Regulation of the (pro)renin-renin receptor in cardiac remodelling.
Bottom Line: Because none of the tested commercially available antibodies appeared to detect endogenous (P)RR, a (P)RR-specific polyclonal antibody was generated to study (P)RR protein levels. (P)RR protein levels were significantly increased in the post-MI rat heart (1.4-fold, P < 0.05) as compared to controls.Most interestingly in DCM patients, a significant 8.7-fold (P < 0.05) increase was observed.Thus, protein expression paralleled gene expression.
Affiliation: University Medical Center Groningen, Thorax Center, Department of Cardiology, University of Groningen, The Netherlands.Show MeSH
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Mentions: To elucidate the regulation of (P)RR in human heart failure, we first evaluated (P)RR expression of heart tissue from patients with DCM. (P)RR mRNA levels were up-regulated significantly about 5.3-fold (P < 0.001) in the hearts of DCM patients compared to healthy controls (Fig. 6A). In addition, protein levels of (P)RR showed a similar up-regulation and was about 8.8-fold (P < 0.05) higher in DCM patients compared to healthy controls (Fig. 6B and C).
Affiliation: University Medical Center Groningen, Thorax Center, Department of Cardiology, University of Groningen, The Netherlands.