Similar cation channels mediate protection from cerebellar exitotoxicity by exercise and inheritance.
Bottom Line: Sedentary FVB/N and exercised C57BL/6 mice both expressed higher levels of these cation channels compared to sedentary C57BL/6 mice, and were both found to be less sensitive to glutamate toxicity.In addition, our findings highlight the involvement of the cholinergic anti-inflammatory pathway in insult-inducible cerebellar processes.These mechanisms are likely to play similar roles in other brain regions and injuries as well, opening new venues for targeted research efforts.
Affiliation: Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel.Show MeSH
Related in: MedlinePlus
Mentions: First, we examined changes in Kir6.1 protein levels at a later time point. After the induction of Kir6.1 transcription 1 day post- kainate injection, Kir6.1 protein levels returned to those seen in control C57BL/6 mice within 14 days (n ∇ 6–9 per group, Fig. S3), reflecting the transient nature of the induced changes. The transient transcriptional induction by kainate could be either a marker of cell damage, or a beneficial protection mechanism. Therefore, we tested basal Kir6.1 protein levels in the more resistant, namely exercised C57BL/6 or sedentary FVB/N mice. Exercise significantly enhanced Kir6.1 labelling in BG of sedentary naïve mice. Similarly, in FVB/N, the basal levels of Kir6.1 were higher compared to those of C57BL/6 mice (n ∇ 6–9, P < 0.005, Student’s t-test, Fig. 3A), Thus, higher levels of Kir6.1 protein expression in BG were correlated with improved resistance to kainate in non-injected mice.
Affiliation: Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem, Israel.