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The anticancer flavonoid chrysin induces the unfolded protein response in hepatoma cells.

Sun X, Huo X, Luo T, Li M, Yin Y, Jiang Y - J. Cell. Mol. Med. (2011)

Bottom Line: Chrysin is a natural and biologically active flavonoid with anticancer effects.Abrogation of GRP78 induction may improve the anticancer effects of chrysin.Combination of EGCG and chrysin represents a new regimen for cancer chemoprevention and therapeutics.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biotherapy, Section of Signal Transduction and Molecular Targeted Therapy, West China Hospital, Sichuan University, Chengdu, China.

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EGCG potentiates chrysin-induced apoptosis. (A) HepG2 cells were treated with or without 20 μM EGCG, 10 μM chrysin and 20 μM z-VAD-fmk for 48 hrs. Apoptosis was assessed by Hoechst 33342 staining. Red arrow: representatives of apoptotic cells. Quantification of apoptotic cells was performed by taking the images in random fields and counting cells with strong fluorescence, condensed or fragmented nuclei. The apoptosis rate was plotted. Columns: mean percentage of apoptotic cells; bars: S.E. (B) SMMC-7721 cells were treated with or without 20 μM EGCG, 20 μM chrysin and 20 μM z-VAD-fmk for 48 hrs. Apoptosis was assessed by Hoechst 33342 staining. Red arrow: representatives of apoptotic cells. The apoptosis rate was plotted. Columns: mean percentage of apoptotic cells; bars: S.E. A representative of three experiments was shown.
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fig08: EGCG potentiates chrysin-induced apoptosis. (A) HepG2 cells were treated with or without 20 μM EGCG, 10 μM chrysin and 20 μM z-VAD-fmk for 48 hrs. Apoptosis was assessed by Hoechst 33342 staining. Red arrow: representatives of apoptotic cells. Quantification of apoptotic cells was performed by taking the images in random fields and counting cells with strong fluorescence, condensed or fragmented nuclei. The apoptosis rate was plotted. Columns: mean percentage of apoptotic cells; bars: S.E. (B) SMMC-7721 cells were treated with or without 20 μM EGCG, 20 μM chrysin and 20 μM z-VAD-fmk for 48 hrs. Apoptosis was assessed by Hoechst 33342 staining. Red arrow: representatives of apoptotic cells. The apoptosis rate was plotted. Columns: mean percentage of apoptotic cells; bars: S.E. A representative of three experiments was shown.

Mentions: To determine whether EGCG potentiated chrysin-induced apoptosis, HepG2 cells were treated with EGCG, chrysin or both for 48 hrs, followed by Hoechst 33342 staining. Whereas EGCG alone had little effects on apoptosis, combination of EGCG and chrysin resulted in significantly higher apoptotic rate than chrysin alone. The stimulatory effects of EGCG on chrysin-induced apoptosis could be abrogated by caspase inhibitor (Fig. 8A). EGCG also potentiated chrysin-induced apoptosis in SMMC-7721 cells (Fig. 8B). These data indicated that EGCG could potentiate chrysin-induced apoptosis through caspase activation.


The anticancer flavonoid chrysin induces the unfolded protein response in hepatoma cells.

Sun X, Huo X, Luo T, Li M, Yin Y, Jiang Y - J. Cell. Mol. Med. (2011)

EGCG potentiates chrysin-induced apoptosis. (A) HepG2 cells were treated with or without 20 μM EGCG, 10 μM chrysin and 20 μM z-VAD-fmk for 48 hrs. Apoptosis was assessed by Hoechst 33342 staining. Red arrow: representatives of apoptotic cells. Quantification of apoptotic cells was performed by taking the images in random fields and counting cells with strong fluorescence, condensed or fragmented nuclei. The apoptosis rate was plotted. Columns: mean percentage of apoptotic cells; bars: S.E. (B) SMMC-7721 cells were treated with or without 20 μM EGCG, 20 μM chrysin and 20 μM z-VAD-fmk for 48 hrs. Apoptosis was assessed by Hoechst 33342 staining. Red arrow: representatives of apoptotic cells. The apoptosis rate was plotted. Columns: mean percentage of apoptotic cells; bars: S.E. A representative of three experiments was shown.
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Related In: Results  -  Collection

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fig08: EGCG potentiates chrysin-induced apoptosis. (A) HepG2 cells were treated with or without 20 μM EGCG, 10 μM chrysin and 20 μM z-VAD-fmk for 48 hrs. Apoptosis was assessed by Hoechst 33342 staining. Red arrow: representatives of apoptotic cells. Quantification of apoptotic cells was performed by taking the images in random fields and counting cells with strong fluorescence, condensed or fragmented nuclei. The apoptosis rate was plotted. Columns: mean percentage of apoptotic cells; bars: S.E. (B) SMMC-7721 cells were treated with or without 20 μM EGCG, 20 μM chrysin and 20 μM z-VAD-fmk for 48 hrs. Apoptosis was assessed by Hoechst 33342 staining. Red arrow: representatives of apoptotic cells. The apoptosis rate was plotted. Columns: mean percentage of apoptotic cells; bars: S.E. A representative of three experiments was shown.
Mentions: To determine whether EGCG potentiated chrysin-induced apoptosis, HepG2 cells were treated with EGCG, chrysin or both for 48 hrs, followed by Hoechst 33342 staining. Whereas EGCG alone had little effects on apoptosis, combination of EGCG and chrysin resulted in significantly higher apoptotic rate than chrysin alone. The stimulatory effects of EGCG on chrysin-induced apoptosis could be abrogated by caspase inhibitor (Fig. 8A). EGCG also potentiated chrysin-induced apoptosis in SMMC-7721 cells (Fig. 8B). These data indicated that EGCG could potentiate chrysin-induced apoptosis through caspase activation.

Bottom Line: Chrysin is a natural and biologically active flavonoid with anticancer effects.Abrogation of GRP78 induction may improve the anticancer effects of chrysin.Combination of EGCG and chrysin represents a new regimen for cancer chemoprevention and therapeutics.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Biotherapy, Section of Signal Transduction and Molecular Targeted Therapy, West China Hospital, Sichuan University, Chengdu, China.

Show MeSH
Related in: MedlinePlus