Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation.
Bottom Line: High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC.The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues.Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.
Affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia.Show MeSH
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Mentions: As shown by HUVECS, hCDSC constructs were also capable of forming vascular structures in vivo. The presence of red blood cells in the majority of the vessels indicates that the vessels are functioning (Fig. 5A). There was a relative increase each in the number and the size of vessels in the hCDSC and HUVECS co-culture as comparedwith hCDSC alone. Most of the vessels showed an endothelial lining with very thin or absence of the smooth muscle layer, the latter being structures of capillaries or small venules. Immunohistochemistry showed positive expressions of PECAM-1, vWF and α-SMA in all types of constructs except for HUVECS which were positive for PECAM-1 and vWF only (Fig. 5B). Figure 5C-panel B showed that the Rodent Block M reactions had successfully prevented cross reaction of the antibody being used with the host's cells. Infiltration of the host's cells into the periphery of the empty FMC was observed on H&E staining (Fig. 5C-A).
Affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia.