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C-peptide promotes lesion development in a mouse model of arteriosclerosis.

Vasic D, Marx N, Sukhova G, Bach H, Durst R, Grüb M, Hausauer A, Hombach V, Rottbauer W, Walcher D - J. Cell. Mol. Med. (2012)

Bottom Line: C-peptide treatment significantly increased C-peptide blood levels by 4.8-fold without having an effect on glucose or insulin levels, nor on the lipid profile.Finally, lipid deposition measured by Oil-red-O staining in the aortic arch was significantly higher in the C-peptide group compared with controls.Our results demonstrate that elevated C-peptide levels promote inflammatory cell infiltration and lesion development in ApoE-deficient mice without having metabolic effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine II - Cardiology, University of Ulm, Ulm, Germany.

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(A) C-peptide treatment increases lipid content in the aortic arch. Upper: representative Oil-red-O stained sections of the aortic arch show lipid deposition in a placebo (a) and a C-peptide–treated mouse (b). Insert: high power view of the area indicated by the rectangle in (b). Lower: Quantitative image analysis of Oil-red-O positivity in the aortic arch expressed as % positive area. ApoE-deficient mice on chow or high fat diet alone served as controls. Each data point represents a value from a single mouse (chow diet: n= 5, high fat diet: n= 8, placebo: n= 17, C-peptide: n= 18); *P < 0.05 for comparison between groups. (B) C-peptide treatment increases plaque formation in mice. Left: Representative microphotographs of thoracic and abdominal aortic specimen stained for lipid deposition with Oil-red-O in a control (a) and a C-peptide–treated mouse (b). Arrows indicate fat rich plaques. Right: Quantitative image analysis of Oil-red-O positivity in the en face aorta expressed as % positive area. ApoE-deficient mice on chow or high fat diet alone served as controls. Each data point represents a value from a single mouse (chow diet: n= 5, high fat diet: n= 8, placebo: n= 17, C-peptide: n= 18); P is not significant for comparison between groups.
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fig06: (A) C-peptide treatment increases lipid content in the aortic arch. Upper: representative Oil-red-O stained sections of the aortic arch show lipid deposition in a placebo (a) and a C-peptide–treated mouse (b). Insert: high power view of the area indicated by the rectangle in (b). Lower: Quantitative image analysis of Oil-red-O positivity in the aortic arch expressed as % positive area. ApoE-deficient mice on chow or high fat diet alone served as controls. Each data point represents a value from a single mouse (chow diet: n= 5, high fat diet: n= 8, placebo: n= 17, C-peptide: n= 18); *P < 0.05 for comparison between groups. (B) C-peptide treatment increases plaque formation in mice. Left: Representative microphotographs of thoracic and abdominal aortic specimen stained for lipid deposition with Oil-red-O in a control (a) and a C-peptide–treated mouse (b). Arrows indicate fat rich plaques. Right: Quantitative image analysis of Oil-red-O positivity in the en face aorta expressed as % positive area. ApoE-deficient mice on chow or high fat diet alone served as controls. Each data point represents a value from a single mouse (chow diet: n= 5, high fat diet: n= 8, placebo: n= 17, C-peptide: n= 18); P is not significant for comparison between groups.

Mentions: Finally, we investigated lipid deposition in lesions using Oil-red-O staining. In C-peptide–treated mice, lipid deposition in the aortic arch was significantly higher compared with controls (14.8 ± 1.5 versus 9.5 ± 1.6; P < 0.05; Fig. 6A). Moreover, there was a trend—albeit not significant—towards increased lipid deposition in en face preparations of the abdominal and thoracic aorta in C-peptide–treated mice compared to control mice (Fig. 6B).


C-peptide promotes lesion development in a mouse model of arteriosclerosis.

Vasic D, Marx N, Sukhova G, Bach H, Durst R, Grüb M, Hausauer A, Hombach V, Rottbauer W, Walcher D - J. Cell. Mol. Med. (2012)

(A) C-peptide treatment increases lipid content in the aortic arch. Upper: representative Oil-red-O stained sections of the aortic arch show lipid deposition in a placebo (a) and a C-peptide–treated mouse (b). Insert: high power view of the area indicated by the rectangle in (b). Lower: Quantitative image analysis of Oil-red-O positivity in the aortic arch expressed as % positive area. ApoE-deficient mice on chow or high fat diet alone served as controls. Each data point represents a value from a single mouse (chow diet: n= 5, high fat diet: n= 8, placebo: n= 17, C-peptide: n= 18); *P < 0.05 for comparison between groups. (B) C-peptide treatment increases plaque formation in mice. Left: Representative microphotographs of thoracic and abdominal aortic specimen stained for lipid deposition with Oil-red-O in a control (a) and a C-peptide–treated mouse (b). Arrows indicate fat rich plaques. Right: Quantitative image analysis of Oil-red-O positivity in the en face aorta expressed as % positive area. ApoE-deficient mice on chow or high fat diet alone served as controls. Each data point represents a value from a single mouse (chow diet: n= 5, high fat diet: n= 8, placebo: n= 17, C-peptide: n= 18); P is not significant for comparison between groups.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3822861&req=5

fig06: (A) C-peptide treatment increases lipid content in the aortic arch. Upper: representative Oil-red-O stained sections of the aortic arch show lipid deposition in a placebo (a) and a C-peptide–treated mouse (b). Insert: high power view of the area indicated by the rectangle in (b). Lower: Quantitative image analysis of Oil-red-O positivity in the aortic arch expressed as % positive area. ApoE-deficient mice on chow or high fat diet alone served as controls. Each data point represents a value from a single mouse (chow diet: n= 5, high fat diet: n= 8, placebo: n= 17, C-peptide: n= 18); *P < 0.05 for comparison between groups. (B) C-peptide treatment increases plaque formation in mice. Left: Representative microphotographs of thoracic and abdominal aortic specimen stained for lipid deposition with Oil-red-O in a control (a) and a C-peptide–treated mouse (b). Arrows indicate fat rich plaques. Right: Quantitative image analysis of Oil-red-O positivity in the en face aorta expressed as % positive area. ApoE-deficient mice on chow or high fat diet alone served as controls. Each data point represents a value from a single mouse (chow diet: n= 5, high fat diet: n= 8, placebo: n= 17, C-peptide: n= 18); P is not significant for comparison between groups.
Mentions: Finally, we investigated lipid deposition in lesions using Oil-red-O staining. In C-peptide–treated mice, lipid deposition in the aortic arch was significantly higher compared with controls (14.8 ± 1.5 versus 9.5 ± 1.6; P < 0.05; Fig. 6A). Moreover, there was a trend—albeit not significant—towards increased lipid deposition in en face preparations of the abdominal and thoracic aorta in C-peptide–treated mice compared to control mice (Fig. 6B).

Bottom Line: C-peptide treatment significantly increased C-peptide blood levels by 4.8-fold without having an effect on glucose or insulin levels, nor on the lipid profile.Finally, lipid deposition measured by Oil-red-O staining in the aortic arch was significantly higher in the C-peptide group compared with controls.Our results demonstrate that elevated C-peptide levels promote inflammatory cell infiltration and lesion development in ApoE-deficient mice without having metabolic effects.

View Article: PubMed Central - PubMed

Affiliation: Department of Internal Medicine II - Cardiology, University of Ulm, Ulm, Germany.

Show MeSH
Related in: MedlinePlus