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Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation.

Fariha MM, Chua KH, Tan GC, Lim YH, Hayati AR - J. Cell. Mol. Med. (2013)

Bottom Line: High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC.The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues.Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia.

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Live imaging of hCDSC (green) and HUVECS (red) co-culture in fibrin-matrigel construct after 3, 9 and 15 days. The overlapping of hCDSC and HUVECS were marked by yellow colour.
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fig04: Live imaging of hCDSC (green) and HUVECS (red) co-culture in fibrin-matrigel construct after 3, 9 and 15 days. The overlapping of hCDSC and HUVECS were marked by yellow colour.

Mentions: When we mixed hCDSC with HUVECS in 3D constructs and tracked the cells through live imaging confocal microscopy, it was shown that both types of cells were present in the vascular-like structures (Fig. 4). Cell clusters comprising of both hCDSC and HUVECS were noticeable on day 3. On day 9 and 15, the vascular network increased in density and length, the size of the vessels formed increased by 2 μm and the cooperation of both types of cells were more obvious.


Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation.

Fariha MM, Chua KH, Tan GC, Lim YH, Hayati AR - J. Cell. Mol. Med. (2013)

Live imaging of hCDSC (green) and HUVECS (red) co-culture in fibrin-matrigel construct after 3, 9 and 15 days. The overlapping of hCDSC and HUVECS were marked by yellow colour.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3822821&req=5

fig04: Live imaging of hCDSC (green) and HUVECS (red) co-culture in fibrin-matrigel construct after 3, 9 and 15 days. The overlapping of hCDSC and HUVECS were marked by yellow colour.
Mentions: When we mixed hCDSC with HUVECS in 3D constructs and tracked the cells through live imaging confocal microscopy, it was shown that both types of cells were present in the vascular-like structures (Fig. 4). Cell clusters comprising of both hCDSC and HUVECS were noticeable on day 3. On day 9 and 15, the vascular network increased in density and length, the size of the vessels formed increased by 2 μm and the cooperation of both types of cells were more obvious.

Bottom Line: High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC.The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues.Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia.

Show MeSH