Limits...
Regulatory mechanisms of interleukin-8 production induced by tumour necrosis factor-α in human hepatocellular carcinoma cells.

Wang Y, Wang W, Wang L, Wang X, Xia J - J. Cell. Mol. Med. (2012)

Bottom Line: NF-κB p65 protein nuclear translocation was determined by non-radioactive NF-κB p50/p65 transcription factor activity kit and cell bio-behaviours were detected by the real-time cell-monitoring system.In inflammatory micro-environment, HCC auto-produced IL-8 through p38 MAPK, ERK and PI3K/Akt signalling pathways, where the p38 MAPK is a central factor to activate the NF-κB pathway and regulate the expression of IL-8 production.There was a potential cross-talking between receptors.

View Article: PubMed Central - PubMed

Affiliation: Liver Cancer Institute, Shanghai Medical College, Fudan University, Shanghai, China.

Show MeSH

Related in: MedlinePlus

Bio-behaviours of hepatocellular cancer cells. Dynamic alterations in increased rate (%) of total cell number (A), stable cell number (B) and dead cell number (D) and decreased rate (%) of differentiated cell number (D) were measured by the real-time cell monitoring system, every 5 min. with 24 hrs after a 24 hrs culture of MHCC-97H cells pre-treated with vehicle (control), LY294002 (LY-5 μM), Wortmannin (WT-5 μM), SB203580 (SB-5 μM) and PD98059 (PD-5 μM) alone at the dose of 5 μM for 1 hr followed by the challenge with PBS or TNF-α at the dose of 1 ng/ml (TNF-α-1 ng/ml).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3822926&req=5

fig07: Bio-behaviours of hepatocellular cancer cells. Dynamic alterations in increased rate (%) of total cell number (A), stable cell number (B) and dead cell number (D) and decreased rate (%) of differentiated cell number (D) were measured by the real-time cell monitoring system, every 5 min. with 24 hrs after a 24 hrs culture of MHCC-97H cells pre-treated with vehicle (control), LY294002 (LY-5 μM), Wortmannin (WT-5 μM), SB203580 (SB-5 μM) and PD98059 (PD-5 μM) alone at the dose of 5 μM for 1 hr followed by the challenge with PBS or TNF-α at the dose of 1 ng/ml (TNF-α-1 ng/ml).

Mentions: Dynamic alternations of total, stable, differentiated and dead cell number were measured and recorded every 5 min. for 24 hrs after a 24 hrs pre-treatment with those inhibitors at 5 μM and TNF-α at 1 ng/ml, respectively, or 1 hr pre-treatment with those inhibitors 5 μM followed by the challenge with TNF-α at 1 ng/ml. The increased rate of total cell number was significantly higher at SB203580 alone and lower at PD98059 alone as compared with controls, whereas the decreased rate of differentiated cell number was conversed (P < 0.05 or 0.01, respectively, in Fig. S2). The increased rate of dead cell number in control was significantly higher than that with TNF-α or inhibitors, of which the group with LY294002 had significantly higher rate than that with TNF-α, Wortmmanin, SB203580 or PD98059 (Fig. 7). The increased rate of total cell number in groups with TNF-α and LY294002 or TNF-α and SB203580 was significantly higher than that in controls or TNF-α alone (P < 0.05 or 0.01, respectively), whereas the decreased rate of differentiated cell number showed opposite. The increased rates of stable or dead cell number in groups with TNF-α alone or TNF-α and PD98059 were higher. IL-8 induced a significant increase of cell proliferation at 48 and 72 hrs (Fig. S3), whereas TNF-α reduced cells proliferation from 24 hrs (Fig. S4).


Regulatory mechanisms of interleukin-8 production induced by tumour necrosis factor-α in human hepatocellular carcinoma cells.

Wang Y, Wang W, Wang L, Wang X, Xia J - J. Cell. Mol. Med. (2012)

Bio-behaviours of hepatocellular cancer cells. Dynamic alterations in increased rate (%) of total cell number (A), stable cell number (B) and dead cell number (D) and decreased rate (%) of differentiated cell number (D) were measured by the real-time cell monitoring system, every 5 min. with 24 hrs after a 24 hrs culture of MHCC-97H cells pre-treated with vehicle (control), LY294002 (LY-5 μM), Wortmannin (WT-5 μM), SB203580 (SB-5 μM) and PD98059 (PD-5 μM) alone at the dose of 5 μM for 1 hr followed by the challenge with PBS or TNF-α at the dose of 1 ng/ml (TNF-α-1 ng/ml).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822926&req=5

fig07: Bio-behaviours of hepatocellular cancer cells. Dynamic alterations in increased rate (%) of total cell number (A), stable cell number (B) and dead cell number (D) and decreased rate (%) of differentiated cell number (D) were measured by the real-time cell monitoring system, every 5 min. with 24 hrs after a 24 hrs culture of MHCC-97H cells pre-treated with vehicle (control), LY294002 (LY-5 μM), Wortmannin (WT-5 μM), SB203580 (SB-5 μM) and PD98059 (PD-5 μM) alone at the dose of 5 μM for 1 hr followed by the challenge with PBS or TNF-α at the dose of 1 ng/ml (TNF-α-1 ng/ml).
Mentions: Dynamic alternations of total, stable, differentiated and dead cell number were measured and recorded every 5 min. for 24 hrs after a 24 hrs pre-treatment with those inhibitors at 5 μM and TNF-α at 1 ng/ml, respectively, or 1 hr pre-treatment with those inhibitors 5 μM followed by the challenge with TNF-α at 1 ng/ml. The increased rate of total cell number was significantly higher at SB203580 alone and lower at PD98059 alone as compared with controls, whereas the decreased rate of differentiated cell number was conversed (P < 0.05 or 0.01, respectively, in Fig. S2). The increased rate of dead cell number in control was significantly higher than that with TNF-α or inhibitors, of which the group with LY294002 had significantly higher rate than that with TNF-α, Wortmmanin, SB203580 or PD98059 (Fig. 7). The increased rate of total cell number in groups with TNF-α and LY294002 or TNF-α and SB203580 was significantly higher than that in controls or TNF-α alone (P < 0.05 or 0.01, respectively), whereas the decreased rate of differentiated cell number showed opposite. The increased rates of stable or dead cell number in groups with TNF-α alone or TNF-α and PD98059 were higher. IL-8 induced a significant increase of cell proliferation at 48 and 72 hrs (Fig. S3), whereas TNF-α reduced cells proliferation from 24 hrs (Fig. S4).

Bottom Line: NF-κB p65 protein nuclear translocation was determined by non-radioactive NF-κB p50/p65 transcription factor activity kit and cell bio-behaviours were detected by the real-time cell-monitoring system.In inflammatory micro-environment, HCC auto-produced IL-8 through p38 MAPK, ERK and PI3K/Akt signalling pathways, where the p38 MAPK is a central factor to activate the NF-κB pathway and regulate the expression of IL-8 production.There was a potential cross-talking between receptors.

View Article: PubMed Central - PubMed

Affiliation: Liver Cancer Institute, Shanghai Medical College, Fudan University, Shanghai, China.

Show MeSH
Related in: MedlinePlus