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Cardiac shock wave therapy: assessment of safety and new insights into mechanisms of tissue regeneration.

Di Meglio F, Nurzynska D, Castaldo C, Miraglia R, Romano V, De Angelis A, Piegari E, Russo S, Montagnani S - J. Cell. Mol. Med. (2012)

Bottom Line: ECSW application did not provoke arrhythmia or increase the troponin-I level.At all time points, the left ventricular ejection fraction and fractional shortening remained stable.In conclusion, non-invasive ECSW therapy is a safe and effective way of activating cardiac stem cells and myocardial regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomorphological and Functional Sciences, University of Naples Federico II, Naples, Italy.

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ECSW application does not induce structural damage to the heart. Histochemistry and immunohistochemistry methods were used to evaluate the morphology of cardiac tissue in control and treated animals at 3 months after ECSW application. Haematoxylin and eosin staining revealed no signs of inflammatory infiltration in control (A) or SW-treated hearts (B). Masson’s trichrome (collagen in blue; C, D) and Picrosirius stainings (collagen in red; E, F) revealed no signs of fibrosis in control (C, E) or SW-treated hearts (D, F). Scale bar: 100 μm.
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fig02: ECSW application does not induce structural damage to the heart. Histochemistry and immunohistochemistry methods were used to evaluate the morphology of cardiac tissue in control and treated animals at 3 months after ECSW application. Haematoxylin and eosin staining revealed no signs of inflammatory infiltration in control (A) or SW-treated hearts (B). Masson’s trichrome (collagen in blue; C, D) and Picrosirius stainings (collagen in red; E, F) revealed no signs of fibrosis in control (C, E) or SW-treated hearts (D, F). Scale bar: 100 μm.

Mentions: Collagen plays a vital role in maintaining structural integrity and in determining tissue function. In the heart, both decreases in [16] and excess accumulations of collagen [17] can be detrimental. We used Masson’s trichrome and Picrosirius red stains (Fig. 2C–F) to visualize the fine collagen fibres in the myocardium of control and ECSW-treated animals. Neither control nor ECSW-treated animals manifested cardiac fibrosis throughout the study period. The myocardial collagen content was similar in control and treated animals up to 3 months after ECSW treatment. In terms of possible endothelial damage, there was no thickening of the blood vessel intima. Local subepicardial vasculogenesis was observed in treated rats in regions corresponding to the transthoracic SW application zone; however, inflammatory interstitial infiltration was not observed in the cardiac tissue of treated animals (Fig. 2A and B).


Cardiac shock wave therapy: assessment of safety and new insights into mechanisms of tissue regeneration.

Di Meglio F, Nurzynska D, Castaldo C, Miraglia R, Romano V, De Angelis A, Piegari E, Russo S, Montagnani S - J. Cell. Mol. Med. (2012)

ECSW application does not induce structural damage to the heart. Histochemistry and immunohistochemistry methods were used to evaluate the morphology of cardiac tissue in control and treated animals at 3 months after ECSW application. Haematoxylin and eosin staining revealed no signs of inflammatory infiltration in control (A) or SW-treated hearts (B). Masson’s trichrome (collagen in blue; C, D) and Picrosirius stainings (collagen in red; E, F) revealed no signs of fibrosis in control (C, E) or SW-treated hearts (D, F). Scale bar: 100 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822862&req=5

fig02: ECSW application does not induce structural damage to the heart. Histochemistry and immunohistochemistry methods were used to evaluate the morphology of cardiac tissue in control and treated animals at 3 months after ECSW application. Haematoxylin and eosin staining revealed no signs of inflammatory infiltration in control (A) or SW-treated hearts (B). Masson’s trichrome (collagen in blue; C, D) and Picrosirius stainings (collagen in red; E, F) revealed no signs of fibrosis in control (C, E) or SW-treated hearts (D, F). Scale bar: 100 μm.
Mentions: Collagen plays a vital role in maintaining structural integrity and in determining tissue function. In the heart, both decreases in [16] and excess accumulations of collagen [17] can be detrimental. We used Masson’s trichrome and Picrosirius red stains (Fig. 2C–F) to visualize the fine collagen fibres in the myocardium of control and ECSW-treated animals. Neither control nor ECSW-treated animals manifested cardiac fibrosis throughout the study period. The myocardial collagen content was similar in control and treated animals up to 3 months after ECSW treatment. In terms of possible endothelial damage, there was no thickening of the blood vessel intima. Local subepicardial vasculogenesis was observed in treated rats in regions corresponding to the transthoracic SW application zone; however, inflammatory interstitial infiltration was not observed in the cardiac tissue of treated animals (Fig. 2A and B).

Bottom Line: ECSW application did not provoke arrhythmia or increase the troponin-I level.At all time points, the left ventricular ejection fraction and fractional shortening remained stable.In conclusion, non-invasive ECSW therapy is a safe and effective way of activating cardiac stem cells and myocardial regeneration.

View Article: PubMed Central - PubMed

Affiliation: Department of Biomorphological and Functional Sciences, University of Naples Federico II, Naples, Italy.

Show MeSH
Related in: MedlinePlus