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Involvement of COX-2/PGE2 signalling in hypoxia-induced angiogenic response in endothelial cells.

Zhao L, Wu Y, Xu Z, Wang H, Zhao Z, Li Y, Yang P, Wei X - J. Cell. Mol. Med. (2012)

Bottom Line: The results demonstrated that short-term hypoxic treatment enhanced HUVECs proliferation, migration, tube formation, significantly up-regulated COX-2, VEGF, AQP1 mRNA level, protein expression and promoted PGE(2) , VEGF release.Exogenous PGE(2) augments the effects of hypoxia on HUVECs, and partially reversed the inhibitory effects of NS398 on HUVECs proliferation and angiogenic capability.Short-term hypoxic treatment enhanced angiogenic capability of ECs, and COX-2/PGE(2) signalling may play a critical role in the biological response of ECs to hypoxia.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu, China.

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COX-2 mRNA level, protein expression and PGE2 accumulation in supernatant under hypoxic exposure. (A) The mRNA levels of COX-2 at different hypoxic time points. 2−ΔΔCt values were obtained by real-time RT-PCR analysis using GAPDH transcripts for the normalization. (B) The protein expression of COX-2 at different hypoxic time points by Western blotting analysis, the blots and the bar chart below are in one label. (C) Quantification of PGE2 by ELISA assay in supernatant of HUVECs after various periods of hypoxic treatment, the data are expressed in concentration of PGE2. *P < 0.05 versus control group.
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fig05: COX-2 mRNA level, protein expression and PGE2 accumulation in supernatant under hypoxic exposure. (A) The mRNA levels of COX-2 at different hypoxic time points. 2−ΔΔCt values were obtained by real-time RT-PCR analysis using GAPDH transcripts for the normalization. (B) The protein expression of COX-2 at different hypoxic time points by Western blotting analysis, the blots and the bar chart below are in one label. (C) Quantification of PGE2 by ELISA assay in supernatant of HUVECs after various periods of hypoxic treatment, the data are expressed in concentration of PGE2. *P < 0.05 versus control group.

Mentions: In order to better understand the effect of hypoxia on the function of HUVECs, we carried out real-time quantitative PCR and Western blot experiments to measure COX-2 mRNA level and protein expression of HUVECs with different periods of hypoxic exposure. The results showed that COX-2 mRNA was at very low levels in unstimulated cells, early minutely induced by 1.35 folds at 1 hr after hypoxic treatment, and reached top at 3 hrs by 2.67 folds, then afterwards declined to level below 2 folds and remained higher than the untreated control group (P < 0.05) (Fig. 5A). Similarly, COX-2 protein expression also increased significantly at 1 hr and maximized by 1.34 folds at 3 hrs, thereafter declined to levels significantly lower than control (P < 0.05) (Fig. 5B).


Involvement of COX-2/PGE2 signalling in hypoxia-induced angiogenic response in endothelial cells.

Zhao L, Wu Y, Xu Z, Wang H, Zhao Z, Li Y, Yang P, Wei X - J. Cell. Mol. Med. (2012)

COX-2 mRNA level, protein expression and PGE2 accumulation in supernatant under hypoxic exposure. (A) The mRNA levels of COX-2 at different hypoxic time points. 2−ΔΔCt values were obtained by real-time RT-PCR analysis using GAPDH transcripts for the normalization. (B) The protein expression of COX-2 at different hypoxic time points by Western blotting analysis, the blots and the bar chart below are in one label. (C) Quantification of PGE2 by ELISA assay in supernatant of HUVECs after various periods of hypoxic treatment, the data are expressed in concentration of PGE2. *P < 0.05 versus control group.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822696&req=5

fig05: COX-2 mRNA level, protein expression and PGE2 accumulation in supernatant under hypoxic exposure. (A) The mRNA levels of COX-2 at different hypoxic time points. 2−ΔΔCt values were obtained by real-time RT-PCR analysis using GAPDH transcripts for the normalization. (B) The protein expression of COX-2 at different hypoxic time points by Western blotting analysis, the blots and the bar chart below are in one label. (C) Quantification of PGE2 by ELISA assay in supernatant of HUVECs after various periods of hypoxic treatment, the data are expressed in concentration of PGE2. *P < 0.05 versus control group.
Mentions: In order to better understand the effect of hypoxia on the function of HUVECs, we carried out real-time quantitative PCR and Western blot experiments to measure COX-2 mRNA level and protein expression of HUVECs with different periods of hypoxic exposure. The results showed that COX-2 mRNA was at very low levels in unstimulated cells, early minutely induced by 1.35 folds at 1 hr after hypoxic treatment, and reached top at 3 hrs by 2.67 folds, then afterwards declined to level below 2 folds and remained higher than the untreated control group (P < 0.05) (Fig. 5A). Similarly, COX-2 protein expression also increased significantly at 1 hr and maximized by 1.34 folds at 3 hrs, thereafter declined to levels significantly lower than control (P < 0.05) (Fig. 5B).

Bottom Line: The results demonstrated that short-term hypoxic treatment enhanced HUVECs proliferation, migration, tube formation, significantly up-regulated COX-2, VEGF, AQP1 mRNA level, protein expression and promoted PGE(2) , VEGF release.Exogenous PGE(2) augments the effects of hypoxia on HUVECs, and partially reversed the inhibitory effects of NS398 on HUVECs proliferation and angiogenic capability.Short-term hypoxic treatment enhanced angiogenic capability of ECs, and COX-2/PGE(2) signalling may play a critical role in the biological response of ECs to hypoxia.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu, China.

Show MeSH
Related in: MedlinePlus