Ferroportin 1 is expressed basolaterally in rat kidney proximal tubule cells and iron excess increases its membrane trafficking.
Bottom Line: FPN1 was found to be located in association with the basolateral PT membrane and within the cytosolic compartment.These data support a role for FPN1 in vectorial export of iron out of PT cells.Furthermore, under conditions of iron loading of cultured PT cells, FPN1 was trafficked to the plasma membrane suggesting a coordinated cellular response to export excess iron and limit cellular iron concentrations.
Affiliation: Department of Physiology & Pathophysiology, University of Witten/Herdecke, Witten, Germany.Show MeSH
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Mentions: To visualize the exact cellular location of FPN1 in kidney PT, we immunostained kidneys using Liu-FPN1. Immunoperoxidase staining showed that Liu-FPN1 strongly stained PTs in the kidney cortex. Staining was absent from glomeruli and distal tubules (Fig. 2A). At higher magnification, FPN1 immunoreactivity was observed on the basal membrane and what appeared to be the inside of PT cells (Fig. 2B and C). This pattern of staining was evident in the S1, S2 and S3 segments of the PT (Fig. 2D and E). Staining appeared to be most prominent in the S2 segments, weaker in S1 segments and weakest in S3 segments.
Affiliation: Department of Physiology & Pathophysiology, University of Witten/Herdecke, Witten, Germany.