The anticancer flavonoid chrysin induces the unfolded protein response in hepatoma cells.
Bottom Line: Chrysin is a natural and biologically active flavonoid with anticancer effects.Abrogation of GRP78 induction may improve the anticancer effects of chrysin.Combination of EGCG and chrysin represents a new regimen for cancer chemoprevention and therapeutics.
Affiliation: State Key Laboratory of Biotherapy, Section of Signal Transduction and Molecular Targeted Therapy, West China Hospital, Sichuan University, Chengdu, China.Show MeSH
Related in: MedlinePlus
Mentions: To determine whether EGCG potentiated chrysin-induced apoptosis, HepG2 cells were treated with EGCG, chrysin or both for 48 hrs, followed by Hoechst 33342 staining. Whereas EGCG alone had little effects on apoptosis, combination of EGCG and chrysin resulted in significantly higher apoptotic rate than chrysin alone. The stimulatory effects of EGCG on chrysin-induced apoptosis could be abrogated by caspase inhibitor (Fig. 8A). EGCG also potentiated chrysin-induced apoptosis in SMMC-7721 cells (Fig. 8B). These data indicated that EGCG could potentiate chrysin-induced apoptosis through caspase activation.
Affiliation: State Key Laboratory of Biotherapy, Section of Signal Transduction and Molecular Targeted Therapy, West China Hospital, Sichuan University, Chengdu, China.