Limits...
Erythropoietin attenuates the sequels of ischaemic spinal cord injury with enhanced recruitment of CD34+ cells in mice.

Hirano K, Wagner K, Mark P, Pittermann E, Gäbel R, Furlani D, Li W, Vollmar B, Yamada T, Steinhoff G, Ma N - J. Cell. Mol. Med. (2012)

Bottom Line: Immunohistochemistry was used to detect CD34(+) cells and the expression of brain-derived neurotrophic factor and vascular endothelial growth factor.Erythropoietin demonstrated neuroprotective effects in the ischaemic spinal cord, improving neurological function and attenuating motor neuron loss.These effects may have been mediated by recruited CD34(+) cells, and enhanced expression of brain-derived neurotrophic factor and vascular endothelial growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

Show MeSH

Related in: MedlinePlus

Representative pictures of CD34+ cells in ventral grey matter (A) and enlargement of boxed area (B). Blue: nuclei and red: CD34. (A) Scale bar = 25 μm; (B) Scale bar = 10 μm. The number of CD34+ cells in the lumbar spinal cord after 7-min. (C) and 9-min. SCI (D). CD34+ cells were more abundantly recruited in the spinal cord in groups 7E-severe and 9E-severe than those in groups 7C-severe and 9C-severe at day 2 (C and D, *P < 0.05 with Bonferroni correction). CD34+ cells were not apparent in groups 7C-mild, 7E-mild, 9C-mild and 9E-mild. Representative pictures of immunofluorescence for BDNF in ventral grey matter from groups 9E-severe (E) and 9C-severe (F) at day 7. Blue: nuclei and green: BDNF. BDNF expression in group 9E-severe (E) was more evident than that in group 9C-severe (F). Scale bar = 25 μm. Representative pictures of VEGF expression in the left ventrolateral white matter of the spinal cord from groups 9E-severe (G) and 9C-severe (H) at day 7. VEGF was detectable in the white matter of group 9E-severe and vacuolization in white matter was limited (G). In contrast, no VEGF expression and advanced vacuolization was apparent in the white matter of group 9C-severe (H). Scale bar = 50 μm.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC3822692&req=5

fig05: Representative pictures of CD34+ cells in ventral grey matter (A) and enlargement of boxed area (B). Blue: nuclei and red: CD34. (A) Scale bar = 25 μm; (B) Scale bar = 10 μm. The number of CD34+ cells in the lumbar spinal cord after 7-min. (C) and 9-min. SCI (D). CD34+ cells were more abundantly recruited in the spinal cord in groups 7E-severe and 9E-severe than those in groups 7C-severe and 9C-severe at day 2 (C and D, *P < 0.05 with Bonferroni correction). CD34+ cells were not apparent in groups 7C-mild, 7E-mild, 9C-mild and 9E-mild. Representative pictures of immunofluorescence for BDNF in ventral grey matter from groups 9E-severe (E) and 9C-severe (F) at day 7. Blue: nuclei and green: BDNF. BDNF expression in group 9E-severe (E) was more evident than that in group 9C-severe (F). Scale bar = 25 μm. Representative pictures of VEGF expression in the left ventrolateral white matter of the spinal cord from groups 9E-severe (G) and 9C-severe (H) at day 7. VEGF was detectable in the white matter of group 9E-severe and vacuolization in white matter was limited (G). In contrast, no VEGF expression and advanced vacuolization was apparent in the white matter of group 9C-severe (H). Scale bar = 50 μm.

Mentions: CD34+ cells could be recognized in cross sections, frequently in grey matter of the lumbar spinal cord in every group (Fig. 5A and B). The number of CD34+ cells in group 7E-severe was higher than that of group 7C-severe at day 2 (28.3 ± 9.5 versus 13.8 ± 5.1, respectively, P < 0.05 with Bonferroni correction), and that of group 9E-severe was also higher than that of group 9C-severe at day 2 (44.8 ± 22.4 versus 22.7 ± 11.8, respectively, P < 0.05 with Bonferroni correction, Fig. 5C and D). No significant difference was observed in CD34+ cells from groups 7C-mild, 7E-mild, 9C-mild and 9E-mild. Expression of BDNF was distributed in the ventral grey matter of groups 7E-severe and 9E-severe, whereas expression was rarely detected in groups 7C-severe and 9C-severe (Fig. 5E and F). Similarly, expression of VEGF could be detected only in the ventral or ventrolateral white matter of the spinal cord in groups 7E-severe and 9E-severe, but not in groups 7C-severe and 9C-severe (Fig. 5G and H).


