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Roles of CD147 on T lymphocytes activation and MMP-9 secretion in systemic lupus erythematosus.

Pistol G, Matache C, Calugaru A, Stavaru C, Tanaseanu S, Ionescu R, Dumitrache S, Stefanescu M - J. Cell. Mol. Med. (2007 Mar-Apr)

Bottom Line: The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear.Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients.However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.

View Article: PubMed Central - PubMed

Affiliation: Centre for Advanced Studies, Cellular Receptors Laboratory, Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Splaiul Independentei 103, Bucharest 050096, Romania.

ABSTRACT
The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear. Some of these abnormalities referred to the hyperactivation of T lymphocytes and the enhanced secretion of MMP-9 by peripheral blood mononuclear cells (PBMCs). Therefore, in this paper we investigated the potential role of CD147 molecule in these abnormalities. Our results demonstrated that CD147 molecule is overexpressed on CD3+T lymphocytes from SLE patients when compared with CD3+T lymphocytes from healthy donors. Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients. However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.

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Related in: MedlinePlus

Modulation of MMPs activity in PBMCs cultured in absence or presence of monoclonal anti-CD147 antibodies. PBMCs of one SLE patient were cultured in absence (Line 2) or presence of anti-CD147 antibodies immobilized by rabbit anti-mouse IgG antibodies (Lines 4, 5) or soluble (lines 6, 7). Two controls were included in all experiments: positive control (fetal calf serum as source of latent and active forms of MMP-9 and latent MMP-2) (Line 1) and negative control (supernatant of PBMCs cultured only in the presence of rabbit antimouse IgG antibodies) (Line 3). After 24 hours, supernatants were collected and analyzed by gelatin zymography.
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fig08: Modulation of MMPs activity in PBMCs cultured in absence or presence of monoclonal anti-CD147 antibodies. PBMCs of one SLE patient were cultured in absence (Line 2) or presence of anti-CD147 antibodies immobilized by rabbit anti-mouse IgG antibodies (Lines 4, 5) or soluble (lines 6, 7). Two controls were included in all experiments: positive control (fetal calf serum as source of latent and active forms of MMP-9 and latent MMP-2) (Line 1) and negative control (supernatant of PBMCs cultured only in the presence of rabbit antimouse IgG antibodies) (Line 3). After 24 hours, supernatants were collected and analyzed by gelatin zymography.

Mentions: In order to verify if the blocking of CD147 molecules could inhibit the secretion of MMPs, SLE PBMCs were cultured for 24 hrs in the absence or presence of soluble or immobilized monoclonal anti-CD147 antibodies. The obtained results demonstrated that this anti-CD147 antibodies clone did not inhibit the activity of different types of MMPs, as it can be seen in Figure 8.


Roles of CD147 on T lymphocytes activation and MMP-9 secretion in systemic lupus erythematosus.

Pistol G, Matache C, Calugaru A, Stavaru C, Tanaseanu S, Ionescu R, Dumitrache S, Stefanescu M - J. Cell. Mol. Med. (2007 Mar-Apr)

Modulation of MMPs activity in PBMCs cultured in absence or presence of monoclonal anti-CD147 antibodies. PBMCs of one SLE patient were cultured in absence (Line 2) or presence of anti-CD147 antibodies immobilized by rabbit anti-mouse IgG antibodies (Lines 4, 5) or soluble (lines 6, 7). Two controls were included in all experiments: positive control (fetal calf serum as source of latent and active forms of MMP-9 and latent MMP-2) (Line 1) and negative control (supernatant of PBMCs cultured only in the presence of rabbit antimouse IgG antibodies) (Line 3). After 24 hours, supernatants were collected and analyzed by gelatin zymography.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822832&req=5

fig08: Modulation of MMPs activity in PBMCs cultured in absence or presence of monoclonal anti-CD147 antibodies. PBMCs of one SLE patient were cultured in absence (Line 2) or presence of anti-CD147 antibodies immobilized by rabbit anti-mouse IgG antibodies (Lines 4, 5) or soluble (lines 6, 7). Two controls were included in all experiments: positive control (fetal calf serum as source of latent and active forms of MMP-9 and latent MMP-2) (Line 1) and negative control (supernatant of PBMCs cultured only in the presence of rabbit antimouse IgG antibodies) (Line 3). After 24 hours, supernatants were collected and analyzed by gelatin zymography.
Mentions: In order to verify if the blocking of CD147 molecules could inhibit the secretion of MMPs, SLE PBMCs were cultured for 24 hrs in the absence or presence of soluble or immobilized monoclonal anti-CD147 antibodies. The obtained results demonstrated that this anti-CD147 antibodies clone did not inhibit the activity of different types of MMPs, as it can be seen in Figure 8.

Bottom Line: The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear.Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients.However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.

View Article: PubMed Central - PubMed

Affiliation: Centre for Advanced Studies, Cellular Receptors Laboratory, Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Splaiul Independentei 103, Bucharest 050096, Romania.

ABSTRACT
The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear. Some of these abnormalities referred to the hyperactivation of T lymphocytes and the enhanced secretion of MMP-9 by peripheral blood mononuclear cells (PBMCs). Therefore, in this paper we investigated the potential role of CD147 molecule in these abnormalities. Our results demonstrated that CD147 molecule is overexpressed on CD3+T lymphocytes from SLE patients when compared with CD3+T lymphocytes from healthy donors. Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients. However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.

Show MeSH
Related in: MedlinePlus