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Human umbilical cord mesenchymal stem cells suppress breast cancer tumourigenesis through direct cell-cell contact and internalization.

Chao KC, Yang HT, Chen MW - J. Cell. Mol. Med. (2012)

Bottom Line: Also, treatment with HUMSC injection was efficacious in both in situ and metastatic breast cancers in the animal models.Since HUMSCs were proved to efficaciously suppress breast cancer tumourigenesis both in vitro and in vivo, it is our expectation that treatment with HUMSCs can be a viable therapy for breast cancer in the near future.In addition, we share a new point of view on the role of HUMSCs in foetal development during pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan.

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Related in: MedlinePlus

Effect and mechanism of selected HUMSC to suppress MDA-MB231. (A) Using Cellomics ArrayScan, the number of MDA-MB231 decreased slightly in five of the six co-cultured wells (HUMSC), whereas in one of the six wells (S-HUMSC), the number of MDA-MB231 dramatically decreased. The somehow more ‘active’ HUMSCs with better ability to suppress tumourigenesis were selected for further experiments. Data of HUMSC are means ± SEM (n = 5). (B) Fluoroscopic images from time-lapse analysis after co-culture showed the selected HUMSC (red) and MDA-MB231 (green) adhered on contact, and then the HUMSC infused some of its substance into MDA-MB231. (C) Fluoroscopic images from time-lapse analysis after co-culture showed the selected HUMSC (red) contacted with MDA-MB231 (green) and then internalized into MDA-MB231 to form a cell-in-cell structure. The MDA-MB231 shrank following their subsequent separation. (D) On confocal microscopy, simultaneous presentation of MDA-MB231 (green) and HUMSC (red) at the same site at a plane manifested the cell-in-cell structure of selected HUMSC internalized within MDA-MB231. Scale bars: 15 μm. (E) After co-culture of MDA-MB231 (green) with selected HUMSC (red), cells with dual fluorescence (red + green) were isolated and cultured for three more days. Only the red fluorescence was still visible on fluoroscopy. Scale bars: 100 μm.
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fig01: Effect and mechanism of selected HUMSC to suppress MDA-MB231. (A) Using Cellomics ArrayScan, the number of MDA-MB231 decreased slightly in five of the six co-cultured wells (HUMSC), whereas in one of the six wells (S-HUMSC), the number of MDA-MB231 dramatically decreased. The somehow more ‘active’ HUMSCs with better ability to suppress tumourigenesis were selected for further experiments. Data of HUMSC are means ± SEM (n = 5). (B) Fluoroscopic images from time-lapse analysis after co-culture showed the selected HUMSC (red) and MDA-MB231 (green) adhered on contact, and then the HUMSC infused some of its substance into MDA-MB231. (C) Fluoroscopic images from time-lapse analysis after co-culture showed the selected HUMSC (red) contacted with MDA-MB231 (green) and then internalized into MDA-MB231 to form a cell-in-cell structure. The MDA-MB231 shrank following their subsequent separation. (D) On confocal microscopy, simultaneous presentation of MDA-MB231 (green) and HUMSC (red) at the same site at a plane manifested the cell-in-cell structure of selected HUMSC internalized within MDA-MB231. Scale bars: 15 μm. (E) After co-culture of MDA-MB231 (green) with selected HUMSC (red), cells with dual fluorescence (red + green) were isolated and cultured for three more days. Only the red fluorescence was still visible on fluoroscopy. Scale bars: 100 μm.

Mentions: As a first step, we used Cellomics ArrayScan to observe how MDA-MB231 responds to HUMSC. In most of the co-cultured wells, the number of MDA-MB231 decreased slightly, whereas in some wells, the number of MDA-MB231 decreased dramatically. The frequency of the more ‘active’ HUMSCs versus the total number of sample tested was 1/6 (16.67%) (Fig. 1A). The HUMSCs were found to have the ability to suppress MDA-MB231 cell growth to different degrees, so the somehow more ‘active’ HUMSCs with better ability to suppress tumourigenesis were selected for further experiments.


Human umbilical cord mesenchymal stem cells suppress breast cancer tumourigenesis through direct cell-cell contact and internalization.

