Analysis of microdissected neurons by 18O mass spectrometry reveals altered protein expression in Alzheimer's disease.
Bottom Line: These analyses confirmed the altered expression levels, and showed in many AD cases a pathological pattern.The expression levels found by this method showed poor correlation with the neuron-specific analysis.Hence, we conclude that cell-specific proteome analysis reveals differences in the proteome that cannot be detected by bulk analysis.
Affiliation: Karolinska Institutet and Dainippon Sumitomo Pharma Alzheimer Center, Department of Neurobiology, Care Sciences and Society, NVS, Karolinska Institutet, Huddinge, Sweden.Show MeSH
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Mentions: The expression of NKRF was detected only in pyramidal neurons, and was predominantly limited to the nucleolus with weak positivity in the nucleoplasm and perinuclear membrane in the control case (Fig. 3A). Although intense staining was detectable in the nucleolus in the AD case, the perinuclear and nucleoplasm staining was weaker than in the controls (Fig. 3B). The expression level in CA1 pyramidal neurons was clearly higher than in pyramidal neurons in CA3 and cortex (data not shown).
Affiliation: Karolinska Institutet and Dainippon Sumitomo Pharma Alzheimer Center, Department of Neurobiology, Care Sciences and Society, NVS, Karolinska Institutet, Huddinge, Sweden.