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Cell survival and redistribution after transplantation into damaged myocardium.

Zhang H, Chen H, Wang W, Wei Y, Hu S - J. Cell. Mol. Med. (2010)

Bottom Line: However, poor cell survival and extensive redistribution throughout the body can drastically affect the outcome and safety of cell therapy.Although various approaches have been attempted to support the survival and engraftment of implanted cells, we need to apply a new comprehensive strategy by melding the in vitro and in vivo approaches to recondition the cells and infarcted myocardium.Here we summarize our understanding of cell survival and migration after transplantation into the damaged heart.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiac Surgery, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing, China.

ABSTRACT
A hybrid approach to support cell survival and decrease cell escape Cell transplantation has become an attractive option for cardiac regenerative therapy. However, poor cell survival and extensive redistribution throughout the body can drastically affect the outcome and safety of cell therapy. Although various approaches have been attempted to support the survival and engraftment of implanted cells, we need to apply a new comprehensive strategy by melding the in vitro and in vivo approaches to recondition the cells and infarcted myocardium. Here we summarize our understanding of cell survival and migration after transplantation into the damaged heart.

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Illustration of the current preconditioning approaches to enhance cell survival.
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fig03: Illustration of the current preconditioning approaches to enhance cell survival.

Mentions: Various approaches have attempted to support the survival and engraftment of implanted cells (summarized in Fig. 3). In vitro gene modification could be very labour intensive and carry inherent risks for future clinical translation. Therefore, some more convenient approaches such as hypoxia, heat shock and low-level laser irradiation (LLLI) have also been applied for in vitro cell manipulation [16]. These approaches could induce faster cell proliferation, augment cytokine release and increase preconditioning or anti-hypoxia ability. Another easy way to enhance cell retention is to increase the size of the injected cells with microencapsulation [17]. The size of freshly isolated progenitor cells is usually 10–20 μm, so the cells could be packed into microcapsules (150–250 μm). With the increased size, the cells would not be drained to the myocardial vein, collateral channels or ventricular lumen and thus reside in the injection sites. Fabrication of cell-seeded biodegradable scaffolds is a promising tissue-engineering approach to nourish grafted cells and maintain neo-cardiac tissue geometry and structure [18]. However, many issues remain unsolved, including the optimal scaffold biomaterial, cell adhesion and diffusion of nutrients. Besides direct pre-treatment with cytokines, recently some small molecules have been identified as novel regulators that are capable of enhancing cell survival rates. Lysophosphatidic acid, as an endogenous lipid mediator, could prevent cell apoptotic death and increase cell survival 2-fold within 1 week after cell injection [19]. Recently, it was demonstrated that microRNAs participate in the ischemic preconditioning. The increased expression of miR-210 promoted transplanted cell survival in an acute MI model [20]. Tannic acid is a natural plant polyphenol. Local injection of tannic acid could cross-link fibrous collagen and inhibit matrix metalloproteinase activity [21]. The consequent stabilization of the extracellular matrix would reduce myocardial mechanical tension and thereby promote cell survival. These small molecules could be injected simultaneously into the damaged myocardium during CTx.


Cell survival and redistribution after transplantation into damaged myocardium.

Zhang H, Chen H, Wang W, Wei Y, Hu S - J. Cell. Mol. Med. (2010)

Illustration of the current preconditioning approaches to enhance cell survival.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822744&req=5

fig03: Illustration of the current preconditioning approaches to enhance cell survival.
Mentions: Various approaches have attempted to support the survival and engraftment of implanted cells (summarized in Fig. 3). In vitro gene modification could be very labour intensive and carry inherent risks for future clinical translation. Therefore, some more convenient approaches such as hypoxia, heat shock and low-level laser irradiation (LLLI) have also been applied for in vitro cell manipulation [16]. These approaches could induce faster cell proliferation, augment cytokine release and increase preconditioning or anti-hypoxia ability. Another easy way to enhance cell retention is to increase the size of the injected cells with microencapsulation [17]. The size of freshly isolated progenitor cells is usually 10–20 μm, so the cells could be packed into microcapsules (150–250 μm). With the increased size, the cells would not be drained to the myocardial vein, collateral channels or ventricular lumen and thus reside in the injection sites. Fabrication of cell-seeded biodegradable scaffolds is a promising tissue-engineering approach to nourish grafted cells and maintain neo-cardiac tissue geometry and structure [18]. However, many issues remain unsolved, including the optimal scaffold biomaterial, cell adhesion and diffusion of nutrients. Besides direct pre-treatment with cytokines, recently some small molecules have been identified as novel regulators that are capable of enhancing cell survival rates. Lysophosphatidic acid, as an endogenous lipid mediator, could prevent cell apoptotic death and increase cell survival 2-fold within 1 week after cell injection [19]. Recently, it was demonstrated that microRNAs participate in the ischemic preconditioning. The increased expression of miR-210 promoted transplanted cell survival in an acute MI model [20]. Tannic acid is a natural plant polyphenol. Local injection of tannic acid could cross-link fibrous collagen and inhibit matrix metalloproteinase activity [21]. The consequent stabilization of the extracellular matrix would reduce myocardial mechanical tension and thereby promote cell survival. These small molecules could be injected simultaneously into the damaged myocardium during CTx.

Bottom Line: However, poor cell survival and extensive redistribution throughout the body can drastically affect the outcome and safety of cell therapy.Although various approaches have been attempted to support the survival and engraftment of implanted cells, we need to apply a new comprehensive strategy by melding the in vitro and in vivo approaches to recondition the cells and infarcted myocardium.Here we summarize our understanding of cell survival and migration after transplantation into the damaged heart.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiac Surgery, Cardiovascular Institute and Fu Wai Hospital, Chinese Academy of Medical Sciences, Beijing, China.

ABSTRACT
A hybrid approach to support cell survival and decrease cell escape Cell transplantation has become an attractive option for cardiac regenerative therapy. However, poor cell survival and extensive redistribution throughout the body can drastically affect the outcome and safety of cell therapy. Although various approaches have been attempted to support the survival and engraftment of implanted cells, we need to apply a new comprehensive strategy by melding the in vitro and in vivo approaches to recondition the cells and infarcted myocardium. Here we summarize our understanding of cell survival and migration after transplantation into the damaged heart.

Show MeSH