Role of Slug transcription factor in human mesenchymal stem cells.
Bottom Line: In this study we found that Slug-silenced cells changed in morphology, decreased in their migration ability, increased Sox9 and Sox5 and decreased Sox6 and STAT1 expression.On the contrary, the effect of Slug depletion on Runx2 was influenced by cell type.Interestingly, we demonstrated a direct in vivo regulatory action of Slug by chromatin immunoprecipitation, showing a specific recruitment of this TF in the promoter of Runx2 and Sox9 genes.
Affiliation: Dipartimento di Biochimica e Biologia Molecolare, Sezione di Biologia Molecolare, Università degli Studi di Ferrara, Ferrara, Italy.Show MeSH
Related in: MedlinePlus
Mentions: Specifically, hMSCs were transfected with siRNA sequence targeting Slug gene transcript . Non-targeting siRNA was used as a negative control. We first checked that siRNA/Slug was efficient in decreasing Slug expression in all hMSCs. Quantitative real-time RT-PCR showed that basal Slug expression levels are higher in hTP- and hIC-MSCs than in hWJ-MSCs (Fig. 2A). Nevertheless, in all cases siRNA/Slug reduced Slug mRNA expression by about 80% (P < 0.05). The efficiency of Slug silencing was also validated at protein level in all hMSCs by Western blot analysis (Fig. 2B).
Affiliation: Dipartimento di Biochimica e Biologia Molecolare, Sezione di Biologia Molecolare, Università degli Studi di Ferrara, Ferrara, Italy.