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Regulation of the (pro)renin-renin receptor in cardiac remodelling.

Mahmud H, Silljé HH, Cannon MV, van Gilst WH, de Boer RA - J. Cell. Mol. Med. (2012)

Bottom Line: Because none of the tested commercially available antibodies appeared to detect endogenous (P)RR, a (P)RR-specific polyclonal antibody was generated to study (P)RR protein levels. (P)RR protein levels were significantly increased in the post-MI rat heart (1.4-fold, P < 0.05) as compared to controls.Most interestingly in DCM patients, a significant 8.7-fold (P < 0.05) increase was observed.Thus, protein expression paralleled gene expression.

View Article: PubMed Central - PubMed

Affiliation: University Medical Center Groningen, Thorax Center, Department of Cardiology, University of Groningen, The Netherlands.

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Expression of (P)RR mRNA and protein in different organs of C57Bl6/J mouse. (P)RR mRNA is highly expressed in brain, kidney, intestine and liver and moderate in heart and undetectable in skeletal muscle and lung (n = 4, A). (P)RR protein is highly expressed in kidney, brain, liver and intestine, moderate in heart and undetectable in skeletal muscle and lung (n = 4, B and C).
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fig03: Expression of (P)RR mRNA and protein in different organs of C57Bl6/J mouse. (P)RR mRNA is highly expressed in brain, kidney, intestine and liver and moderate in heart and undetectable in skeletal muscle and lung (n = 4, A). (P)RR protein is highly expressed in kidney, brain, liver and intestine, moderate in heart and undetectable in skeletal muscle and lung (n = 4, B and C).

Mentions: Having established (P)RR expression in the heart, we next investigated (P)RR expression in other organs. At the mRNA level, maximal (P)RR expression was observed in brain, followed by kidney, intestine, liver and heart (Fig. 3A). In muscle and lung, expression was nearly absent. At the protein level, maximal expression was observed in kidney followed by liver, brain, heart and intestine (Fig. 3B and C). Again, expression was low in muscle and lung. Except for brain, mRNA and protein expression levels paralleled each other.


Regulation of the (pro)renin-renin receptor in cardiac remodelling.

Mahmud H, Silljé HH, Cannon MV, van Gilst WH, de Boer RA - J. Cell. Mol. Med. (2012)

Expression of (P)RR mRNA and protein in different organs of C57Bl6/J mouse. (P)RR mRNA is highly expressed in brain, kidney, intestine and liver and moderate in heart and undetectable in skeletal muscle and lung (n = 4, A). (P)RR protein is highly expressed in kidney, brain, liver and intestine, moderate in heart and undetectable in skeletal muscle and lung (n = 4, B and C).
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Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822843&req=5

fig03: Expression of (P)RR mRNA and protein in different organs of C57Bl6/J mouse. (P)RR mRNA is highly expressed in brain, kidney, intestine and liver and moderate in heart and undetectable in skeletal muscle and lung (n = 4, A). (P)RR protein is highly expressed in kidney, brain, liver and intestine, moderate in heart and undetectable in skeletal muscle and lung (n = 4, B and C).
Mentions: Having established (P)RR expression in the heart, we next investigated (P)RR expression in other organs. At the mRNA level, maximal (P)RR expression was observed in brain, followed by kidney, intestine, liver and heart (Fig. 3A). In muscle and lung, expression was nearly absent. At the protein level, maximal expression was observed in kidney followed by liver, brain, heart and intestine (Fig. 3B and C). Again, expression was low in muscle and lung. Except for brain, mRNA and protein expression levels paralleled each other.

Bottom Line: Because none of the tested commercially available antibodies appeared to detect endogenous (P)RR, a (P)RR-specific polyclonal antibody was generated to study (P)RR protein levels. (P)RR protein levels were significantly increased in the post-MI rat heart (1.4-fold, P < 0.05) as compared to controls.Most interestingly in DCM patients, a significant 8.7-fold (P < 0.05) increase was observed.Thus, protein expression paralleled gene expression.

View Article: PubMed Central - PubMed

Affiliation: University Medical Center Groningen, Thorax Center, Department of Cardiology, University of Groningen, The Netherlands.

Show MeSH
Related in: MedlinePlus