Early transcriptional pattern of angiogenesis induced by EGCG treatment in cervical tumour cells.
Bottom Line: In this study, we investigated the anti-angiogenic effects of EGCG treatment (10 μM) on human cervical tumour cells (HeLa) by evaluating the changes in the expression pattern of 84 genes known to be involved in the angiogenesis process.Transcriptional analysis revealed 11 genes to be differentially expressed and was further validated by measuring the induced biological effects.Our results show that EGCG treatment not only leads to the down-regulation of genes involved in the stimulation of proliferation, adhesion and motility as well as invasion processes, but also to the up-regulation of several genes known to have antagonist effects.
Affiliation: 'I. Chiricuta Cancer Institute, Department of Functional Genomics and Experimental Pathology, Cluj-Napoca, Romania. email@example.comShow MeSH
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Mentions: We evaluated the influence of EGCG on cellular adhesion and motility on type IV collagen and laminin coated plates (Figs 3 and 5), two major components of ECM. Our results show (Fig. 3) that after 48 hrs of EGCG treatment the attachment of HeLa cells to type IV collagen was stimulated, but had no effect on the adhesion to laminin. There were no differences in cells attachment observed at 24 hrs after treatment (data not shown). In contrast, if the cells were allowed to attach for 20 hrs, the adhesion was dramatically inhibited (Fig. 4). Untreated cells presented multiple filopodia and lamellipodia, characteristic for spreading cells, whereas most of the EGCG treated cells maintained their round shape even after 20 hrs, indicating reduced spreading ability (Fig. 5).
Affiliation: 'I. Chiricuta Cancer Institute, Department of Functional Genomics and Experimental Pathology, Cluj-Napoca, Romania. firstname.lastname@example.org