Limits...
A possible role of thymidine phosphorylase expression and 5-fluorouracil increased sensitivity in oropharyngeal cancer patients.

Ranieri G, Grammatica L, Patruno R, Zito AF, Valerio P, Iacobellis S, Gadaleta C, Gasparini G, Ribatti D - J. Cell. Mol. Med. (2007)

Bottom Line: In this study, we have analysed by immunohistochemistry CD34, CD68 and TP positive cells in bioptic samples from 53 patients with T(1-3) N(0-1) M(0) oropharyngeal squamous cell carcinoma (OSC) and from 24 patients with non-dysplastic oropharyngeal leukoplakia (NDOLP).Results showed that the mean of TP-positive cells, CD68 positive macrophages and CD34 positive endothelial cells eval-uated as microvessel density (MVD) was significantly higher in OSC than in NDOLP.Moreover, at a median follow-up of 19 months, patients with TP expression and higher MVD showed a better survival rate as compared to those with low MVD, probably as a consequence of 5-FU-based therapy.We hypothesized a role for TP in oropharyngeal tumourigenesis and 5-FU activation in the adjuvant setting of OSC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Oncology, Unit of Interventional Radiology, Department of Critical Area and Surgery; National Cancer Institute of Bari, Bari, Italy.

ABSTRACT
Thymidine Pi deoxyribosyltransferase (TP) is an enzyme involved in DNA synthesis up-regulated in tumours and it is also a pro-angiogenic factor. TP cannot activate capecitabine, because capecitabine first needs conversion by carboxylesterase and cytidine deaminase into 5-deoxy-fluorouridine. This compound can be activated by TP to 5-fluorouracil (5-FU). Although TP is not necessary for 5-FU toxicity, experimental data suggest that high levels of TP correlate with an enhanced response to 5-FU therapy. In this study, we have analysed by immunohistochemistry CD34, CD68 and TP positive cells in bioptic samples from 53 patients with T(1-3) N(0-1) M(0) oropharyngeal squamous cell carcinoma (OSC) and from 24 patients with non-dysplastic oropharyngeal leukoplakia (NDOLP). Results showed that the mean of TP-positive cells, CD68 positive macrophages and CD34 positive endothelial cells eval-uated as microvessel density (MVD) was significantly higher in OSC than in NDOLP. Moreover, at a median follow-up of 19 months, patients with TP expression and higher MVD showed a better survival rate as compared to those with low MVD, probably as a consequence of 5-FU-based therapy.We hypothesized a role for TP in oropharyngeal tumourigenesis and 5-FU activation in the adjuvant setting of OSC patients.

Show MeSH

Related in: MedlinePlus

MVD positive to TP and overall survival curves in 53 oropharyngeal squamous cell carcinoma. At a median follow-up of 19 months, patients with high MVD tumours showed a better survival rate as compared to those with low MVD (75% and 45%, respectively).
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3822834&req=5

fig04: MVD positive to TP and overall survival curves in 53 oropharyngeal squamous cell carcinoma. At a median follow-up of 19 months, patients with high MVD tumours showed a better survival rate as compared to those with low MVD (75% and 45%, respectively).

Mentions: As concerns the margins of the surgical specimens, due to tumour size and in particular tumour location at oropharyngeal sites, radical surgery was obtained in 14 cases, while 39 patients presented positive resection margins. At a median follow-up of 19 months, a significant better overall survival (OS) was found for the OSC patients with the higher MVD positive to TP as compared to the OSC patients with the lower MVD positive to TP (75% and 45%, respectively; P = 0.03 log-rank test) (Fig. 4).


A possible role of thymidine phosphorylase expression and 5-fluorouracil increased sensitivity in oropharyngeal cancer patients.

Ranieri G, Grammatica L, Patruno R, Zito AF, Valerio P, Iacobellis S, Gadaleta C, Gasparini G, Ribatti D - J. Cell. Mol. Med. (2007)

MVD positive to TP and overall survival curves in 53 oropharyngeal squamous cell carcinoma. At a median follow-up of 19 months, patients with high MVD tumours showed a better survival rate as compared to those with low MVD (75% and 45%, respectively).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822834&req=5

fig04: MVD positive to TP and overall survival curves in 53 oropharyngeal squamous cell carcinoma. At a median follow-up of 19 months, patients with high MVD tumours showed a better survival rate as compared to those with low MVD (75% and 45%, respectively).
Mentions: As concerns the margins of the surgical specimens, due to tumour size and in particular tumour location at oropharyngeal sites, radical surgery was obtained in 14 cases, while 39 patients presented positive resection margins. At a median follow-up of 19 months, a significant better overall survival (OS) was found for the OSC patients with the higher MVD positive to TP as compared to the OSC patients with the lower MVD positive to TP (75% and 45%, respectively; P = 0.03 log-rank test) (Fig. 4).

Bottom Line: In this study, we have analysed by immunohistochemistry CD34, CD68 and TP positive cells in bioptic samples from 53 patients with T(1-3) N(0-1) M(0) oropharyngeal squamous cell carcinoma (OSC) and from 24 patients with non-dysplastic oropharyngeal leukoplakia (NDOLP).Results showed that the mean of TP-positive cells, CD68 positive macrophages and CD34 positive endothelial cells eval-uated as microvessel density (MVD) was significantly higher in OSC than in NDOLP.Moreover, at a median follow-up of 19 months, patients with TP expression and higher MVD showed a better survival rate as compared to those with low MVD, probably as a consequence of 5-FU-based therapy.We hypothesized a role for TP in oropharyngeal tumourigenesis and 5-FU activation in the adjuvant setting of OSC patients.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Oncology, Unit of Interventional Radiology, Department of Critical Area and Surgery; National Cancer Institute of Bari, Bari, Italy.

ABSTRACT
Thymidine Pi deoxyribosyltransferase (TP) is an enzyme involved in DNA synthesis up-regulated in tumours and it is also a pro-angiogenic factor. TP cannot activate capecitabine, because capecitabine first needs conversion by carboxylesterase and cytidine deaminase into 5-deoxy-fluorouridine. This compound can be activated by TP to 5-fluorouracil (5-FU). Although TP is not necessary for 5-FU toxicity, experimental data suggest that high levels of TP correlate with an enhanced response to 5-FU therapy. In this study, we have analysed by immunohistochemistry CD34, CD68 and TP positive cells in bioptic samples from 53 patients with T(1-3) N(0-1) M(0) oropharyngeal squamous cell carcinoma (OSC) and from 24 patients with non-dysplastic oropharyngeal leukoplakia (NDOLP). Results showed that the mean of TP-positive cells, CD68 positive macrophages and CD34 positive endothelial cells eval-uated as microvessel density (MVD) was significantly higher in OSC than in NDOLP. Moreover, at a median follow-up of 19 months, patients with TP expression and higher MVD showed a better survival rate as compared to those with low MVD, probably as a consequence of 5-FU-based therapy.We hypothesized a role for TP in oropharyngeal tumourigenesis and 5-FU activation in the adjuvant setting of OSC patients.

Show MeSH
Related in: MedlinePlus