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Restored perfusion and reduced inflammation in the infarcted heart after grafting stem cells with a hyaluronan-based scaffold.

Muscari C, Bonafè F, Martin-Suarez S, Valgimigli S, Valente S, Fiumana E, Fiorelli F, Rubini G, Guarnieri C, Caldarera CM, Capitani O, Arpesella G, Pasquinelli G - J. Cell. Mol. Med. (2013)

Bottom Line: Apart from the inflammatory response, the native tissue positively interacted with the HYAFF(®) 11/MSCs construct modifying the extracellular matrix with a reduced presence of collagene and increased amount of proteoglycans.The border-zone cardiomyocytes also reacted favourably to the graft as a lower degree of cellular damage was found.These beneficial effects are superior to those obtained after grafting only the scaffold or MSCs, suggesting that a synergic action was achieved using the cell-integrated polymer construct.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Bologna, Bologna 40126, Italy. claudio.muscari@unibo.it

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Related in: MedlinePlus

Vascularization of the infarct area. (a) The blood vessels were identified in the infarct region as circular/oval structures stained with vWF as indicated by the arrows (upper bars, 50 μm; lower bars, 25 μm). (b) The quantitative analysis was performed with all the representative regions of the infarct area (both centre region and border zone). In the bar graph, the number of total vessels corresponds to the sum of capillaries and medium-sized vessels. The number of medium-sized vessels significantly increased in the HYAFF®11 group with respect to the MSCs group (P < 0.05). Ctr infarcted, n = 5; HYAFF®11, n = 3; MSCs, n = 3; HYAFF®11/MSCs, n = 4.
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fig06: Vascularization of the infarct area. (a) The blood vessels were identified in the infarct region as circular/oval structures stained with vWF as indicated by the arrows (upper bars, 50 μm; lower bars, 25 μm). (b) The quantitative analysis was performed with all the representative regions of the infarct area (both centre region and border zone). In the bar graph, the number of total vessels corresponds to the sum of capillaries and medium-sized vessels. The number of medium-sized vessels significantly increased in the HYAFF®11 group with respect to the MSCs group (P < 0.05). Ctr infarcted, n = 5; HYAFF®11, n = 3; MSCs, n = 3; HYAFF®11/MSCs, n = 4.

Mentions: Blood vessels were identified in the infarct region as circular/oval structures lined by one or more vWF+ cells (Fig. 6a). The number of medium-sized vessels was significantly increased in the HYAFF®11 group relative to the MSCs group (P < 0.05; Fig. 6b). The number of capillaries and total vessels (capillaries + medium-sized vessels) was apparently increased in both groups treated with HYAFF-11, but this number did not reach statistical significance with respect to the untreated infarct group.


Restored perfusion and reduced inflammation in the infarcted heart after grafting stem cells with a hyaluronan-based scaffold.

Muscari C, Bonafè F, Martin-Suarez S, Valgimigli S, Valente S, Fiumana E, Fiorelli F, Rubini G, Guarnieri C, Caldarera CM, Capitani O, Arpesella G, Pasquinelli G - J. Cell. Mol. Med. (2013)

Vascularization of the infarct area. (a) The blood vessels were identified in the infarct region as circular/oval structures stained with vWF as indicated by the arrows (upper bars, 50 μm; lower bars, 25 μm). (b) The quantitative analysis was performed with all the representative regions of the infarct area (both centre region and border zone). In the bar graph, the number of total vessels corresponds to the sum of capillaries and medium-sized vessels. The number of medium-sized vessels significantly increased in the HYAFF®11 group with respect to the MSCs group (P < 0.05). Ctr infarcted, n = 5; HYAFF®11, n = 3; MSCs, n = 3; HYAFF®11/MSCs, n = 4.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3822652&req=5

fig06: Vascularization of the infarct area. (a) The blood vessels were identified in the infarct region as circular/oval structures stained with vWF as indicated by the arrows (upper bars, 50 μm; lower bars, 25 μm). (b) The quantitative analysis was performed with all the representative regions of the infarct area (both centre region and border zone). In the bar graph, the number of total vessels corresponds to the sum of capillaries and medium-sized vessels. The number of medium-sized vessels significantly increased in the HYAFF®11 group with respect to the MSCs group (P < 0.05). Ctr infarcted, n = 5; HYAFF®11, n = 3; MSCs, n = 3; HYAFF®11/MSCs, n = 4.
Mentions: Blood vessels were identified in the infarct region as circular/oval structures lined by one or more vWF+ cells (Fig. 6a). The number of medium-sized vessels was significantly increased in the HYAFF®11 group relative to the MSCs group (P < 0.05; Fig. 6b). The number of capillaries and total vessels (capillaries + medium-sized vessels) was apparently increased in both groups treated with HYAFF-11, but this number did not reach statistical significance with respect to the untreated infarct group.

Bottom Line: Apart from the inflammatory response, the native tissue positively interacted with the HYAFF(®) 11/MSCs construct modifying the extracellular matrix with a reduced presence of collagene and increased amount of proteoglycans.The border-zone cardiomyocytes also reacted favourably to the graft as a lower degree of cellular damage was found.These beneficial effects are superior to those obtained after grafting only the scaffold or MSCs, suggesting that a synergic action was achieved using the cell-integrated polymer construct.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemistry, University of Bologna, Bologna 40126, Italy. claudio.muscari@unibo.it

Show MeSH
Related in: MedlinePlus