Catalase activity prevents exercise-induced up-regulation of vasoprotective proteins in venous tissue.
Bottom Line: Physical activity induces favourable changes of arterial gene expression and protein activity, although little is known about its effect in venous tissue.Both training protocols similarly increased relative heart weight and resulted in up-regulation of aortic and myocardial eNOS.Furthermore, therapeutic inhibition of vascular catalase might improve pulmonary rehabilitation.
Affiliation: Institute for Pharmacology and Clinical Pharmacology, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany.Show MeSH
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Mentions: A total of 60 male C57BL/6 mice, 2–3 months of age were used. All mice were bred at the university’s animal facilities in a specified pathogen-free area and were singularized for 8 weeks prior exercise training. Sedentary mice and forced exercise mice lived individually in small cages (0.036 m2) for the whole experimental period of 12 weeks, voluntary running mice were transferred after 8 weeks of singularization in 0.036 m2 cages to larger cages and remained there for 4 weeks. These cages offered a floor space of 0.051 m2 and were supplied with running wheels as described below (Fig. 1A). Groups of sedentary mice, forced exercise mice and voluntary running mice (n= 8 each) were used for the initial investigation. Additional 20 male C57BL/6 mice underwent voluntary running to obtain further data in vena cava and 16 male C57BL/6 mice were treated with the catalase inhibitor aminotriazole (Sigma, Munich, Germany) for 6 weeks and 12 underwent voluntary running during the last 4 weeks. All mice were killed and the tissues were snap-frozen in liquid nitrogen.
Affiliation: Institute for Pharmacology and Clinical Pharmacology, University Hospital, Heinrich-Heine-University, Duesseldorf, Germany.