Anti-inflammatory potential of allyl-isothiocyanate--role of Nrf2, NF-(κ) B and microRNA-155.
Bottom Line: AITC decreased nuclear p65 protein levels, a subunit of the transcription factor NF-κB.Importantly, our data indicate that AITC significantly attenuated microRNA-155 levels in LPS-stimulated RAW264.7 macrophages in a dose-dependent manner.The anti-inflammatory effects of AITC were accompanied by an increase in Nrf2 nuclear translocation and consequently by an increase of mRNA and protein levels of the Nrf2 target gene heme-oxygenase 1.
Affiliation: Institute of Human Nutrition and Food Science, Christian-Albrechts-University, Kiel, Germany. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: No significant changes in feed intake (1.89 ± 0.46 versus 1.61 ± 0.57 g/d) and body weight gain (2.04 ± 0.57 versus 1.87 ± 0.48 g) between control and AITC-treated mice were observed during the study period. A 7-day-oral-application of AITC in mice fed a high-fat diet resulted in an increase in nuclear Nrf2 protein levels in the liver (Fig. 7C). This increase in nuclear Nrf2 protein was accompanied by a significant increase in Nrf2 and HO-1 mRNA levels (Fig. 7A and B). Administration of AITC resulted also in a moderate but not significant down-regulation of IL-1β, iNOS and miR-155 levels (Fig. 8A–C) in mouse liver. TNF-α mRNA levels were not affected by AITC treatment (data not shown). In an independent experiment, mice were fed either a low-fat standard or a high-fat diet for 8 weeks resulting in an increase of IL-1β liver mRNA levels in the high-fat diet as compared to the low-fat standard diet group. This observation is in line with literature data [24, 25], indicating an induction of pro-inflammatory markers in mice consuming a high-fat diet.
Affiliation: Institute of Human Nutrition and Food Science, Christian-Albrechts-University, Kiel, Germany. email@example.com