Involvement of COX-2/PGE2 signalling in hypoxia-induced angiogenic response in endothelial cells.
Bottom Line: The results demonstrated that short-term hypoxic treatment enhanced HUVECs proliferation, migration, tube formation, significantly up-regulated COX-2, VEGF, AQP1 mRNA level, protein expression and promoted PGE(2) , VEGF release.Exogenous PGE(2) augments the effects of hypoxia on HUVECs, and partially reversed the inhibitory effects of NS398 on HUVECs proliferation and angiogenic capability.Short-term hypoxic treatment enhanced angiogenic capability of ECs, and COX-2/PGE(2) signalling may play a critical role in the biological response of ECs to hypoxia.
Affiliation: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu, China.Show MeSH
Related in: MedlinePlus
Mentions: In order to better understand the effect of hypoxia on the function of HUVECs, we carried out real-time quantitative PCR and Western blot experiments to measure COX-2 mRNA level and protein expression of HUVECs with different periods of hypoxic exposure. The results showed that COX-2 mRNA was at very low levels in unstimulated cells, early minutely induced by 1.35 folds at 1 hr after hypoxic treatment, and reached top at 3 hrs by 2.67 folds, then afterwards declined to level below 2 folds and remained higher than the untreated control group (P < 0.05) (Fig. 5A). Similarly, COX-2 protein expression also increased significantly at 1 hr and maximized by 1.34 folds at 3 hrs, thereafter declined to levels significantly lower than control (P < 0.05) (Fig. 5B).
Affiliation: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu, China.