Profilin1 facilitates staurosporine-triggered apoptosis by stabilizing the integrin β1-actin complex in breast cancer cells.
Bottom Line: In this study, we found that stable expression of ectopic Pfn1 sensitized the breast cancer cell line MDA-MB-468 to apoptosis induced by staurosporine, a widely used natural apoptosis-inducing agent.These results suggest that the insufficient fibronectin caused by the integrin α5β1 up-regulation might activate a signalling pathway leading to an increase of cellular apoptosis.Our study indicated a previously uncharacterized role of Pfn1 in mediating staurosporine-inducing apoptosis in breast cancer cells via up-regulating integrin α5β1, and suggested a new target for breast cancer therapy.
Affiliation: Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, China.Show MeSH
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Mentions: In conclusion, this study have clearly demonstrated how Pfn1 facilitates the STS-induced apoptosis by integrin and actin complex (Fig. 7) and provided new insight into the still ill-defined role of Pfn1 in the control of breast cancer cells responsiveness to chemotherapy. The use of Pfn1 and STS may be an important clinical consideration in treating breast cancer harbouring low integrin expression. The combination of Pfn1 and STS with clinically relevant medications deserves further investigations in vitro and in vivo, in view of the fact that there is still ongoing debate concerning the relevant mechanisms.
Affiliation: Department of Biochemistry and Molecular Biology, Shanghai Medical College, Fudan University, Shanghai, China.