Limits...
Involvement of COX-2/PGE2 signalling in hypoxia-induced angiogenic response in endothelial cells.

Zhao L, Wu Y, Xu Z, Wang H, Zhao Z, Li Y, Yang P, Wei X - J. Cell. Mol. Med. (2012)

Bottom Line: The results demonstrated that short-term hypoxic treatment enhanced HUVECs proliferation, migration, tube formation, significantly up-regulated COX-2, VEGF, AQP1 mRNA level, protein expression and promoted PGE(2) , VEGF release.Exogenous PGE(2) augments the effects of hypoxia on HUVECs, and partially reversed the inhibitory effects of NS398 on HUVECs proliferation and angiogenic capability.Short-term hypoxic treatment enhanced angiogenic capability of ECs, and COX-2/PGE(2) signalling may play a critical role in the biological response of ECs to hypoxia.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu, China.

Show MeSH

Related in: MedlinePlus

Characterization of HUVECs by morphological observation and immunocytochemical staining. (A) The cells exhibited typical cobblestone morphology on reaching confluence, insert with higher magnification of ×200. (B and C) Immunocytochemical stained with factor VIII-related antigen and CD31 antibody, respectively, the immunoreactive positive cells were stained brownish-yellow, insert with higher magnification of ×400 confirming brownish-yellow stained cytoplasm. Scale bars: 100 μm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3822696&req=5

fig02: Characterization of HUVECs by morphological observation and immunocytochemical staining. (A) The cells exhibited typical cobblestone morphology on reaching confluence, insert with higher magnification of ×200. (B and C) Immunocytochemical stained with factor VIII-related antigen and CD31 antibody, respectively, the immunoreactive positive cells were stained brownish-yellow, insert with higher magnification of ×400 confirming brownish-yellow stained cytoplasm. Scale bars: 100 μm.

Mentions: The cells exhibited typical cobblestone morphology on reaching confluence (Fig. 2A), and immunocytochemical staining showed that they expressed factor VIII-related antigen and CD31 (Fig. 2B and C), which initially suggested that the cells are ECs.


Involvement of COX-2/PGE2 signalling in hypoxia-induced angiogenic response in endothelial cells.

Zhao L, Wu Y, Xu Z, Wang H, Zhao Z, Li Y, Yang P, Wei X - J. Cell. Mol. Med. (2012)

Characterization of HUVECs by morphological observation and immunocytochemical staining. (A) The cells exhibited typical cobblestone morphology on reaching confluence, insert with higher magnification of ×200. (B and C) Immunocytochemical stained with factor VIII-related antigen and CD31 antibody, respectively, the immunoreactive positive cells were stained brownish-yellow, insert with higher magnification of ×400 confirming brownish-yellow stained cytoplasm. Scale bars: 100 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822696&req=5

fig02: Characterization of HUVECs by morphological observation and immunocytochemical staining. (A) The cells exhibited typical cobblestone morphology on reaching confluence, insert with higher magnification of ×200. (B and C) Immunocytochemical stained with factor VIII-related antigen and CD31 antibody, respectively, the immunoreactive positive cells were stained brownish-yellow, insert with higher magnification of ×400 confirming brownish-yellow stained cytoplasm. Scale bars: 100 μm.
Mentions: The cells exhibited typical cobblestone morphology on reaching confluence (Fig. 2A), and immunocytochemical staining showed that they expressed factor VIII-related antigen and CD31 (Fig. 2B and C), which initially suggested that the cells are ECs.

Bottom Line: The results demonstrated that short-term hypoxic treatment enhanced HUVECs proliferation, migration, tube formation, significantly up-regulated COX-2, VEGF, AQP1 mRNA level, protein expression and promoted PGE(2) , VEGF release.Exogenous PGE(2) augments the effects of hypoxia on HUVECs, and partially reversed the inhibitory effects of NS398 on HUVECs proliferation and angiogenic capability.Short-term hypoxic treatment enhanced angiogenic capability of ECs, and COX-2/PGE(2) signalling may play a critical role in the biological response of ECs to hypoxia.

View Article: PubMed Central - PubMed

Affiliation: State Key Laboratory of Oral Diseases, Department of Orthodontics, West China College of Stomatology, Sichuan University, Chengdu, China.

Show MeSH
Related in: MedlinePlus