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Caveolin induces cardioprotection through epigenetic regulation.

Das M, Das S, Lekli I, Das DK - J. Cell. Mol. Med. (2012)

Bottom Line: Cav-1 KO mouse also decreased the translocation of forkhead transcription factor (FOXO3a) to the nucleus and reduced the induction of the expression of SIRT-1 in the preconditioned heart.Cardioprotective property of Cav-1 was further confirmed by reduced ventricular function, increased cardiomyocyte apoptosis, increased expression of junas kinase (JNK) and Bax and decreased expression of phospho-adenosine monophosphate-activated protein kinase (AMPK), phospho-AKT and B cell lymphoma-2 (Bcl-2) in Cav-1 KO preconditioned heart.The results clearly indicate that Cav-1 induces cardioprotection through epigenetic regulation.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Research Center, University of Connecticut School of Medicine, Farmington, CT 06-030-1110, USA.

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Related in: MedlinePlus

Effect of caveolin-1 on caspase-3 and cytochrome c. C: control; I/R: ischaemia/reperfusion; PC: precondition; WT: wild-type mouse; Cav-1: caveolin-1 knockout mouse. (A) Representative picture of Western blot analysis. (B) Densitometric scanning of the blots. Results are average ± S.E.M. of three different sets of blots. *P < 0.05 versus corresponding bots of Cav-1 KO.
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fig02: Effect of caveolin-1 on caspase-3 and cytochrome c. C: control; I/R: ischaemia/reperfusion; PC: precondition; WT: wild-type mouse; Cav-1: caveolin-1 knockout mouse. (A) Representative picture of Western blot analysis. (B) Densitometric scanning of the blots. Results are average ± S.E.M. of three different sets of blots. *P < 0.05 versus corresponding bots of Cav-1 KO.

Mentions: To study the level of apoptosis in Cav-1 KO mouse heart, caspase-3 and cytochrome c levels were examined by Western blot analysis. We have found that the level of full length caspase-3 is high in WT PC hearts compared Cav-1 I/R, Cav-1 PC and WT I/R heart. The level of cleaved caspase-3 was very low in WT PC heart compared to the other group of the heart. In case of cytochrome c, we tested the level of cytochrome c in cytosolic fractions of the heart and found that the level of myocardial cytochrome c was significantly higher in cytosolic fraction in WT I/R, Cav-1 KO I/R as well as in the Cav-1 KO PC heart compared to the WT PC group (Fig. 2).


Caveolin induces cardioprotection through epigenetic regulation.

Das M, Das S, Lekli I, Das DK - J. Cell. Mol. Med. (2012)

Effect of caveolin-1 on caspase-3 and cytochrome c. C: control; I/R: ischaemia/reperfusion; PC: precondition; WT: wild-type mouse; Cav-1: caveolin-1 knockout mouse. (A) Representative picture of Western blot analysis. (B) Densitometric scanning of the blots. Results are average ± S.E.M. of three different sets of blots. *P < 0.05 versus corresponding bots of Cav-1 KO.
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822857&req=5

fig02: Effect of caveolin-1 on caspase-3 and cytochrome c. C: control; I/R: ischaemia/reperfusion; PC: precondition; WT: wild-type mouse; Cav-1: caveolin-1 knockout mouse. (A) Representative picture of Western blot analysis. (B) Densitometric scanning of the blots. Results are average ± S.E.M. of three different sets of blots. *P < 0.05 versus corresponding bots of Cav-1 KO.
Mentions: To study the level of apoptosis in Cav-1 KO mouse heart, caspase-3 and cytochrome c levels were examined by Western blot analysis. We have found that the level of full length caspase-3 is high in WT PC hearts compared Cav-1 I/R, Cav-1 PC and WT I/R heart. The level of cleaved caspase-3 was very low in WT PC heart compared to the other group of the heart. In case of cytochrome c, we tested the level of cytochrome c in cytosolic fractions of the heart and found that the level of myocardial cytochrome c was significantly higher in cytosolic fraction in WT I/R, Cav-1 KO I/R as well as in the Cav-1 KO PC heart compared to the WT PC group (Fig. 2).

Bottom Line: Cav-1 KO mouse also decreased the translocation of forkhead transcription factor (FOXO3a) to the nucleus and reduced the induction of the expression of SIRT-1 in the preconditioned heart.Cardioprotective property of Cav-1 was further confirmed by reduced ventricular function, increased cardiomyocyte apoptosis, increased expression of junas kinase (JNK) and Bax and decreased expression of phospho-adenosine monophosphate-activated protein kinase (AMPK), phospho-AKT and B cell lymphoma-2 (Bcl-2) in Cav-1 KO preconditioned heart.The results clearly indicate that Cav-1 induces cardioprotection through epigenetic regulation.

View Article: PubMed Central - PubMed

Affiliation: Cardiovascular Research Center, University of Connecticut School of Medicine, Farmington, CT 06-030-1110, USA.

Show MeSH
Related in: MedlinePlus