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HOXC6 and HOXC11 increase transcription of S100beta gene in BrdU-induced in vitro differentiation of GOTO neuroblastoma cells into Schwannian cells.

Zhang X, Hamada J, Nishimoto A, Takahashi Y, Murai T, Tada M, Moriuchi T - J. Cell. Mol. Med. (2007 Mar-Apr)

Bottom Line: Forced expression of HOXC11 alone or both HOXC6 isoform 1 and HOXC11 induced the expression of S100beta in GOTO cells.In transient transfection experiments, the overexpression of HOXC6 and HOXC11 transactivated the S100beta promoter-reporter construct.Taken together, our results suggest that HOXC6 and HOXC11 are associated with differentiation of GOTO cells into Schwannian cells through the transcriptional activation of S100beta gene.

View Article: PubMed Central - PubMed

Affiliation: Division of Cancer-Related Genes, Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo 060-0815, Japan. doggirl999@hotmail.com

ABSTRACT
HOX genes encode transcription factors that play a key role in morphogenesis and cell differentiation during embryogenesis of animals. Human neuroblastoma cells are known to be chemically induced to differentiate into neuronal or Schwannian cells. In the present study, we investigated the roles of HOX genes in differentiation of GOTO neuroblastoma cells into Schwannian cells. When GOTO cells were grown in the presence of 5-bromo-2'-deoxyuridine (BrdU), they increased the expressions of two HOX genes (HOXC6 and HOXC11) and marker genes for Schwannian cells (S100beta and myelin basic protein). Forced expression of HOXC11 alone or both HOXC6 isoform 1 and HOXC11 induced the expression of S100beta in GOTO cells. In transient transfection experiments, the overexpression of HOXC6 and HOXC11 transactivated the S100beta promoter-reporter construct. Taken together, our results suggest that HOXC6 and HOXC11 are associated with differentiation of GOTO cells into Schwannian cells through the transcriptional activation of S100beta gene.

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Related in: MedlinePlus

Expressions of S100β and myelin basic protein genes in GOTO cells grown in the presence of BrdU. Total RNA was extracted from the cells cultured in the presence of 5 μg/ml of BrdU (B), 1 mM dbcAMP (A) or no chemicals (C) for the indicated period (days). The total RNA was reverse-transcribed, and a duplex PCR with primers for S100β or MBP and for β-actin was performed. PCR products were electrophoresed and stained with ethidium bromide.
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fig01: Expressions of S100β and myelin basic protein genes in GOTO cells grown in the presence of BrdU. Total RNA was extracted from the cells cultured in the presence of 5 μg/ml of BrdU (B), 1 mM dbcAMP (A) or no chemicals (C) for the indicated period (days). The total RNA was reverse-transcribed, and a duplex PCR with primers for S100β or MBP and for β-actin was performed. PCR products were electrophoresed and stained with ethidium bromide.

Mentions: First, we confirmed that GOTO cells differentiated into Schwannian cell-like cells by the treatment with 5-bromo-2′-deoxyuridine (BrdU). GOTO cells were cultured in the presence of 5 μg/ml of BrdU for 1–9 days, and their expression levels of S100β and myelin basic protein (MBP) as markers of Schwannian cells were detected by RT-duplex PCR. As shown in Figure 1, when GOTO cells were treated with BrdU, S100β and MBP were detected after 3 day- and 5 day-incubation, respectively, and their expression levels were maintained till day 9. When GOTO cells were incubated in the presence of 1 mM dibutyryl cyclic AMP (dbcAMP) which was known to induce differention into neuronal cells, the expression level of neither S100β nor MBP was affected.


HOXC6 and HOXC11 increase transcription of S100beta gene in BrdU-induced in vitro differentiation of GOTO neuroblastoma cells into Schwannian cells.

Zhang X, Hamada J, Nishimoto A, Takahashi Y, Murai T, Tada M, Moriuchi T - J. Cell. Mol. Med. (2007 Mar-Apr)

Expressions of S100β and myelin basic protein genes in GOTO cells grown in the presence of BrdU. Total RNA was extracted from the cells cultured in the presence of 5 μg/ml of BrdU (B), 1 mM dbcAMP (A) or no chemicals (C) for the indicated period (days). The total RNA was reverse-transcribed, and a duplex PCR with primers for S100β or MBP and for β-actin was performed. PCR products were electrophoresed and stained with ethidium bromide.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822828&req=5

fig01: Expressions of S100β and myelin basic protein genes in GOTO cells grown in the presence of BrdU. Total RNA was extracted from the cells cultured in the presence of 5 μg/ml of BrdU (B), 1 mM dbcAMP (A) or no chemicals (C) for the indicated period (days). The total RNA was reverse-transcribed, and a duplex PCR with primers for S100β or MBP and for β-actin was performed. PCR products were electrophoresed and stained with ethidium bromide.
Mentions: First, we confirmed that GOTO cells differentiated into Schwannian cell-like cells by the treatment with 5-bromo-2′-deoxyuridine (BrdU). GOTO cells were cultured in the presence of 5 μg/ml of BrdU for 1–9 days, and their expression levels of S100β and myelin basic protein (MBP) as markers of Schwannian cells were detected by RT-duplex PCR. As shown in Figure 1, when GOTO cells were treated with BrdU, S100β and MBP were detected after 3 day- and 5 day-incubation, respectively, and their expression levels were maintained till day 9. When GOTO cells were incubated in the presence of 1 mM dibutyryl cyclic AMP (dbcAMP) which was known to induce differention into neuronal cells, the expression level of neither S100β nor MBP was affected.

Bottom Line: Forced expression of HOXC11 alone or both HOXC6 isoform 1 and HOXC11 induced the expression of S100beta in GOTO cells.In transient transfection experiments, the overexpression of HOXC6 and HOXC11 transactivated the S100beta promoter-reporter construct.Taken together, our results suggest that HOXC6 and HOXC11 are associated with differentiation of GOTO cells into Schwannian cells through the transcriptional activation of S100beta gene.

View Article: PubMed Central - PubMed

Affiliation: Division of Cancer-Related Genes, Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo 060-0815, Japan. doggirl999@hotmail.com

ABSTRACT
HOX genes encode transcription factors that play a key role in morphogenesis and cell differentiation during embryogenesis of animals. Human neuroblastoma cells are known to be chemically induced to differentiate into neuronal or Schwannian cells. In the present study, we investigated the roles of HOX genes in differentiation of GOTO neuroblastoma cells into Schwannian cells. When GOTO cells were grown in the presence of 5-bromo-2'-deoxyuridine (BrdU), they increased the expressions of two HOX genes (HOXC6 and HOXC11) and marker genes for Schwannian cells (S100beta and myelin basic protein). Forced expression of HOXC11 alone or both HOXC6 isoform 1 and HOXC11 induced the expression of S100beta in GOTO cells. In transient transfection experiments, the overexpression of HOXC6 and HOXC11 transactivated the S100beta promoter-reporter construct. Taken together, our results suggest that HOXC6 and HOXC11 are associated with differentiation of GOTO cells into Schwannian cells through the transcriptional activation of S100beta gene.

Show MeSH
Related in: MedlinePlus