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Overexpression of Sema3a in myocardial infarction border zone decreases vulnerability of ventricular tachycardia post-myocardial infarction in rats.

Chen RH, Li YG, Jiao KL, Zhang PP, Sun Y, Zhang LP, Fong XF, Li W, Yu Y - J. Cell. Mol. Med. (2013)

Bottom Line: Inducibility of VT by PES was significantly lower in the SLV group [30.8% (4/13)] than in PBS [66.7% (8/12)] and MLV [61.5% (8/13)] groups (P < 0.05). mRNA and protein expressions of Sema3a were significantly higher and the protein expression of GAP43 and TH was significantly lower at 7 days after transduction in SLV group compared with PBS, MLV and CON groups.Myocardial EPI in the border zone was also significantly lower in SLV group than in PBS and MLV group (8.73 ± 1.30 vs. 11.94 ± 1.71 and 12.24 ± 1.54 μg/g protein, P < 0.001).Overexpression of Sema3a in MI border zone could reduce the inducibility of ventricular arrhythmias by reducing sympathetic hyper-reinnervation after infarction.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, School of Medicine, Xinhua Hospital, Shanghai Jiaotong University, Shanghai, China.

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Effect of SLV on Sema3a mRNA and protein expressions. The SLV could significantly up-regulate the Sema3a mRNA and protein level. A representative Western blot (A). Relative densitometric values of Western blots (B). Values were calculated from three independent experiments; *P < 0.05 versus the PBS group, †P < 0.05 versus the MLV group. (C) mRNA expression of Sema3a by RT-PCR in different groups. The values had been normalized to GAPDH measurement and then expressed as a ratio of normalized values to mRNA in static control group; *P < 0.05 versus the PBS group, †P < 0.05 versus the MLV group.
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fig05: Effect of SLV on Sema3a mRNA and protein expressions. The SLV could significantly up-regulate the Sema3a mRNA and protein level. A representative Western blot (A). Relative densitometric values of Western blots (B). Values were calculated from three independent experiments; *P < 0.05 versus the PBS group, †P < 0.05 versus the MLV group. (C) mRNA expression of Sema3a by RT-PCR in different groups. The values had been normalized to GAPDH measurement and then expressed as a ratio of normalized values to mRNA in static control group; *P < 0.05 versus the PBS group, †P < 0.05 versus the MLV group.

Mentions: Reporter gene expression examined by fluorescent microscopy demonstrated lentivirus-derived GFP+ labelled host cardiomyocytes at 1 week after either MLV or SLV injection, however, GFP+ cardiomyocytes cells were not observed in PBS group and in sham group (Fig. 4). The mRNA-Sema3a level in the myocardium of MI border zone in SLV groups was significantly higher (about twofold) at 1 week after transfection compared with PBS, MLV, CON groups (Fig. 5c).


Overexpression of Sema3a in myocardial infarction border zone decreases vulnerability of ventricular tachycardia post-myocardial infarction in rats.

Chen RH, Li YG, Jiao KL, Zhang PP, Sun Y, Zhang LP, Fong XF, Li W, Yu Y - J. Cell. Mol. Med. (2013)

Effect of SLV on Sema3a mRNA and protein expressions. The SLV could significantly up-regulate the Sema3a mRNA and protein level. A representative Western blot (A). Relative densitometric values of Western blots (B). Values were calculated from three independent experiments; *P < 0.05 versus the PBS group, †P < 0.05 versus the MLV group. (C) mRNA expression of Sema3a by RT-PCR in different groups. The values had been normalized to GAPDH measurement and then expressed as a ratio of normalized values to mRNA in static control group; *P < 0.05 versus the PBS group, †P < 0.05 versus the MLV group.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3822813&req=5

fig05: Effect of SLV on Sema3a mRNA and protein expressions. The SLV could significantly up-regulate the Sema3a mRNA and protein level. A representative Western blot (A). Relative densitometric values of Western blots (B). Values were calculated from three independent experiments; *P < 0.05 versus the PBS group, †P < 0.05 versus the MLV group. (C) mRNA expression of Sema3a by RT-PCR in different groups. The values had been normalized to GAPDH measurement and then expressed as a ratio of normalized values to mRNA in static control group; *P < 0.05 versus the PBS group, †P < 0.05 versus the MLV group.
Mentions: Reporter gene expression examined by fluorescent microscopy demonstrated lentivirus-derived GFP+ labelled host cardiomyocytes at 1 week after either MLV or SLV injection, however, GFP+ cardiomyocytes cells were not observed in PBS group and in sham group (Fig. 4). The mRNA-Sema3a level in the myocardium of MI border zone in SLV groups was significantly higher (about twofold) at 1 week after transfection compared with PBS, MLV, CON groups (Fig. 5c).

Bottom Line: Inducibility of VT by PES was significantly lower in the SLV group [30.8% (4/13)] than in PBS [66.7% (8/12)] and MLV [61.5% (8/13)] groups (P < 0.05). mRNA and protein expressions of Sema3a were significantly higher and the protein expression of GAP43 and TH was significantly lower at 7 days after transduction in SLV group compared with PBS, MLV and CON groups.Myocardial EPI in the border zone was also significantly lower in SLV group than in PBS and MLV group (8.73 ± 1.30 vs. 11.94 ± 1.71 and 12.24 ± 1.54 μg/g protein, P < 0.001).Overexpression of Sema3a in MI border zone could reduce the inducibility of ventricular arrhythmias by reducing sympathetic hyper-reinnervation after infarction.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiology, School of Medicine, Xinhua Hospital, Shanghai Jiaotong University, Shanghai, China.

Show MeSH
Related in: MedlinePlus