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Roles of CD147 on T lymphocytes activation and MMP-9 secretion in systemic lupus erythematosus.

Pistol G, Matache C, Calugaru A, Stavaru C, Tanaseanu S, Ionescu R, Dumitrache S, Stefanescu M - J. Cell. Mol. Med. (2007 Mar-Apr)

Bottom Line: The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear.Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients.However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.

View Article: PubMed Central - PubMed

Affiliation: Centre for Advanced Studies, Cellular Receptors Laboratory, Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Splaiul Independentei 103, Bucharest 050096, Romania.

ABSTRACT
The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear. Some of these abnormalities referred to the hyperactivation of T lymphocytes and the enhanced secretion of MMP-9 by peripheral blood mononuclear cells (PBMCs). Therefore, in this paper we investigated the potential role of CD147 molecule in these abnormalities. Our results demonstrated that CD147 molecule is overexpressed on CD3+T lymphocytes from SLE patients when compared with CD3+T lymphocytes from healthy donors. Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients. However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.

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Correlation between the percentage of CD3+CD147+T lymphocytes and the activity of secreted MMP-9. PBMCs freshly isolated from peripheral blood of SLE patients were analyzed by FACS in order to determine the percentage of CD3+CD147+T lymphocytes. In addition, PBMCs from the same patients were cultured for 24 hrs and the culture supernatants were analyzed by gelatin zymography. Zymograms were scanned and the intensity of bands was determined. Subsequently, these two parameters were correlated by Spearman's analysis.
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fig07: Correlation between the percentage of CD3+CD147+T lymphocytes and the activity of secreted MMP-9. PBMCs freshly isolated from peripheral blood of SLE patients were analyzed by FACS in order to determine the percentage of CD3+CD147+T lymphocytes. In addition, PBMCs from the same patients were cultured for 24 hrs and the culture supernatants were analyzed by gelatin zymography. Zymograms were scanned and the intensity of bands was determined. Subsequently, these two parameters were correlated by Spearman's analysis.

Mentions: Literature data demonstrated the role of CD147 molecules in MMPs induction [22–24]. On the other hand, it is known that SLE PBMCs produce and secrete large amounts of MMP-9 as compared with healthy donor PBMCs [9]. To test if secreted MMP-9 activity is related to CD147 expression, we analyzed these two parameters for PBMCs isolated from ten SLE patients (Fig. 7). Spearman's analysis showed a significant direct correlation between the percentage of CD3+CD147+T lymphocytes and the activity of MMP-9 secreted in supernatants of cultured SLE PBMCs (r = 0.627, p = 0.038).


Roles of CD147 on T lymphocytes activation and MMP-9 secretion in systemic lupus erythematosus.

Pistol G, Matache C, Calugaru A, Stavaru C, Tanaseanu S, Ionescu R, Dumitrache S, Stefanescu M - J. Cell. Mol. Med. (2007 Mar-Apr)

Correlation between the percentage of CD3+CD147+T lymphocytes and the activity of secreted MMP-9. PBMCs freshly isolated from peripheral blood of SLE patients were analyzed by FACS in order to determine the percentage of CD3+CD147+T lymphocytes. In addition, PBMCs from the same patients were cultured for 24 hrs and the culture supernatants were analyzed by gelatin zymography. Zymograms were scanned and the intensity of bands was determined. Subsequently, these two parameters were correlated by Spearman's analysis.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822832&req=5

fig07: Correlation between the percentage of CD3+CD147+T lymphocytes and the activity of secreted MMP-9. PBMCs freshly isolated from peripheral blood of SLE patients were analyzed by FACS in order to determine the percentage of CD3+CD147+T lymphocytes. In addition, PBMCs from the same patients were cultured for 24 hrs and the culture supernatants were analyzed by gelatin zymography. Zymograms were scanned and the intensity of bands was determined. Subsequently, these two parameters were correlated by Spearman's analysis.
Mentions: Literature data demonstrated the role of CD147 molecules in MMPs induction [22–24]. On the other hand, it is known that SLE PBMCs produce and secrete large amounts of MMP-9 as compared with healthy donor PBMCs [9]. To test if secreted MMP-9 activity is related to CD147 expression, we analyzed these two parameters for PBMCs isolated from ten SLE patients (Fig. 7). Spearman's analysis showed a significant direct correlation between the percentage of CD3+CD147+T lymphocytes and the activity of MMP-9 secreted in supernatants of cultured SLE PBMCs (r = 0.627, p = 0.038).

Bottom Line: The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear.Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients.However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.

View Article: PubMed Central - PubMed

Affiliation: Centre for Advanced Studies, Cellular Receptors Laboratory, Cantacuzino National Institute of Research and Development for Microbiology and Immunology, Splaiul Independentei 103, Bucharest 050096, Romania.

ABSTRACT
The cellular and molecular mechanisms involved in many abnormalities described in Systemic Lupus Erythematosus (SLE) are still unclear. Some of these abnormalities referred to the hyperactivation of T lymphocytes and the enhanced secretion of MMP-9 by peripheral blood mononuclear cells (PBMCs). Therefore, in this paper we investigated the potential role of CD147 molecule in these abnormalities. Our results demonstrated that CD147 molecule is overexpressed on CD3+T lymphocytes from SLE patients when compared with CD3+T lymphocytes from healthy donors. Monoclonal anti-CD147 antibodies, MEM-M6/1 clone, were able to inhibit protein tyrosine phosphorylation only in CD3 x CD28 costimulated T lymphocytes from SLE patients. However, this monoclonal antibody was unable to inhibit the enhanced activity of MMP-9 secreted by SLE PBMCs.

Show MeSH
Related in: MedlinePlus