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Engineered heart tissue graft derived from somatic cell nuclear transferred embryonic stem cells improve myocardial performance in infarcted rat heart.

Lü S, Li Y, Gao S, Liu S, Wang H, He W, Zhou J, Liu Z, Zhang Y, Lin Q, Duan C, Yang XJ, Wang C - J. Cell. Mol. Med. (2010)

Bottom Line: The EHTs, which were derived from NT-ES cells, inte grated and electrically coupled to host myocardium and exerted beneficial effects on the left ventricular function of infarcted rat heart.NT-ES cells can be used as a source of seeding cells for cardiac tissue engineering.Large contractile EHT grafts can be constructed in vitro with the ability to survive after implantation and improve myocardial performance of infarcted rat hearts.

View Article: PubMed Central - PubMed

Affiliation: Department of Tissue Engineering, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, China Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, China.

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Electrical coupling of EHT grafts to host myocardium. (A) Total activation time on the region of MI, right, left and posterior segments of the investigated hearts. (B) EHT could be uncoupled after acidification of the hearts. * P< 0.05 versus sham-operated MI rats by anova with Mann-Whitney U-test.
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fig05: Electrical coupling of EHT grafts to host myocardium. (A) Total activation time on the region of MI, right, left and posterior segments of the investigated hearts. (B) EHT could be uncoupled after acidification of the hearts. * P< 0.05 versus sham-operated MI rats by anova with Mann-Whitney U-test.

Mentions: We assessed electrical coupling of EHTs to the host myocardium 4 weeks after engraftment by MEA. The total activation time on the region of MI, right, left and posterior segments of the investigated hearts were assessed. Sham-operated MI rats (n = 6) showed the expected delay of total activation time in MI segments. In contrast, the total activation time was normal in EHT grafted hearts (n = 6), indicating undelayed anterograde coupling of EHTs to the host myocardium (Fig. 5A). Acidification of the perfusion buffer to pH 6.7 led to uncoupling of EHTs from the native myocardium, proving direct but subnormal graft-host coupling (Fig. 5B).


Engineered heart tissue graft derived from somatic cell nuclear transferred embryonic stem cells improve myocardial performance in infarcted rat heart.

Lü S, Li Y, Gao S, Liu S, Wang H, He W, Zhou J, Liu Z, Zhang Y, Lin Q, Duan C, Yang XJ, Wang C - J. Cell. Mol. Med. (2010)

Electrical coupling of EHT grafts to host myocardium. (A) Total activation time on the region of MI, right, left and posterior segments of the investigated hearts. (B) EHT could be uncoupled after acidification of the hearts. * P< 0.05 versus sham-operated MI rats by anova with Mann-Whitney U-test.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822727&req=5

fig05: Electrical coupling of EHT grafts to host myocardium. (A) Total activation time on the region of MI, right, left and posterior segments of the investigated hearts. (B) EHT could be uncoupled after acidification of the hearts. * P< 0.05 versus sham-operated MI rats by anova with Mann-Whitney U-test.
Mentions: We assessed electrical coupling of EHTs to the host myocardium 4 weeks after engraftment by MEA. The total activation time on the region of MI, right, left and posterior segments of the investigated hearts were assessed. Sham-operated MI rats (n = 6) showed the expected delay of total activation time in MI segments. In contrast, the total activation time was normal in EHT grafted hearts (n = 6), indicating undelayed anterograde coupling of EHTs to the host myocardium (Fig. 5A). Acidification of the perfusion buffer to pH 6.7 led to uncoupling of EHTs from the native myocardium, proving direct but subnormal graft-host coupling (Fig. 5B).

Bottom Line: The EHTs, which were derived from NT-ES cells, inte grated and electrically coupled to host myocardium and exerted beneficial effects on the left ventricular function of infarcted rat heart.NT-ES cells can be used as a source of seeding cells for cardiac tissue engineering.Large contractile EHT grafts can be constructed in vitro with the ability to survive after implantation and improve myocardial performance of infarcted rat hearts.

View Article: PubMed Central - PubMed

Affiliation: Department of Tissue Engineering, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences, Beijing, China Laboratory of Oncology, Affiliated Hospital of Academy of Military Medical Sciences, Beijing, China.

Show MeSH
Related in: MedlinePlus