The role of dorsal root ganglia activation and brain-derived neurotrophic factor in multiple sclerosis.
Bottom Line: BDNF protein expression in the spinal cord was significantly increased at 12 dpi, and maintained at 15 dpi.However, there was no significant change in mRNA levels.The anterograde transport of DRG-derived BDNF to the spinal cord may have potential implications in facilitating central myelin repair and neuroprotection.
Affiliation: Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba, Canada.Show MeSH
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Mentions: Based on our model of MS induction , we hypothesized that BDNF expression is induced in the DRG and translocated to the dorsal horn along the dorsal roots. To test our hypothesis we assessed the presence of BDNF in the dorsal root in the EAE animals. We show markedly increased BDNF immunoreactivity in the dorsal roots of active EAE animals compared to NC (Fig. 4A). Several previous studies have shown that BDNF is anterogradely transported in vesicles, from the neuronal cell body along the axon to the synapse [27, 28]. Vesicles are moved around in the cell along the cytoskeleton, controlled by motor proteins, the kinesin and dynein protein families . Kinesin has been shown to regulate the anterograde transport of BDNF from the neuronal cell body to the synapse [30, 31]. We demonstrate anterograde transport of BDNF along the dorsal roots, using co-localization of BDNF with the motor protein kinesin . Kinesin-BDNF double immunohistochemistry showed co-localization of the two proteins throughout the dorsal root, and dorsal root entry zone which indicates that BDNF is anterogradely transported to dorsal horn from DRG (Fig. 5B).
Affiliation: Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba, Canada.