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Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation.

Fariha MM, Chua KH, Tan GC, Lim YH, Hayati AR - J. Cell. Mol. Med. (2013)

Bottom Line: High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC.The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues.Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia.

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(A) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. The vascular-like networks formed by HUVECS, hCDSC and mix culture of HUVECS-hCDSC were stained by Haematoxylin & Eosin. (B) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. Immunostaining of PECAM-1, vWF and α-SMA were performed on vascular-like network formed by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) in the three dimensional fibrin-matrigel construct in vivo. The red scale bars represent the size of 30 μm. (C) H&E staining of empty FMC harvested from in vivo experiment (A-i, ×100 and A-ii, ×200). The arrow showed infiltration of mouse cells. Mouse vessels stained with PECAM-1 antibody, with (B-iv, ×100) and without (B-iii, ×100) Rodent block M blocking reaction.
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fig05: (A) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. The vascular-like networks formed by HUVECS, hCDSC and mix culture of HUVECS-hCDSC were stained by Haematoxylin & Eosin. (B) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. Immunostaining of PECAM-1, vWF and α-SMA were performed on vascular-like network formed by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) in the three dimensional fibrin-matrigel construct in vivo. The red scale bars represent the size of 30 μm. (C) H&E staining of empty FMC harvested from in vivo experiment (A-i, ×100 and A-ii, ×200). The arrow showed infiltration of mouse cells. Mouse vessels stained with PECAM-1 antibody, with (B-iv, ×100) and without (B-iii, ×100) Rodent block M blocking reaction.

Mentions: As shown by HUVECS, hCDSC constructs were also capable of forming vascular structures in vivo. The presence of red blood cells in the majority of the vessels indicates that the vessels are functioning (Fig. 5A). There was a relative increase each in the number and the size of vessels in the hCDSC and HUVECS co-culture as comparedwith hCDSC alone. Most of the vessels showed an endothelial lining with very thin or absence of the smooth muscle layer, the latter being structures of capillaries or small venules. Immunohistochemistry showed positive expressions of PECAM-1, vWF and α-SMA in all types of constructs except for HUVECS which were positive for PECAM-1 and vWF only (Fig. 5B). Figure 5C-panel B showed that the Rodent Block M reactions had successfully prevented cross reaction of the antibody being used with the host's cells. Infiltration of the host's cells into the periphery of the empty FMC was observed on H&E staining (Fig. 5C-A).


Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation.

Fariha MM, Chua KH, Tan GC, Lim YH, Hayati AR - J. Cell. Mol. Med. (2013)

(A) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. The vascular-like networks formed by HUVECS, hCDSC and mix culture of HUVECS-hCDSC were stained by Haematoxylin & Eosin. (B) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. Immunostaining of PECAM-1, vWF and α-SMA were performed on vascular-like network formed by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) in the three dimensional fibrin-matrigel construct in vivo. The red scale bars represent the size of 30 μm. (C) H&E staining of empty FMC harvested from in vivo experiment (A-i, ×100 and A-ii, ×200). The arrow showed infiltration of mouse cells. Mouse vessels stained with PECAM-1 antibody, with (B-iv, ×100) and without (B-iii, ×100) Rodent block M blocking reaction.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3822821&req=5

fig05: (A) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. The vascular-like networks formed by HUVECS, hCDSC and mix culture of HUVECS-hCDSC were stained by Haematoxylin & Eosin. (B) Three dimensional fibrin-matrigel construct with different groups of cells harvested from in vivo experiment. Immunostaining of PECAM-1, vWF and α-SMA were performed on vascular-like network formed by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) in the three dimensional fibrin-matrigel construct in vivo. The red scale bars represent the size of 30 μm. (C) H&E staining of empty FMC harvested from in vivo experiment (A-i, ×100 and A-ii, ×200). The arrow showed infiltration of mouse cells. Mouse vessels stained with PECAM-1 antibody, with (B-iv, ×100) and without (B-iii, ×100) Rodent block M blocking reaction.
Mentions: As shown by HUVECS, hCDSC constructs were also capable of forming vascular structures in vivo. The presence of red blood cells in the majority of the vessels indicates that the vessels are functioning (Fig. 5A). There was a relative increase each in the number and the size of vessels in the hCDSC and HUVECS co-culture as comparedwith hCDSC alone. Most of the vessels showed an endothelial lining with very thin or absence of the smooth muscle layer, the latter being structures of capillaries or small venules. Immunohistochemistry showed positive expressions of PECAM-1, vWF and α-SMA in all types of constructs except for HUVECS which were positive for PECAM-1 and vWF only (Fig. 5B). Figure 5C-panel B showed that the Rodent Block M reactions had successfully prevented cross reaction of the antibody being used with the host's cells. Infiltration of the host's cells into the periphery of the empty FMC was observed on H&E staining (Fig. 5C-A).

Bottom Line: High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC.The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues.Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia.

Show MeSH
Related in: MedlinePlus