Erythropoietin attenuates the sequels of ischaemic spinal cord injury with enhanced recruitment of CD34+ cells in mice.
Bottom Line: Immunohistochemistry was used to detect CD34(+) cells and the expression of brain-derived neurotrophic factor and vascular endothelial growth factor.Erythropoietin demonstrated neuroprotective effects in the ischaemic spinal cord, improving neurological function and attenuating motor neuron loss.These effects may have been mediated by recruited CD34(+) cells, and enhanced expression of brain-derived neurotrophic factor and vascular endothelial growth factor.
Affiliation: Department of Cardiac Surgery, University of Rostock, Rostock, Germany.Show MeSH
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Mentions: CD34+ cells could be recognized in cross sections, frequently in grey matter of the lumbar spinal cord in every group (Fig. 5A and B). The number of CD34+ cells in group 7E-severe was higher than that of group 7C-severe at day 2 (28.3 ± 9.5 versus 13.8 ± 5.1, respectively, P < 0.05 with Bonferroni correction), and that of group 9E-severe was also higher than that of group 9C-severe at day 2 (44.8 ± 22.4 versus 22.7 ± 11.8, respectively, P < 0.05 with Bonferroni correction, Fig. 5C and D). No significant difference was observed in CD34+ cells from groups 7C-mild, 7E-mild, 9C-mild and 9E-mild. Expression of BDNF was distributed in the ventral grey matter of groups 7E-severe and 9E-severe, whereas expression was rarely detected in groups 7C-severe and 9C-severe (Fig. 5E and F). Similarly, expression of VEGF could be detected only in the ventral or ventrolateral white matter of the spinal cord in groups 7E-severe and 9E-severe, but not in groups 7C-severe and 9C-severe (Fig. 5G and H).
Affiliation: Department of Cardiac Surgery, University of Rostock, Rostock, Germany.