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Ovarian cancer cytoreduction induces changes in T cell population subsets reducing immunosuppression.

Napoletano C, Bellati F, Landi R, Pauselli S, Marchetti C, Visconti V, Sale P, Liberati M, Rughetti A, Frati L, Panici PB, Nuti M - J. Cell. Mol. Med. (2010)

Bottom Line: Primary cytoreduction is able to increase circulating CD4 and CD8 effector cells and decrease CD4 naïve T cells.Surgically induced reduction of the immunosuppressive environment results in an increased capacity of CD8+ T cells to respond to the recall antigens.Debulking plays a beneficial systemic effect by reverting immunosuppression and restoring immunological fitness.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, 'Sapienza' University of Rome, Italy.

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Related in: MedlinePlus

CD4 and CD8 T-cell subsets in pOC (white column), IDS (light grey column) and rOC (dark grey column) patients compared to control (black column), at days 0 and 15. PBMCs were analysed by cytofluorimetry after cell surface labelling with anti-CCR7, anti-CD45RA, anti-CD3 and anti-CD4 or anti-CD8 antibodies. The analysis were performed on CD3+CD4+ (A) and CD3+CD8+ (B) populations after gating on lymphocytes. CD45RA+CCR7+, CD45RA−CCR7+, CD45RA−CCR72, CD45RA+CCR72 corresponding to naïve, central memory, effector memory and terminally differentiated effector cells, respectively. Data are reported as mean of percentage of CD3+CD4+ or CD3+CD8+cells ± S.D.
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fig03: CD4 and CD8 T-cell subsets in pOC (white column), IDS (light grey column) and rOC (dark grey column) patients compared to control (black column), at days 0 and 15. PBMCs were analysed by cytofluorimetry after cell surface labelling with anti-CCR7, anti-CD45RA, anti-CD3 and anti-CD4 or anti-CD8 antibodies. The analysis were performed on CD3+CD4+ (A) and CD3+CD8+ (B) populations after gating on lymphocytes. CD45RA+CCR7+, CD45RA−CCR7+, CD45RA−CCR72, CD45RA+CCR72 corresponding to naïve, central memory, effector memory and terminally differentiated effector cells, respectively. Data are reported as mean of percentage of CD3+CD4+ or CD3+CD8+cells ± S.D.

Mentions: CD4 and CD8 T-cell populations were analysed by cytofluorimetry for the expression of CD45RA and CCR7 markers to evaluate the proportion of naïve (CD45RA+CCR7+), central memory (CD45RA−CCR7+), effector memory (CD45RA−CCR7−) and terminally differentiated effector (CD45RA+CCR7−) subsets [21] (Fig. 3). Figure 3A shows the changes of circulating CD4 T cell after tumour removal. Before surgery, the relative proportion of CD4 effector T cells was significantly higher (P < 0.05) in pOC patients when compared with the control group. There was a significant increase in CD4 effector T cells after surgery (P < 0.05). Before surgery, CD4 naïve T cells were significantly lower in the pOC group as compared to controls. In the pOC group, there was a significant decrease in CD4 naïve T cells after surgery (P < 0.01).


Ovarian cancer cytoreduction induces changes in T cell population subsets reducing immunosuppression.

Napoletano C, Bellati F, Landi R, Pauselli S, Marchetti C, Visconti V, Sale P, Liberati M, Rughetti A, Frati L, Panici PB, Nuti M - J. Cell. Mol. Med. (2010)

CD4 and CD8 T-cell subsets in pOC (white column), IDS (light grey column) and rOC (dark grey column) patients compared to control (black column), at days 0 and 15. PBMCs were analysed by cytofluorimetry after cell surface labelling with anti-CCR7, anti-CD45RA, anti-CD3 and anti-CD4 or anti-CD8 antibodies. The analysis were performed on CD3+CD4+ (A) and CD3+CD8+ (B) populations after gating on lymphocytes. CD45RA+CCR7+, CD45RA−CCR7+, CD45RA−CCR72, CD45RA+CCR72 corresponding to naïve, central memory, effector memory and terminally differentiated effector cells, respectively. Data are reported as mean of percentage of CD3+CD4+ or CD3+CD8+cells ± S.D.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822725&req=5

fig03: CD4 and CD8 T-cell subsets in pOC (white column), IDS (light grey column) and rOC (dark grey column) patients compared to control (black column), at days 0 and 15. PBMCs were analysed by cytofluorimetry after cell surface labelling with anti-CCR7, anti-CD45RA, anti-CD3 and anti-CD4 or anti-CD8 antibodies. The analysis were performed on CD3+CD4+ (A) and CD3+CD8+ (B) populations after gating on lymphocytes. CD45RA+CCR7+, CD45RA−CCR7+, CD45RA−CCR72, CD45RA+CCR72 corresponding to naïve, central memory, effector memory and terminally differentiated effector cells, respectively. Data are reported as mean of percentage of CD3+CD4+ or CD3+CD8+cells ± S.D.
Mentions: CD4 and CD8 T-cell populations were analysed by cytofluorimetry for the expression of CD45RA and CCR7 markers to evaluate the proportion of naïve (CD45RA+CCR7+), central memory (CD45RA−CCR7+), effector memory (CD45RA−CCR7−) and terminally differentiated effector (CD45RA+CCR7−) subsets [21] (Fig. 3). Figure 3A shows the changes of circulating CD4 T cell after tumour removal. Before surgery, the relative proportion of CD4 effector T cells was significantly higher (P < 0.05) in pOC patients when compared with the control group. There was a significant increase in CD4 effector T cells after surgery (P < 0.05). Before surgery, CD4 naïve T cells were significantly lower in the pOC group as compared to controls. In the pOC group, there was a significant decrease in CD4 naïve T cells after surgery (P < 0.01).

Bottom Line: Primary cytoreduction is able to increase circulating CD4 and CD8 effector cells and decrease CD4 naïve T cells.Surgically induced reduction of the immunosuppressive environment results in an increased capacity of CD8+ T cells to respond to the recall antigens.Debulking plays a beneficial systemic effect by reverting immunosuppression and restoring immunological fitness.

View Article: PubMed Central - PubMed

Affiliation: Department of Experimental Medicine, 'Sapienza' University of Rome, Italy.

Show MeSH
Related in: MedlinePlus