Chao KC, Yang HT, Chen MW - J. Cell. Mol. Med. (2012)

Effect and mechanism of selected HUMSC to suppress MDA-MB231. (A) Using Cellomics ArrayScan, the number of MDA-MB231 decreased slightly in five of the six co-cultured wells (HUMSC), whereas in one of the six wells (S-HUMSC), the number of MDA-MB231 dramatically decreased. The somehow more ‘active’ HUMSCs with better ability to suppress tumourigenesis were selected for further experiments. Data of HUMSC are means ± SEM (n = 5). (B) Fluoroscopic images from time-lapse analysis after co-culture showed the selected HUMSC (red) and MDA-MB231 (green) adhered on contact, and then the HUMSC infused some of its substance into MDA-MB231. (C) Fluoroscopic images from time-lapse analysis after co-culture showed the selected HUMSC (red) contacted with MDA-MB231 (green) and then internalized into MDA-MB231 to form a cell-in-cell structure. The MDA-MB231 shrank following their subsequent separation. (D) On confocal microscopy, simultaneous presentation of MDA-MB231 (green) and HUMSC (red) at the same site at a plane manifested the cell-in-cell structure of selected HUMSC internalized within MDA-MB231. Scale bars: 15 μm. (E) After co-culture of MDA-MB231 (green) with selected HUMSC (red), cells with dual fluorescence (red + green) were isolated and cultured for three more days. Only the red fluorescence was still visible on fluoroscopy. Scale bars: 100 μm.
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Related In: Results  -  Collection

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fig01: Effect and mechanism of selected HUMSC to suppress MDA-MB231. (A) Using Cellomics ArrayScan, the number of MDA-MB231 decreased slightly in five of the six co-cultured wells (HUMSC), whereas in one of the six wells (S-HUMSC), the number of MDA-MB231 dramatically decreased. The somehow more ‘active’ HUMSCs with better ability to suppress tumourigenesis were selected for further experiments. Data of HUMSC are means ± SEM (n = 5). (B) Fluoroscopic images from time-lapse analysis after co-culture showed the selected HUMSC (red) and MDA-MB231 (green) adhered on contact, and then the HUMSC infused some of its substance into MDA-MB231. (C) Fluoroscopic images from time-lapse analysis after co-culture showed the selected HUMSC (red) contacted with MDA-MB231 (green) and then internalized into MDA-MB231 to form a cell-in-cell structure. The MDA-MB231 shrank following their subsequent separation. (D) On confocal microscopy, simultaneous presentation of MDA-MB231 (green) and HUMSC (red) at the same site at a plane manifested the cell-in-cell structure of selected HUMSC internalized within MDA-MB231. Scale bars: 15 μm. (E) After co-culture of MDA-MB231 (green) with selected HUMSC (red), cells with dual fluorescence (red + green) were isolated and cultured for three more days. Only the red fluorescence was still visible on fluoroscopy. Scale bars: 100 μm.
Mentions: As a first step, we used Cellomics ArrayScan to observe how MDA-MB231 responds to HUMSC. In most of the co-cultured wells, the number of MDA-MB231 decreased slightly, whereas in some wells, the number of MDA-MB231 decreased dramatically. The frequency of the more ‘active’ HUMSCs versus the total number of sample tested was 1/6 (16.67%) (Fig. 1A). The HUMSCs were found to have the ability to suppress MDA-MB231 cell growth to different degrees, so the somehow more ‘active’ HUMSCs with better ability to suppress tumourigenesis were selected for further experiments.

Bottom Line: Also, treatment with HUMSC injection was efficacious in both in situ and metastatic breast cancers in the animal models.Since HUMSCs were proved to efficaciously suppress breast cancer tumourigenesis both in vitro and in vivo, it is our expectation that treatment with HUMSCs can be a viable therapy for breast cancer in the near future.In addition, we share a new point of view on the role of HUMSCs in foetal development during pregnancy.

View Article: PubMed Central - PubMed

Affiliation: Genomics Research Center, Academia Sinica, Taipei, Taiwan.

Show MeSH
Related in: MedlinePlus