C-peptide promotes lesion development in a mouse model of arteriosclerosis.
Bottom Line: Moreover, lesions of C-peptide-treated mice contained significantly more macrophages (1.6 ± 0.3% versus 0.7 ± 0.2% positive area; P < 0.01) and more vascular smooth muscle cells (4.8 ± 0.6% versus 2.4 ± 0.3% positive area; P < 0.01).Finally, lipid deposition measured by Oil-red-O staining in the aortic arch was significantly higher in the C-peptide group compared with controls.Our results demonstrate that elevated C-peptide levels promote inflammatory cell infiltration and lesion development in ApoE-deficient mice without having metabolic effects.
Affiliation: Department of Internal Medicine II - Cardiology, University of Ulm, Ulm, Germany.Show MeSH
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Mentions: Finally, we investigated lipid deposition in lesions using Oil-red-O staining. In C-peptide–treated mice, lipid deposition in the aortic arch was significantly higher compared with controls (14.8 ± 1.5 versus 9.5 ± 1.6; P < 0.05; Fig. 6A). Moreover, there was a trend—albeit not significant—towards increased lipid deposition in en face preparations of the abdominal and thoracic aorta in C-peptide–treated mice compared to control mice (Fig. 6B).
Affiliation: Department of Internal Medicine II - Cardiology, University of Ulm, Ulm, Germany.