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Luteolin suppresses UVB-induced photoageing by targeting JNK1 and p90 RSK2.

Lim SH, Jung SK, Byun S, Lee EJ, Hwang JA, Seo SG, Kim YA, Yu JG, Lee KW, Lee HJ - J. Cell. Mol. Med. (2013)

Bottom Line: Luteolin, a bioactive compound found in chilli, onion, broccoli, celery and carrot, has been reported to exhibit anti-photoageing effects in vitro.Luteolin was found to inhibit UVB-induced MMP-1 expression in HaCaT cells, as well as UVB-induced activation of AP-1, a well-known transcription factor targeting the MMP-1 promoter region, as well as c-Fos and c-Jun, which comprise the AP-1 complex.Taken together, our observations suggest that luteolin exhibits anti-photoageing effects in vitro and in vivo and may have potential as a treatment for the prevention of skin ageing.

View Article: PubMed Central - PubMed

Affiliation: WCU Biomodulation Major, Center for Food and Bioconvergence, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.

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Effect of luteolin on UVB-induced AP-1 transactivation and c-Fos promoter activation in HaCaT human keratinocytes stably transfected with AP-1 and c-Fos luciferase reporter plasmids. (A and B) Luteolin inhibits both UVB-induced AP-1 transactivation and c-Fos promoter activation. For the luciferase assay, HaCaT cells stably transfected with AP-1 or c-Fos luciferase reporter plasmid were starved for 24 hrs in serum-free DMEM and pretreated with luteolin at the indicated concentrations (0, 5, 10 μM). After 1 hr, the cells were washed with PBS and then irradiated with UVB (0.01 J/cm2) in a small volume of PBS. The cells were then incubated for 5 hrs (A) or 3 hrs (B), and relative luciferase reporter activity was determined by analysing the cell lysates as described in Materials and Methods. AP-1 and c-Fos luciferase activities are expressed as the percent inhibition relative to non-UVB irradiated cells. Data are represented as the mean ±SD of AP-1 and c-Fos luciferase activity from three independent experiments (* and **significant differences at P < 0.05 and P < 0.01 between groups treated with UVB and luteolin and the group treated with UVB alone respectively).
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fig02: Effect of luteolin on UVB-induced AP-1 transactivation and c-Fos promoter activation in HaCaT human keratinocytes stably transfected with AP-1 and c-Fos luciferase reporter plasmids. (A and B) Luteolin inhibits both UVB-induced AP-1 transactivation and c-Fos promoter activation. For the luciferase assay, HaCaT cells stably transfected with AP-1 or c-Fos luciferase reporter plasmid were starved for 24 hrs in serum-free DMEM and pretreated with luteolin at the indicated concentrations (0, 5, 10 μM). After 1 hr, the cells were washed with PBS and then irradiated with UVB (0.01 J/cm2) in a small volume of PBS. The cells were then incubated for 5 hrs (A) or 3 hrs (B), and relative luciferase reporter activity was determined by analysing the cell lysates as described in Materials and Methods. AP-1 and c-Fos luciferase activities are expressed as the percent inhibition relative to non-UVB irradiated cells. Data are represented as the mean ±SD of AP-1 and c-Fos luciferase activity from three independent experiments (* and **significant differences at P < 0.05 and P < 0.01 between groups treated with UVB and luteolin and the group treated with UVB alone respectively).

Mentions: UVB is known to induce Activator Protein-1 (AP-1) and MMP-1 is a target gene with an AP-1 binding site in its promoter region 33, 34. The pretreatment of cells with luteolin inhibited UVB-induced AP-1 transactivation in HaCaT cells stably transfected with an AP-1 luciferase plasmid (Fig. 2A). We next investigated the effect of luteolin on UVB-induced c-Fos promoter activation. Pretreatment of cells with luteolin reduced UVB-induced c-Fos gene expression in HaCaT cells that were stably transfected with c-Fos luciferase plasmid (Fig. 2B).


Luteolin suppresses UVB-induced photoageing by targeting JNK1 and p90 RSK2.

