Anti-inflammatory potential of allyl-isothiocyanate--role of Nrf2, NF-(κ) B and microRNA-155.
Bottom Line: AITC decreased nuclear p65 protein levels, a subunit of the transcription factor NF-κB.Importantly, our data indicate that AITC significantly attenuated microRNA-155 levels in LPS-stimulated RAW264.7 macrophages in a dose-dependent manner.The anti-inflammatory effects of AITC were accompanied by an increase in Nrf2 nuclear translocation and consequently by an increase of mRNA and protein levels of the Nrf2 target gene heme-oxygenase 1.
Affiliation: Institute of Human Nutrition and Food Science, Christian-Albrechts-University, Kiel, Germany. firstname.lastname@example.orgShow MeSH
Related in: MedlinePlus
Mentions: A 6-hrs treatment of RAW264.7 cells with 10 ng/ml LPS caused an increase of p65-protein in the nucleus. This increase was lowered following incubation with 10 μmol/l AITC and SFN, respectively (Fig. 3).
Affiliation: Institute of Human Nutrition and Food Science, Christian-Albrechts-University, Kiel, Germany. email@example.com