bars: AM; grey bars: CM from SD-fed animals; black bars: CM from HFD-fed animals. Data are expressed as mean ± S.E.M. (n= 3 per group). Differences between the experimental groups were calculated by one-way anova and unpaired Student’s t-tests. #P < 0.05 versus AM; *P < 0.05 HFD versus SD.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822951&req=5

fig07: Effect of CM from EAT and SAT on SERCA2a expression. Lysates prepared from rat cardiomyocytes incubated with control AM or CM (diluted 1:4) from EAT and SAT from SD- and HFD-fed guinea pigs for 24 hrs were analysed by Western blotting for SERCA2a- and α-tubulin expression. Representative Western blots (A) and quantitative analysis (B) are also shown. Open bars: AM; grey bars: CM from SD-fed animals; black bars: CM from HFD-fed animals. Data are expressed as mean ± S.E.M. (n= 3 per group). Differences between the experimental groups were calculated by one-way anova and unpaired Student’s t-tests. #P < 0.05 versus AM; *P < 0.05 HFD versus SD.
Mentions: Expression of SERCA2a, a key regulator of cardiac Ca2+ metabolism, was reduced in cardiomyocytes incubated with CM-EAT from HFD-fed animals when compared to AM and CM-EAT from SD-fed animals. CM-SAT from HFD-fed animals also slightly lowered SERCA2a expression whereas CM from the other groups had no effect (Fig. 7). Thus, the detrimental effects of CM-EAT from HFD-fed animals on contractile function are paralleled by a reduction in SERCA2a expression.

Bottom Line: Eleven factors were differentially secreted by EAT when compared to SAT.In cardiomyocytes, CM from EAT of HFD-fed animals increased SMAD2-phosphorylation, a marker for activin A-signalling, decreased sarcoplasmic-endoplasmic reticulum calcium ATPase 2a expression, and reduced insulin-mediated phosphorylation of Akt-Ser473 versus CM from SAT and standard diet-fed animals.Finally, CM from EAT of HFD-fed animals as compared to CM from the other groups markedly reduced sarcomere shortening and cytosolic Ca(2+) fluxes in cardiomyocytes.

View Article: PubMed Central - PubMed

Affiliation: Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Centre, Düsseldorf, Germany.

Show MeSH
Related in: MedlinePlus