Immunosuppressive effect of bone marrow-derived mesenchymal stem cells in inflammatory microenvironment favours the growth of B16 melanoma cells.
Bottom Line: We first compared the promotive capacity of bone marrow-derived MSCs on B16 melanoma cells growth in vivo, pre-incubated or not with the inflammatory cytokines interferon (IFN)-γ and tumour necrosis factor (TNF)-α.These cytokine combinations provoke the expression of inducible nitric oxide synthase (iNOS) by MSCs.The impulsive effect of MSCs treated with inflammatory cytokines on B16 melanoma cells in vivo can be reversed by inhibitor or short interfering RNA of iNOS.
Affiliation: Tumor Immunology and Gene Therapy Center, Eastern Hepatobiliary Surgery Hospital, The Second Military Medicial University, Shanghai, China.Show MeSH
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Mentions: MSCs adhered to the plastic surface, presenting a small population of single cells which were spindle-shaped and contained one nucleus after 72 hrs of the primary culture. During 7 to 10 days after the initial plating, the cells looked like long spindle-shaped fibroblastic cells and began to form colonies. MSCs could differentiate into adipocytes and osteoblast-like cells. The surface antigen profile of mouse MSCs was detected by flow cytometry was positive for CD90, CD105 and CD29 and negative for CD34 and CD45 (Fig. 1A, B)
Affiliation: Tumor Immunology and Gene Therapy Center, Eastern Hepatobiliary Surgery Hospital, The Second Military Medicial University, Shanghai, China.