Limits...
Experimental acute myocardial infarction: telocytes involvement in neo-angiogenesis.

Manole CG, Cismaşiu V, Gherghiceanu M, Popescu LM - J. Cell. Mol. Med. (2011)

Bottom Line: Higher resolution images provided the following information: (a) the intercellular space between the abluminal face of endothelium and its surrounding TCs is frequently less than 50 nm; (b) TCs establish multiple direct nanocontacts with endothelial cells, where the extracellular space seems obliterated; such nanocontacts have a length of 0.4-1.5 μm; (c) the absence of basal membrane on the abluminal face of endothelial cell.Besides the physical contacts (either nanoscopic or microscopic) TCs presumably contribute to neo-angiognesis via paracrine secretion (as shown by immunocytochemistry for VEGF or NOS2).Last but not least, TCs contain measurable quantities of angiogenic microRNAs (e.g. let-7e, 10a, 21, 27b, 100, 126-3p, 130a, 143, 155, 503).

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.

Show MeSH

Related in: MedlinePlus

Rat experimental myocardial infarction. Border zone: 30-day-old. Transmission electron microscopy. This low magnification view shows four cardiomyocytes (CM), two blood capillaries (1 and 2) and numerous telocytes (TC) with long and slender telopodes (Tp). Note the close spatial relationship between TC/Tp and capillary-1 wall (endothelium). Capillary-1 is presumably a neo-capillary created in the interstitial space. Capillary-2, between three cardiomyocytes (CMs), has a TC and Tps in the vicinity, but the distance between abluminal membrane of endothelium and TC/Tp plasma membrane is wider. Thus, capillary-2 is probably an ‘old’ capillary.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3822940&req=5

fig04: Rat experimental myocardial infarction. Border zone: 30-day-old. Transmission electron microscopy. This low magnification view shows four cardiomyocytes (CM), two blood capillaries (1 and 2) and numerous telocytes (TC) with long and slender telopodes (Tp). Note the close spatial relationship between TC/Tp and capillary-1 wall (endothelium). Capillary-1 is presumably a neo-capillary created in the interstitial space. Capillary-2, between three cardiomyocytes (CMs), has a TC and Tps in the vicinity, but the distance between abluminal membrane of endothelium and TC/Tp plasma membrane is wider. Thus, capillary-2 is probably an ‘old’ capillary.

Mentions: Thirty days after the LADC ligation, a process of recovering characterizes the border zone of the myocardial infarction (Fig. 4). CMs begin to have normal ultrastructural appearance. In the interstitium, the number of TCs is clearly increased in comparison with normal myocardium. TCs/Tps have close spatial relationships with blood capillaries. However, the distances separating the Tps and abluminal front of endothelial cells and Tps are different for capillary of neo-formation (e.g. capillary-1 in Fig. 4) versus preexisting capillaries (e.g. capillary-2 in Fig. 4). Digitally measurements of the nanoscopic and microscopic distances resulted in the following results:


Experimental acute myocardial infarction: telocytes involvement in neo-angiogenesis.

Manole CG, Cismaşiu V, Gherghiceanu M, Popescu LM - J. Cell. Mol. Med. (2011)

Rat experimental myocardial infarction. Border zone: 30-day-old. Transmission electron microscopy. This low magnification view shows four cardiomyocytes (CM), two blood capillaries (1 and 2) and numerous telocytes (TC) with long and slender telopodes (Tp). Note the close spatial relationship between TC/Tp and capillary-1 wall (endothelium). Capillary-1 is presumably a neo-capillary created in the interstitial space. Capillary-2, between three cardiomyocytes (CMs), has a TC and Tps in the vicinity, but the distance between abluminal membrane of endothelium and TC/Tp plasma membrane is wider. Thus, capillary-2 is probably an ‘old’ capillary.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822940&req=5

fig04: Rat experimental myocardial infarction. Border zone: 30-day-old. Transmission electron microscopy. This low magnification view shows four cardiomyocytes (CM), two blood capillaries (1 and 2) and numerous telocytes (TC) with long and slender telopodes (Tp). Note the close spatial relationship between TC/Tp and capillary-1 wall (endothelium). Capillary-1 is presumably a neo-capillary created in the interstitial space. Capillary-2, between three cardiomyocytes (CMs), has a TC and Tps in the vicinity, but the distance between abluminal membrane of endothelium and TC/Tp plasma membrane is wider. Thus, capillary-2 is probably an ‘old’ capillary.
Mentions: Thirty days after the LADC ligation, a process of recovering characterizes the border zone of the myocardial infarction (Fig. 4). CMs begin to have normal ultrastructural appearance. In the interstitium, the number of TCs is clearly increased in comparison with normal myocardium. TCs/Tps have close spatial relationships with blood capillaries. However, the distances separating the Tps and abluminal front of endothelial cells and Tps are different for capillary of neo-formation (e.g. capillary-1 in Fig. 4) versus preexisting capillaries (e.g. capillary-2 in Fig. 4). Digitally measurements of the nanoscopic and microscopic distances resulted in the following results:

Bottom Line: Higher resolution images provided the following information: (a) the intercellular space between the abluminal face of endothelium and its surrounding TCs is frequently less than 50 nm; (b) TCs establish multiple direct nanocontacts with endothelial cells, where the extracellular space seems obliterated; such nanocontacts have a length of 0.4-1.5 μm; (c) the absence of basal membrane on the abluminal face of endothelial cell.Besides the physical contacts (either nanoscopic or microscopic) TCs presumably contribute to neo-angiognesis via paracrine secretion (as shown by immunocytochemistry for VEGF or NOS2).Last but not least, TCs contain measurable quantities of angiogenic microRNAs (e.g. let-7e, 10a, 21, 27b, 100, 126-3p, 130a, 143, 155, 503).

View Article: PubMed Central - PubMed

Affiliation: Department of Cellular and Molecular Medicine, Carol Davila University of Medicine and Pharmacy, Bucharest, Romania.

Show MeSH
Related in: MedlinePlus