Age-related changes in the contractile and passive arterial properties of murine mesenteric small arteries are altered by caveolin-1 knockout.
Bottom Line: Caveolin-1, an integral protein of caveolae, is associated with multiple cardiovascular signalling pathways.As changes in artery structure and function are associated with ageing we have investigated the role of caveolin-1 ablation on age-related changes of small artery contractility and passive mechanical properties.Caveolin-1 ablation at 3 months of age resulted in similar changes in passive arterial properties to those observed with ageing in WT animals.
Affiliation: Cardiovascular Research Group, University of Manchester, Manchester, UK.Show MeSH
Related in: MedlinePlus
Mentions: DNA was extracted in lysis buffer (composition: 50 mM Tris pH8, 100 mM EDTA, 0.5% SDS, 10 mg/ml proteinase K; Promega, Southampton, UK) isopropanol precipitated, washed in ethanol and resuspended in distilled water. WT, heterozygous (Ht) or KO genotype was confirmed by PCR (Fig. 1) using primers specific for the WT (forward: TTTACCGCTTGTTGTCTACGA; reverse: TATCTCTTTCTGCGTGCTGA; product size 240bp) or KO genotype (forward: TATTCTGCCTTCCTGATGATAACTG; reverse: CCTGCGTGCAATCCATCTTGTTCAATG; product size 1500bp).
Affiliation: Cardiovascular Research Group, University of Manchester, Manchester, UK.