Caveolin induces cardioprotection through epigenetic regulation.
Bottom Line: Cav-1 KO mouse also decreased the translocation of forkhead transcription factor (FOXO3a) to the nucleus and reduced the induction of the expression of SIRT-1 in the preconditioned heart.Cardioprotective property of Cav-1 was further confirmed by reduced ventricular function, increased cardiomyocyte apoptosis, increased expression of junas kinase (JNK) and Bax and decreased expression of phospho-adenosine monophosphate-activated protein kinase (AMPK), phospho-AKT and B cell lymphoma-2 (Bcl-2) in Cav-1 KO preconditioned heart.The results clearly indicate that Cav-1 induces cardioprotection through epigenetic regulation.
Affiliation: Cardiovascular Research Center, University of Connecticut School of Medicine, Farmington, CT 06-030-1110, USA.Show MeSH
Related in: MedlinePlus
Mentions: To study the level of apoptosis in Cav-1 KO mouse heart, caspase-3 and cytochrome c levels were examined by Western blot analysis. We have found that the level of full length caspase-3 is high in WT PC hearts compared Cav-1 I/R, Cav-1 PC and WT I/R heart. The level of cleaved caspase-3 was very low in WT PC heart compared to the other group of the heart. In case of cytochrome c, we tested the level of cytochrome c in cytosolic fractions of the heart and found that the level of myocardial cytochrome c was significantly higher in cytosolic fraction in WT I/R, Cav-1 KO I/R as well as in the Cav-1 KO PC heart compared to the WT PC group (Fig. 2).
Affiliation: Cardiovascular Research Center, University of Connecticut School of Medicine, Farmington, CT 06-030-1110, USA.