Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells.
Bottom Line: In this study, we investigated the anti-angiogenic effects of EGCG treatment (10 μM) on human cervical tumour cells (HeLa) by evaluating the changes in the expression pattern of 84 genes known to be involved in the angiogenesis process.Transcriptional analysis revealed 11 genes to be differentially expressed and was further validated by measuring the induced biological effects.We observed reduced proliferation rates, adhesion and spreading ability as well as invasiveness of HeLa tumour cells upon treatment, which suggest that EGCG might be an important anti-angiogenic therapeutic approach in cervical cancers.
Affiliation: 'I. Chiricuta Cancer Institute, Department of Functional Genomics and Experimental Pathology, Cluj-Napoca, Romania. firstname.lastname@example.orgShow MeSH
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Mentions: We evaluated the influence of EGCG on cellular adhesion and motility on type IV collagen and laminin coated plates (Figs 3 and 5), two major components of ECM. Our results show (Fig. 3) that after 48 hrs of EGCG treatment the attachment of HeLa cells to type IV collagen was stimulated, but had no effect on the adhesion to laminin. There were no differences in cells attachment observed at 24 hrs after treatment (data not shown). In contrast, if the cells were allowed to attach for 20 hrs, the adhesion was dramatically inhibited (Fig. 4). Untreated cells presented multiple filopodia and lamellipodia, characteristic for spreading cells, whereas most of the EGCG treated cells maintained their round shape even after 20 hrs, indicating reduced spreading ability (Fig. 5).
Affiliation: 'I. Chiricuta Cancer Institute, Department of Functional Genomics and Experimental Pathology, Cluj-Napoca, Romania. email@example.com