Secretory products of guinea pig epicardial fat induce insulin resistance and impair primary adult rat cardiomyocyte function.
Bottom Line: Eleven factors were differentially secreted by EAT when compared to SAT.In cardiomyocytes, CM from EAT of HFD-fed animals increased SMAD2-phosphorylation, a marker for activin A-signalling, decreased sarcoplasmic-endoplasmic reticulum calcium ATPase 2a expression, and reduced insulin-mediated phosphorylation of Akt-Ser473 versus CM from SAT and standard diet-fed animals.Finally, CM from EAT of HFD-fed animals as compared to CM from the other groups markedly reduced sarcomere shortening and cytosolic Ca(2+) fluxes in cardiomyocytes.
Affiliation: Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Centre, Düsseldorf, Germany.Show MeSH
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Mentions: Expression of SERCA2a, a key regulator of cardiac Ca2+ metabolism, was reduced in cardiomyocytes incubated with CM-EAT from HFD-fed animals when compared to AM and CM-EAT from SD-fed animals. CM-SAT from HFD-fed animals also slightly lowered SERCA2a expression whereas CM from the other groups had no effect (Fig. 7). Thus, the detrimental effects of CM-EAT from HFD-fed animals on contractile function are paralleled by a reduction in SERCA2a expression.
Affiliation: Institute of Clinical Biochemistry and Pathobiochemistry, German Diabetes Centre, Düsseldorf, Germany.