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Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation.

Fariha MM, Chua KH, Tan GC, Lim YH, Hayati AR - J. Cell. Mol. Med. (2013)

Bottom Line: High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC.The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues.Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia.

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(A) VEGF secretion by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) cultured in three dimensional fibrin-matrigel construct (in vitro) after 3, 6, 9 and 15 days of incubation. Culture medium for empty FMC culture was used as control. *Indicate significant difference when comparing hCDSC with control and HUVECS group while **indicate significant difference when comparing mix cells with control and HUVECS group (P < 0.05 and n = 6). (B) bFGF secretion by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) cultured in three dimensional fibrin-matrigel construct (in vitro) after 3, 6, 9 and 15 days of incubation. Culture medium for empty FMC culture was used as control. *Indicate significant difference when comparing hCDSC with control and HUVECS group while **indicate significant difference when comparing mix cells with control and HUVECS group (P < 0.05 and n = 6).
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fig02: (A) VEGF secretion by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) cultured in three dimensional fibrin-matrigel construct (in vitro) after 3, 6, 9 and 15 days of incubation. Culture medium for empty FMC culture was used as control. *Indicate significant difference when comparing hCDSC with control and HUVECS group while **indicate significant difference when comparing mix cells with control and HUVECS group (P < 0.05 and n = 6). (B) bFGF secretion by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) cultured in three dimensional fibrin-matrigel construct (in vitro) after 3, 6, 9 and 15 days of incubation. Culture medium for empty FMC culture was used as control. *Indicate significant difference when comparing hCDSC with control and HUVECS group while **indicate significant difference when comparing mix cells with control and HUVECS group (P < 0.05 and n = 6).

Mentions: Human CDSC demonstrated significantly (P < 0.05) higher VEGF (Fig. 2A) and bFGF (Fig. 2B) secretions when compared with the control and the HUVECS groups. Similar findings were observed when comparing mix cells with control and HUVECS group. However, the differences in both VEGF and bFGF secretions between hCDSC and mix cells (HUVECS + hCDSC) were not significant.


Pro-angiogenic potential of human chorion-derived stem cells: in vitro and in vivo evaluation.

Fariha MM, Chua KH, Tan GC, Lim YH, Hayati AR - J. Cell. Mol. Med. (2013)

(A) VEGF secretion by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) cultured in three dimensional fibrin-matrigel construct (in vitro) after 3, 6, 9 and 15 days of incubation. Culture medium for empty FMC culture was used as control. *Indicate significant difference when comparing hCDSC with control and HUVECS group while **indicate significant difference when comparing mix cells with control and HUVECS group (P < 0.05 and n = 6). (B) bFGF secretion by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) cultured in three dimensional fibrin-matrigel construct (in vitro) after 3, 6, 9 and 15 days of incubation. Culture medium for empty FMC culture was used as control. *Indicate significant difference when comparing hCDSC with control and HUVECS group while **indicate significant difference when comparing mix cells with control and HUVECS group (P < 0.05 and n = 6).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3822821&req=5

fig02: (A) VEGF secretion by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) cultured in three dimensional fibrin-matrigel construct (in vitro) after 3, 6, 9 and 15 days of incubation. Culture medium for empty FMC culture was used as control. *Indicate significant difference when comparing hCDSC with control and HUVECS group while **indicate significant difference when comparing mix cells with control and HUVECS group (P < 0.05 and n = 6). (B) bFGF secretion by HUVECS, hCDSC and mix cells (HUVECS and hCDSC) cultured in three dimensional fibrin-matrigel construct (in vitro) after 3, 6, 9 and 15 days of incubation. Culture medium for empty FMC culture was used as control. *Indicate significant difference when comparing hCDSC with control and HUVECS group while **indicate significant difference when comparing mix cells with control and HUVECS group (P < 0.05 and n = 6).
Mentions: Human CDSC demonstrated significantly (P < 0.05) higher VEGF (Fig. 2A) and bFGF (Fig. 2B) secretions when compared with the control and the HUVECS groups. Similar findings were observed when comparing mix cells with control and HUVECS group. However, the differences in both VEGF and bFGF secretions between hCDSC and mix cells (HUVECS + hCDSC) were not significant.

Bottom Line: High secretions of VEGF and bFGF by hCDSC with increased expressions of angiogenic and endogenic genes suggested the possible angiogenic promoting mechanisms by hCDSC.The cooperation of hCDSC with HUVECS to generate vessel-like structures in our systems is an indication that there will be positive interactions of hCDSC with existing endothelial cells when injected into ischaemic tissues.Hence, hCDSC is suggested as the novel approach in the future treatment of ischaemic diseases.

View Article: PubMed Central - PubMed

Affiliation: Department of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Cheras, Kuala Lumpur, Malaysia; Faculty of Medicine and Health Sciences, Universiti Sains Islam Malaysia, Kuala Lumpur, Malaysia.

Show MeSH