Limits...
Anti-inflammatory potential of allyl-isothiocyanate--role of Nrf2, NF-(κ) B and microRNA-155.

Wagner AE, Boesch-Saadatmandi C, Dose J, Schultheiss G, Rimbach G - J. Cell. Mol. Med. (2012)

Bottom Line: AITC decreased nuclear p65 protein levels, a subunit of the transcription factor NF-κB.Importantly, our data indicate that AITC significantly attenuated microRNA-155 levels in LPS-stimulated RAW264.7 macrophages in a dose-dependent manner.The anti-inflammatory effects of AITC were accompanied by an increase in Nrf2 nuclear translocation and consequently by an increase of mRNA and protein levels of the Nrf2 target gene heme-oxygenase 1.

View Article: PubMed Central - PubMed

Affiliation: Institute of Human Nutrition and Food Science, Christian-Albrechts-University, Kiel, Germany. wagner@molecularnutrition.uni-kiel.de

Show MeSH

Related in: MedlinePlus

Effect of allyl-isothiocyanate (AITC) and sulforaphane (SFN) on NF-κB nuclear translocation. Protein levels of p65, a subunit of the transcription factor NF-κB, were measured by Western blotting in nucleic extracts from RAW264.7 macrophages incubated with the test compounds (10 μmol/l) and stimulated with LPS (10 ng/ml) for six hrs. One representative Western blot is shown.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3822852&req=5

fig03: Effect of allyl-isothiocyanate (AITC) and sulforaphane (SFN) on NF-κB nuclear translocation. Protein levels of p65, a subunit of the transcription factor NF-κB, were measured by Western blotting in nucleic extracts from RAW264.7 macrophages incubated with the test compounds (10 μmol/l) and stimulated with LPS (10 ng/ml) for six hrs. One representative Western blot is shown.

Mentions: A 6-hrs treatment of RAW264.7 cells with 10 ng/ml LPS caused an increase of p65-protein in the nucleus. This increase was lowered following incubation with 10 μmol/l AITC and SFN, respectively (Fig. 3).


Anti-inflammatory potential of allyl-isothiocyanate--role of Nrf2, NF-(κ) B and microRNA-155.

Wagner AE, Boesch-Saadatmandi C, Dose J, Schultheiss G, Rimbach G - J. Cell. Mol. Med. (2012)

Effect of allyl-isothiocyanate (AITC) and sulforaphane (SFN) on NF-κB nuclear translocation. Protein levels of p65, a subunit of the transcription factor NF-κB, were measured by Western blotting in nucleic extracts from RAW264.7 macrophages incubated with the test compounds (10 μmol/l) and stimulated with LPS (10 ng/ml) for six hrs. One representative Western blot is shown.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822852&req=5

fig03: Effect of allyl-isothiocyanate (AITC) and sulforaphane (SFN) on NF-κB nuclear translocation. Protein levels of p65, a subunit of the transcription factor NF-κB, were measured by Western blotting in nucleic extracts from RAW264.7 macrophages incubated with the test compounds (10 μmol/l) and stimulated with LPS (10 ng/ml) for six hrs. One representative Western blot is shown.
Mentions: A 6-hrs treatment of RAW264.7 cells with 10 ng/ml LPS caused an increase of p65-protein in the nucleus. This increase was lowered following incubation with 10 μmol/l AITC and SFN, respectively (Fig. 3).

Bottom Line: AITC decreased nuclear p65 protein levels, a subunit of the transcription factor NF-κB.Importantly, our data indicate that AITC significantly attenuated microRNA-155 levels in LPS-stimulated RAW264.7 macrophages in a dose-dependent manner.The anti-inflammatory effects of AITC were accompanied by an increase in Nrf2 nuclear translocation and consequently by an increase of mRNA and protein levels of the Nrf2 target gene heme-oxygenase 1.

View Article: PubMed Central - PubMed

Affiliation: Institute of Human Nutrition and Food Science, Christian-Albrechts-University, Kiel, Germany. wagner@molecularnutrition.uni-kiel.de

Show MeSH
Related in: MedlinePlus