Lim SH, Jung SK, Byun S, Lee EJ, Hwang JA, Seo SG, Kim YA, Yu JG, Lee KW, Lee HJ - J. Cell. Mol. Med. (2013)

Effect of luteolin on UVB-induced AP-1 transactivation and c-Fos promoter activation in HaCaT human keratinocytes stably transfected with AP-1 and c-Fos luciferase reporter plasmids. (A and B) Luteolin inhibits both UVB-induced AP-1 transactivation and c-Fos promoter activation. For the luciferase assay, HaCaT cells stably transfected with AP-1 or c-Fos luciferase reporter plasmid were starved for 24 hrs in serum-free DMEM and pretreated with luteolin at the indicated concentrations (0, 5, 10 μM). After 1 hr, the cells were washed with PBS and then irradiated with UVB (0.01 J/cm2) in a small volume of PBS. The cells were then incubated for 5 hrs (A) or 3 hrs (B), and relative luciferase reporter activity was determined by analysing the cell lysates as described in Materials and Methods. AP-1 and c-Fos luciferase activities are expressed as the percent inhibition relative to non-UVB irradiated cells. Data are represented as the mean ±SD of AP-1 and c-Fos luciferase activity from three independent experiments (* and **significant differences at P < 0.05 and P < 0.01 between groups treated with UVB and luteolin and the group treated with UVB alone respectively).
© Copyright Policy - open-access
Related In: Results  -  Collection

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fig02: Effect of luteolin on UVB-induced AP-1 transactivation and c-Fos promoter activation in HaCaT human keratinocytes stably transfected with AP-1 and c-Fos luciferase reporter plasmids. (A and B) Luteolin inhibits both UVB-induced AP-1 transactivation and c-Fos promoter activation. For the luciferase assay, HaCaT cells stably transfected with AP-1 or c-Fos luciferase reporter plasmid were starved for 24 hrs in serum-free DMEM and pretreated with luteolin at the indicated concentrations (0, 5, 10 μM). After 1 hr, the cells were washed with PBS and then irradiated with UVB (0.01 J/cm2) in a small volume of PBS. The cells were then incubated for 5 hrs (A) or 3 hrs (B), and relative luciferase reporter activity was determined by analysing the cell lysates as described in Materials and Methods. AP-1 and c-Fos luciferase activities are expressed as the percent inhibition relative to non-UVB irradiated cells. Data are represented as the mean ±SD of AP-1 and c-Fos luciferase activity from three independent experiments (* and **significant differences at P < 0.05 and P < 0.01 between groups treated with UVB and luteolin and the group treated with UVB alone respectively).
Mentions: UVB is known to induce Activator Protein-1 (AP-1) and MMP-1 is a target gene with an AP-1 binding site in its promoter region 33, 34. The pretreatment of cells with luteolin inhibited UVB-induced AP-1 transactivation in HaCaT cells stably transfected with an AP-1 luciferase plasmid (Fig. 2A). We next investigated the effect of luteolin on UVB-induced c-Fos promoter activation. Pretreatment of cells with luteolin reduced UVB-induced c-Fos gene expression in HaCaT cells that were stably transfected with c-Fos luciferase plasmid (Fig. 2B).

Bottom Line: Luteolin, a bioactive compound found in chilli, onion, broccoli, celery and carrot, has been reported to exhibit anti-photoageing effects in vitro.Luteolin was found to inhibit UVB-induced MMP-1 expression in HaCaT cells, as well as UVB-induced activation of AP-1, a well-known transcription factor targeting the MMP-1 promoter region, as well as c-Fos and c-Jun, which comprise the AP-1 complex.Taken together, our observations suggest that luteolin exhibits anti-photoageing effects in vitro and in vivo and may have potential as a treatment for the prevention of skin ageing.

View Article: PubMed Central - PubMed

Affiliation: WCU Biomodulation Major, Center for Food and Bioconvergence, Department of Agricultural Biotechnology, Seoul National University, Seoul, Republic of Korea.

Show MeSH
Related in: MedlinePlus