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Analysis of microdissected neurons by 18O mass spectrometry reveals altered protein expression in Alzheimer's disease.

Hashimoto M, Bogdanovic N, Nakagawa H, Volkmann I, Aoki M, Winblad B, Sakai J, Tjernberg LO - J. Cell. Mol. Med. (2012)

Bottom Line: These analyses confirmed the altered expression levels, and showed in many AD cases a pathological pattern.The expression levels found by this method showed poor correlation with the neuron-specific analysis.Hence, we conclude that cell-specific proteome analysis reveals differences in the proteome that cannot be detected by bulk analysis.

View Article: PubMed Central - PubMed

Affiliation: Karolinska Institutet and Dainippon Sumitomo Pharma Alzheimer Center, Department of Neurobiology, Care Sciences and Society, NVS, Karolinska Institutet, Huddinge, Sweden.

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Related in: MedlinePlus

NKRF, TP53B and LRP1 staining in CA1 region. (A, B) NKRF in CA1; (C, D) TP53B in CA1; (E, F) TP53B in gyrus dentatus; (G, H) LRP1 in CA1; (A, C, E, G) Controls, case number 2, 3, 3 and 6, respectively. (B, D, F, H) Sporadic AD, case numbers 9, 10, 10 and 9, respectively. These proteins were detected in nucleolus in AD cases. Magnification of originals: 40χ. Magnification of insets: 70χ. Scale bar corresponds to 50 μm.
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fig03: NKRF, TP53B and LRP1 staining in CA1 region. (A, B) NKRF in CA1; (C, D) TP53B in CA1; (E, F) TP53B in gyrus dentatus; (G, H) LRP1 in CA1; (A, C, E, G) Controls, case number 2, 3, 3 and 6, respectively. (B, D, F, H) Sporadic AD, case numbers 9, 10, 10 and 9, respectively. These proteins were detected in nucleolus in AD cases. Magnification of originals: 40χ. Magnification of insets: 70χ. Scale bar corresponds to 50 μm.

Mentions: The expression of NKRF was detected only in pyramidal neurons, and was predominantly limited to the nucleolus with weak positivity in the nucleoplasm and perinuclear membrane in the control case (Fig. 3A). Although intense staining was detectable in the nucleolus in the AD case, the perinuclear and nucleoplasm staining was weaker than in the controls (Fig. 3B). The expression level in CA1 pyramidal neurons was clearly higher than in pyramidal neurons in CA3 and cortex (data not shown).


Analysis of microdissected neurons by 18O mass spectrometry reveals altered protein expression in Alzheimer's disease.

Hashimoto M, Bogdanovic N, Nakagawa H, Volkmann I, Aoki M, Winblad B, Sakai J, Tjernberg LO - J. Cell. Mol. Med. (2012)

NKRF, TP53B and LRP1 staining in CA1 region. (A, B) NKRF in CA1; (C, D) TP53B in CA1; (E, F) TP53B in gyrus dentatus; (G, H) LRP1 in CA1; (A, C, E, G) Controls, case number 2, 3, 3 and 6, respectively. (B, D, F, H) Sporadic AD, case numbers 9, 10, 10 and 9, respectively. These proteins were detected in nucleolus in AD cases. Magnification of originals: 40χ. Magnification of insets: 70χ. Scale bar corresponds to 50 μm.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC3822682&req=5

fig03: NKRF, TP53B and LRP1 staining in CA1 region. (A, B) NKRF in CA1; (C, D) TP53B in CA1; (E, F) TP53B in gyrus dentatus; (G, H) LRP1 in CA1; (A, C, E, G) Controls, case number 2, 3, 3 and 6, respectively. (B, D, F, H) Sporadic AD, case numbers 9, 10, 10 and 9, respectively. These proteins were detected in nucleolus in AD cases. Magnification of originals: 40χ. Magnification of insets: 70χ. Scale bar corresponds to 50 μm.
Mentions: The expression of NKRF was detected only in pyramidal neurons, and was predominantly limited to the nucleolus with weak positivity in the nucleoplasm and perinuclear membrane in the control case (Fig. 3A). Although intense staining was detectable in the nucleolus in the AD case, the perinuclear and nucleoplasm staining was weaker than in the controls (Fig. 3B). The expression level in CA1 pyramidal neurons was clearly higher than in pyramidal neurons in CA3 and cortex (data not shown).

Bottom Line: These analyses confirmed the altered expression levels, and showed in many AD cases a pathological pattern.The expression levels found by this method showed poor correlation with the neuron-specific analysis.Hence, we conclude that cell-specific proteome analysis reveals differences in the proteome that cannot be detected by bulk analysis.

View Article: PubMed Central - PubMed

Affiliation: Karolinska Institutet and Dainippon Sumitomo Pharma Alzheimer Center, Department of Neurobiology, Care Sciences and Society, NVS, Karolinska Institutet, Huddinge, Sweden.

Show MeSH
Related in: MedlinePlus