Regulation of the (pro)renin-renin receptor in cardiac remodelling.
Bottom Line: Because none of the tested commercially available antibodies appeared to detect endogenous (P)RR, a (P)RR-specific polyclonal antibody was generated to study (P)RR protein levels. (P)RR protein levels were significantly increased in the post-MI rat heart (1.4-fold, P < 0.05) as compared to controls.Most interestingly in DCM patients, a significant 8.7-fold (P < 0.05) increase was observed.Thus, protein expression paralleled gene expression.
Affiliation: University Medical Center Groningen, Thorax Center, Department of Cardiology, University of Groningen, The Netherlands.Show MeSH
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Mentions: Having established (P)RR expression in the heart, we next investigated (P)RR expression in other organs. At the mRNA level, maximal (P)RR expression was observed in brain, followed by kidney, intestine, liver and heart (Fig. 3A). In muscle and lung, expression was nearly absent. At the protein level, maximal expression was observed in kidney followed by liver, brain, heart and intestine (Fig. 3B and C). Again, expression was low in muscle and lung. Except for brain, mRNA and protein expression levels paralleled each other.
Affiliation: University Medical Center Groningen, Thorax Center, Department of Cardiology, University of Groningen, The Netherlands.