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Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells.

Tudoran O, Soritau O, Balacescu O, Balacescu L, Braicu C, Rus M, Gherman C, Virag P, Irimie F, Berindan-Neagoe I - J. Cell. Mol. Med. (2012)

Bottom Line: In this study, we investigated the anti-angiogenic effects of EGCG treatment (10 μM) on human cervical tumour cells (HeLa) by evaluating the changes in the expression pattern of 84 genes known to be involved in the angiogenesis process.Transcriptional analysis revealed 11 genes to be differentially expressed and was further validated by measuring the induced biological effects.We observed reduced proliferation rates, adhesion and spreading ability as well as invasiveness of HeLa tumour cells upon treatment, which suggest that EGCG might be an important anti-angiogenic therapeutic approach in cervical cancers.

View Article: PubMed Central - PubMed

Affiliation: 'I. Chiricuta Cancer Institute, Department of Functional Genomics and Experimental Pathology, Cluj-Napoca, Romania. oana.tudoran@iocn.ro

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Adherent HeLa cells to collagen and laminin after 20 hrs of incubation. Twenty-four and forty-eight hours after treatment, cells were harvested, resuspended and seeded in a 96-well plate which was pre-treated with 15 μg/ml type IV collagen or laminin. Cells were allowed to adhere for 20 hrs and non-attached cells were removed by washing. Attached cells were fixed, stained with Giemsa solution, followed by measuring the absorbance at 630 nm.
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fig04: Adherent HeLa cells to collagen and laminin after 20 hrs of incubation. Twenty-four and forty-eight hours after treatment, cells were harvested, resuspended and seeded in a 96-well plate which was pre-treated with 15 μg/ml type IV collagen or laminin. Cells were allowed to adhere for 20 hrs and non-attached cells were removed by washing. Attached cells were fixed, stained with Giemsa solution, followed by measuring the absorbance at 630 nm.

Mentions: We evaluated the influence of EGCG on cellular adhesion and motility on type IV collagen and laminin coated plates (Figs 3 and 5), two major components of ECM. Our results show (Fig. 3) that after 48 hrs of EGCG treatment the attachment of HeLa cells to type IV collagen was stimulated, but had no effect on the adhesion to laminin. There were no differences in cells attachment observed at 24 hrs after treatment (data not shown). In contrast, if the cells were allowed to attach for 20 hrs, the adhesion was dramatically inhibited (Fig. 4). Untreated cells presented multiple filopodia and lamellipodia, characteristic for spreading cells, whereas most of the EGCG treated cells maintained their round shape even after 20 hrs, indicating reduced spreading ability (Fig. 5).


Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells.

Tudoran O, Soritau O, Balacescu O, Balacescu L, Braicu C, Rus M, Gherman C, Virag P, Irimie F, Berindan-Neagoe I - J. Cell. Mol. Med. (2012)

Adherent HeLa cells to collagen and laminin after 20 hrs of incubation. Twenty-four and forty-eight hours after treatment, cells were harvested, resuspended and seeded in a 96-well plate which was pre-treated with 15 μg/ml type IV collagen or laminin. Cells were allowed to adhere for 20 hrs and non-attached cells were removed by washing. Attached cells were fixed, stained with Giemsa solution, followed by measuring the absorbance at 630 nm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822928&req=5

fig04: Adherent HeLa cells to collagen and laminin after 20 hrs of incubation. Twenty-four and forty-eight hours after treatment, cells were harvested, resuspended and seeded in a 96-well plate which was pre-treated with 15 μg/ml type IV collagen or laminin. Cells were allowed to adhere for 20 hrs and non-attached cells were removed by washing. Attached cells were fixed, stained with Giemsa solution, followed by measuring the absorbance at 630 nm.
Mentions: We evaluated the influence of EGCG on cellular adhesion and motility on type IV collagen and laminin coated plates (Figs 3 and 5), two major components of ECM. Our results show (Fig. 3) that after 48 hrs of EGCG treatment the attachment of HeLa cells to type IV collagen was stimulated, but had no effect on the adhesion to laminin. There were no differences in cells attachment observed at 24 hrs after treatment (data not shown). In contrast, if the cells were allowed to attach for 20 hrs, the adhesion was dramatically inhibited (Fig. 4). Untreated cells presented multiple filopodia and lamellipodia, characteristic for spreading cells, whereas most of the EGCG treated cells maintained their round shape even after 20 hrs, indicating reduced spreading ability (Fig. 5).

Bottom Line: In this study, we investigated the anti-angiogenic effects of EGCG treatment (10 μM) on human cervical tumour cells (HeLa) by evaluating the changes in the expression pattern of 84 genes known to be involved in the angiogenesis process.Transcriptional analysis revealed 11 genes to be differentially expressed and was further validated by measuring the induced biological effects.We observed reduced proliferation rates, adhesion and spreading ability as well as invasiveness of HeLa tumour cells upon treatment, which suggest that EGCG might be an important anti-angiogenic therapeutic approach in cervical cancers.

View Article: PubMed Central - PubMed

Affiliation: 'I. Chiricuta Cancer Institute, Department of Functional Genomics and Experimental Pathology, Cluj-Napoca, Romania. oana.tudoran@iocn.ro

Show MeSH
Related in: MedlinePlus