Erythropoietin attenuates the sequels of ischaemic spinal cord injury with enhanced recruitment of CD34+ cells in mice.

Hirano K, Wagner K, Mark P, Pittermann E, Gäbel R, Furlani D, Li W, Vollmar B, Yamada T, Steinhoff G, Ma N - J. Cell. Mol. Med. (2012)

Representative pictures of CD34+ cells in ventral grey matter (A) and enlargement of boxed area (B). Blue: nuclei and red: CD34. (A) Scale bar = 25 μm; (B) Scale bar = 10 μm. The number of CD34+ cells in the lumbar spinal cord after 7-min. (C) and 9-min. SCI (D). CD34+ cells were more abundantly recruited in the spinal cord in groups 7E-severe and 9E-severe than those in groups 7C-severe and 9C-severe at day 2 (C and D, *P < 0.05 with Bonferroni correction). CD34+ cells were not apparent in groups 7C-mild, 7E-mild, 9C-mild and 9E-mild. Representative pictures of immunofluorescence for BDNF in ventral grey matter from groups 9E-severe (E) and 9C-severe (F) at day 7. Blue: nuclei and green: BDNF. BDNF expression in group 9E-severe (E) was more evident than that in group 9C-severe (F). Scale bar = 25 μm. Representative pictures of VEGF expression in the left ventrolateral white matter of the spinal cord from groups 9E-severe (G) and 9C-severe (H) at day 7. VEGF was detectable in the white matter of group 9E-severe and vacuolization in white matter was limited (G). In contrast, no VEGF expression and advanced vacuolization was apparent in the white matter of group 9C-severe (H). Scale bar = 50 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC3822692&req=5

fig05: Representative pictures of CD34+ cells in ventral grey matter (A) and enlargement of boxed area (B). Blue: nuclei and red: CD34. (A) Scale bar = 25 μm; (B) Scale bar = 10 μm. The number of CD34+ cells in the lumbar spinal cord after 7-min. (C) and 9-min. SCI (D). CD34+ cells were more abundantly recruited in the spinal cord in groups 7E-severe and 9E-severe than those in groups 7C-severe and 9C-severe at day 2 (C and D, *P < 0.05 with Bonferroni correction). CD34+ cells were not apparent in groups 7C-mild, 7E-mild, 9C-mild and 9E-mild. Representative pictures of immunofluorescence for BDNF in ventral grey matter from groups 9E-severe (E) and 9C-severe (F) at day 7. Blue: nuclei and green: BDNF. BDNF expression in group 9E-severe (E) was more evident than that in group 9C-severe (F). Scale bar = 25 μm. Representative pictures of VEGF expression in the left ventrolateral white matter of the spinal cord from groups 9E-severe (G) and 9C-severe (H) at day 7. VEGF was detectable in the white matter of group 9E-severe and vacuolization in white matter was limited (G). In contrast, no VEGF expression and advanced vacuolization was apparent in the white matter of group 9C-severe (H). Scale bar = 50 μm.
Mentions: CD34+ cells could be recognized in cross sections, frequently in grey matter of the lumbar spinal cord in every group (Fig. 5A and B). The number of CD34+ cells in group 7E-severe was higher than that of group 7C-severe at day 2 (28.3 ± 9.5 versus 13.8 ± 5.1, respectively, P < 0.05 with Bonferroni correction), and that of group 9E-severe was also higher than that of group 9C-severe at day 2 (44.8 ± 22.4 versus 22.7 ± 11.8, respectively, P < 0.05 with Bonferroni correction, Fig. 5C and D). No significant difference was observed in CD34+ cells from groups 7C-mild, 7E-mild, 9C-mild and 9E-mild. Expression of BDNF was distributed in the ventral grey matter of groups 7E-severe and 9E-severe, whereas expression was rarely detected in groups 7C-severe and 9C-severe (Fig. 5E and F). Similarly, expression of VEGF could be detected only in the ventral or ventrolateral white matter of the spinal cord in groups 7E-severe and 9E-severe, but not in groups 7C-severe and 9C-severe (Fig. 5G and H).

Bottom Line: Immunohistochemistry was used to detect CD34(+) cells and the expression of brain-derived neurotrophic factor and vascular endothelial growth factor.Erythropoietin demonstrated neuroprotective effects in the ischaemic spinal cord, improving neurological function and attenuating motor neuron loss.These effects may have been mediated by recruited CD34(+) cells, and enhanced expression of brain-derived neurotrophic factor and vascular endothelial growth factor.

View Article: PubMed Central - PubMed

Affiliation: Department of Cardiac Surgery, University of Rostock, Rostock, Germany.

Show MeSH
Related in: MedlinePlus