Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures.
Bottom Line: On osteogenic induction, the level of matrix mineralization of ADSCs from group B was comparable to that of ADSCs from group A, whereas BMSCs from group B produced least amount of mineral deposits and had a lower expression level of osteogenic genes.The p21 gene expression and senescence-associated β-galactosidase activity were lower in ADSCs compared to BMSCs, which may be partly responsible for the greater proliferation and differentiation potential of ADSCs.It is concluded that the proliferation and osteogenic differentiation of ADSCs were less affected by age and multiple passage than BMSCs, suggesting that ADSCs may become a potentially effective therapeutic option for cell-based therapy, especially in elderly patients with osteoporosis.
Affiliation: Department of Fragrance and Cosmetic Science, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.Show MeSH
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Mentions: ADSC-A and ADSC-B were capable of achieving a cell number of 108 within 4 passages and 1013 within 7 passages (Fig. 3). BMSC-A and BMSC-B reached a cell number of 108 after 5 passages, but only BMSC-A attained to a population of 1013 at the 8th passage, while the accumulated cell number of BMSC-B levelled off at 109. Statistical analysis of the slope of the plots of accumulated cell numbers indicated that the growth rate of ADSC-A was comparable to that of ADSC-B, but the difference in cell accumulation between ADSC-B and BMSC-B was statistically significant (P = 0.038). The growth of BMSC-B was apparently delayed, although significant difference between BMSC-A and BMSC-B was not detected, due possibly to a low case number. Significant difference was not found when we compared the accumulated cell number of ADSCs and BMSCs by gender (data not shown).
Affiliation: Department of Fragrance and Cosmetic Science, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan.