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Human umbilical cord mesenchymal stem cells suppress breast cancer tumourigenesis through direct cell-cell contact and internalization.

Chao KC, Yang HT, Chen MW - J. Cell. Mol. Med. (2012)

Bottom Line: Also, treatment with HUMSC injection was efficacious in both in situ and metastatic breast cancers in the animal models.Since HUMSCs were proved to efficaciously suppress breast cancer tumourigenesis both in vitro and in vivo, it is our expectation that treatment with HUMSCs can be a viable therapy for breast cancer in the near future.In addition, we share a new point of view on the role of HUMSCs in foetal development during pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan.

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MDA-MB231 apoptosis following binding or formation of cell-in-cell structure with selected HUMSC. (A) Confocal microscopy demonstrated the cell-in-cell structure of selected HUMSC (red) internalized within MDA-MB231 (green) after co-culture. Simultaneous presentation of strong red fluorescence came from HUMSC within the region of green fluorescence delineated by MDA-MB231 suggested that some substance from HUMSC had been intermixed within MDA-MB231, and the MDA-MB231 was stained positively by TUNEL (top panels). Binding of HS68 (red) with MDA-MB231 (green) was rare, and no cells were TUNEL-positive (middle panels). The cell-in-cell structure of WI38 (red) internalized within MDA-MB231 (green) was stained negatively by TUNEL (bottom panels). Scale bars: 10 μm. (B) The percentage of TUNEL-positive cells after co-culture for 3 days was 17.66 ± 0.58% of co-cultured MDA-MB231 with selected HUMSC, but only 0.61 ± 0.31% of co-cultured MDA-MB231 with WI38 and 0 ± 0% of co-cultured MDA-MB231 with HS68 was TUNEL-positive. Data are means ± SEM. (C) Using flow cytometry, the number of cells with simply red (Q1), simply green (Q4) and dual (red + green) fluorescence (Q2) was measured. The percentage of cells with dual fluorescence was 49.2%, 9.9% and 79.2% after co-culture of MDA-MB231 with selected HUMSC, HS68 and WI38, respectively. (D) The binding rate of each cell population.
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fig02: MDA-MB231 apoptosis following binding or formation of cell-in-cell structure with selected HUMSC. (A) Confocal microscopy demonstrated the cell-in-cell structure of selected HUMSC (red) internalized within MDA-MB231 (green) after co-culture. Simultaneous presentation of strong red fluorescence came from HUMSC within the region of green fluorescence delineated by MDA-MB231 suggested that some substance from HUMSC had been intermixed within MDA-MB231, and the MDA-MB231 was stained positively by TUNEL (top panels). Binding of HS68 (red) with MDA-MB231 (green) was rare, and no cells were TUNEL-positive (middle panels). The cell-in-cell structure of WI38 (red) internalized within MDA-MB231 (green) was stained negatively by TUNEL (bottom panels). Scale bars: 10 μm. (B) The percentage of TUNEL-positive cells after co-culture for 3 days was 17.66 ± 0.58% of co-cultured MDA-MB231 with selected HUMSC, but only 0.61 ± 0.31% of co-cultured MDA-MB231 with WI38 and 0 ± 0% of co-cultured MDA-MB231 with HS68 was TUNEL-positive. Data are means ± SEM. (C) Using flow cytometry, the number of cells with simply red (Q1), simply green (Q4) and dual (red + green) fluorescence (Q2) was measured. The percentage of cells with dual fluorescence was 49.2%, 9.9% and 79.2% after co-culture of MDA-MB231 with selected HUMSC, HS68 and WI38, respectively. (D) The binding rate of each cell population.

Mentions: The cell-in-cell structure of selected HUMSC internalized within MDA MB-23l was manifested on confocal microscopy (Fig. 1D), with substance from HUMSC intermixed within MDA-MB231 noted (Fig. 2A). Although less dominant, it was also found that in a few cases, some substance from MDA-MB231 had been intermixed within HUMSC (Fig. S2A), implying that infusion of substance from MDA-MB231 into HUMSC also occurred during their interaction.


