Glomerular parietal epithelial cells of adult murine kidney undergo EMT to generate cells with traits of renal progenitors.
Bottom Line: When injected into E13.5 kidney rudiments, the cells incorporated into the developing kidney primordia and co-culture with E13.5 spinal cord resulted in branching and tubulogenesis in these cells.When implanted under renal capsule of unilaterally nephrectomized mice, these cells differentiated into immature glomeruli and vascular ducts.Our study demonstrates that EMT plays a major role in imparting plasticity to terminally differentiated GPECs by producing metastable cells with traits of kidney progenitors.
Affiliation: Tissue Engineering and Banking Laboratory, National Centre for Cell Science, Pune, India.Show MeSH
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Mentions: Here, we demonstrate the ability of CD24+ cells to subsist, proliferate and integrate into the developing E13.5 kidney in an in vitro system. The CD24+ cells (1000–1500 cells/tissue) tagged with green fluorescent dye marker CMFDA were injected into the kidney rudiments dissected from E13.5 kidney and cultured for 5 days on transwell filters without additional growth factors. Whole mount and antibody staining of the rudiments showed that the CD24+ cells have integrated into the developing kidney. Optical sectioning of the whole mount kidney rudiment showed clusters of CD24+ cells integrated with the 3D kidney primordia (Fig. 6A). Anti-laminin staining showed that the cells have integrated into the developing tubules and uteric-bud stalk  (Fig. 6B). PGE2 treated cells failed to integrate into the developing kidney. However, few cells remained attached to kidney surface (Fig. 6B).
Affiliation: Tissue Engineering and Banking Laboratory, National Centre for Cell Science, Pune, India.