Human umbilical cord mesenchymal stem cells suppress breast cancer tumourigenesis through direct cell-cell contact and internalization.

Chao KC, Yang HT, Chen MW - J. Cell. Mol. Med. (2012)

MDA-MB231 apoptosis following binding or formation of cell-in-cell structure with selected HUMSC. (A) Confocal microscopy demonstrated the cell-in-cell structure of selected HUMSC (red) internalized within MDA-MB231 (green) after co-culture. Simultaneous presentation of strong red fluorescence came from HUMSC within the region of green fluorescence delineated by MDA-MB231 suggested that some substance from HUMSC had been intermixed within MDA-MB231, and the MDA-MB231 was stained positively by TUNEL (top panels). Binding of HS68 (red) with MDA-MB231 (green) was rare, and no cells were TUNEL-positive (middle panels). The cell-in-cell structure of WI38 (red) internalized within MDA-MB231 (green) was stained negatively by TUNEL (bottom panels). Scale bars: 10 μm. (B) The percentage of TUNEL-positive cells after co-culture for 3 days was 17.66 ± 0.58% of co-cultured MDA-MB231 with selected HUMSC, but only 0.61 ± 0.31% of co-cultured MDA-MB231 with WI38 and 0 ± 0% of co-cultured MDA-MB231 with HS68 was TUNEL-positive. Data are means ± SEM. (C) Using flow cytometry, the number of cells with simply red (Q1), simply green (Q4) and dual (red + green) fluorescence (Q2) was measured. The percentage of cells with dual fluorescence was 49.2%, 9.9% and 79.2% after co-culture of MDA-MB231 with selected HUMSC, HS68 and WI38, respectively. (D) The binding rate of each cell population.
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fig02: MDA-MB231 apoptosis following binding or formation of cell-in-cell structure with selected HUMSC. (A) Confocal microscopy demonstrated the cell-in-cell structure of selected HUMSC (red) internalized within MDA-MB231 (green) after co-culture. Simultaneous presentation of strong red fluorescence came from HUMSC within the region of green fluorescence delineated by MDA-MB231 suggested that some substance from HUMSC had been intermixed within MDA-MB231, and the MDA-MB231 was stained positively by TUNEL (top panels). Binding of HS68 (red) with MDA-MB231 (green) was rare, and no cells were TUNEL-positive (middle panels). The cell-in-cell structure of WI38 (red) internalized within MDA-MB231 (green) was stained negatively by TUNEL (bottom panels). Scale bars: 10 μm. (B) The percentage of TUNEL-positive cells after co-culture for 3 days was 17.66 ± 0.58% of co-cultured MDA-MB231 with selected HUMSC, but only 0.61 ± 0.31% of co-cultured MDA-MB231 with WI38 and 0 ± 0% of co-cultured MDA-MB231 with HS68 was TUNEL-positive. Data are means ± SEM. (C) Using flow cytometry, the number of cells with simply red (Q1), simply green (Q4) and dual (red + green) fluorescence (Q2) was measured. The percentage of cells with dual fluorescence was 49.2%, 9.9% and 79.2% after co-culture of MDA-MB231 with selected HUMSC, HS68 and WI38, respectively. (D) The binding rate of each cell population.
Mentions: The cell-in-cell structure of selected HUMSC internalized within MDA MB-23l was manifested on confocal microscopy (Fig. 1D), with substance from HUMSC intermixed within MDA-MB231 noted (Fig. 2A). Although less dominant, it was also found that in a few cases, some substance from MDA-MB231 had been intermixed within HUMSC (Fig. S2A), implying that infusion of substance from MDA-MB231 into HUMSC also occurred during their interaction.

Bottom Line: Also, treatment with HUMSC injection was efficacious in both in situ and metastatic breast cancers in the animal models.Since HUMSCs were proved to efficaciously suppress breast cancer tumourigenesis both in vitro and in vivo, it is our expectation that treatment with HUMSCs can be a viable therapy for breast cancer in the near future.In addition, we share a new point of view on the role of HUMSCs in foetal development during pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan.

Show MeSH
Related in: MedlinePlus