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Evidence for progressive reduction and loss of telocytes in the dermal cellular network of systemic sclerosis.

Manetti M, Guiducci S, Ruffo M, Rosa I, Faussone-Pellegrini MS, Matucci-Cerinic M, Ibba-Manneschi L - J. Cell. Mol. Med. (2013)

Bottom Line: We presently investigated telocyte distribution and features in the skin of SSc patients compared with normal skin.Telocyte damage and loss evolved differently according to SSc subsets and stages, being more rapid and severe in diffuse SSc.Briefly, in human skin telocytes are a distinct stromal cell population.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Histology and Forensic Medicine, University of Florence, I-50134 Florence, Italy. mirkomanetti@yahoo.it

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Diffuse cutaneous systemic sclerosis (dcSSc) skin, transmission electron microscopy. (A) Early dcSSc. A telocyte with a small perinuclear cytoplasm and slender telopodes is embedded in a matrix composed of closely packed collagen bundles. Swollen mitochondria and vacuoles (arrows) are present in the cytoplasm. (B) Advanced dcSSc. Telocytes and telopodes embedded in the fibrotic extracellular matrix show features of degenerating cells. The cytoplasm is dark and contains swollen mitochondria (arrows), vacuoles and lipofuscinic bodies. Many cell debris are evident. (C) Early dcSSc. A telocyte displaying a normal morphology embraces with telopodes a large and abnormal elastin fibre. (D) Early dcSSc. Normal telocytes surround the thickened basal lamina (asterisk) of a blood vessel displaying a patent lumen. (E) Early dcSSc. Telocytes with a normal morphology are evident around nerve bundles. Abundant collagen fibres separate telopodes from the nerve bundle. A fibroblast is in the close vicinity of a telocyte and is surrounded by telopodes. (F) Advanced dcSSc. Telocytes are not identifiable around an occluded microvessel. Only a few cell debris are observed. The vessel basal lamina is markedly thickened (asterisk). TC: telocyte; Tp: telopode; Coll: collagen; Ela: elastin; E: endothelial cell; P: pericyte; N: nerve; F: fibroblast. Scale bars are indicated in each panel.
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fig08: Diffuse cutaneous systemic sclerosis (dcSSc) skin, transmission electron microscopy. (A) Early dcSSc. A telocyte with a small perinuclear cytoplasm and slender telopodes is embedded in a matrix composed of closely packed collagen bundles. Swollen mitochondria and vacuoles (arrows) are present in the cytoplasm. (B) Advanced dcSSc. Telocytes and telopodes embedded in the fibrotic extracellular matrix show features of degenerating cells. The cytoplasm is dark and contains swollen mitochondria (arrows), vacuoles and lipofuscinic bodies. Many cell debris are evident. (C) Early dcSSc. A telocyte displaying a normal morphology embraces with telopodes a large and abnormal elastin fibre. (D) Early dcSSc. Normal telocytes surround the thickened basal lamina (asterisk) of a blood vessel displaying a patent lumen. (E) Early dcSSc. Telocytes with a normal morphology are evident around nerve bundles. Abundant collagen fibres separate telopodes from the nerve bundle. A fibroblast is in the close vicinity of a telocyte and is surrounded by telopodes. (F) Advanced dcSSc. Telocytes are not identifiable around an occluded microvessel. Only a few cell debris are observed. The vessel basal lamina is markedly thickened (asterisk). TC: telocyte; Tp: telopode; Coll: collagen; Ela: elastin; E: endothelial cell; P: pericyte; N: nerve; F: fibroblast. Scale bars are indicated in each panel.

Mentions: Telocytes were severely reduced in the dermis of SSc patients, and most of them exhibited ultrastructural abnormalities (Figs 7 and 8). However, a few normal telocytes were also present in the majority of SSc patients (Figs 7 and 8). Fibroblasts, myofibroblasts, mast cells and macrophages were present, but only a few fibroblasts showed ultrastructural changes, in particular in advanced dcSSc patients.


Evidence for progressive reduction and loss of telocytes in the dermal cellular network of systemic sclerosis.

Manetti M, Guiducci S, Ruffo M, Rosa I, Faussone-Pellegrini MS, Matucci-Cerinic M, Ibba-Manneschi L - J. Cell. Mol. Med. (2013)

Diffuse cutaneous systemic sclerosis (dcSSc) skin, transmission electron microscopy. (A) Early dcSSc. A telocyte with a small perinuclear cytoplasm and slender telopodes is embedded in a matrix composed of closely packed collagen bundles. Swollen mitochondria and vacuoles (arrows) are present in the cytoplasm. (B) Advanced dcSSc. Telocytes and telopodes embedded in the fibrotic extracellular matrix show features of degenerating cells. The cytoplasm is dark and contains swollen mitochondria (arrows), vacuoles and lipofuscinic bodies. Many cell debris are evident. (C) Early dcSSc. A telocyte displaying a normal morphology embraces with telopodes a large and abnormal elastin fibre. (D) Early dcSSc. Normal telocytes surround the thickened basal lamina (asterisk) of a blood vessel displaying a patent lumen. (E) Early dcSSc. Telocytes with a normal morphology are evident around nerve bundles. Abundant collagen fibres separate telopodes from the nerve bundle. A fibroblast is in the close vicinity of a telocyte and is surrounded by telopodes. (F) Advanced dcSSc. Telocytes are not identifiable around an occluded microvessel. Only a few cell debris are observed. The vessel basal lamina is markedly thickened (asterisk). TC: telocyte; Tp: telopode; Coll: collagen; Ela: elastin; E: endothelial cell; P: pericyte; N: nerve; F: fibroblast. Scale bars are indicated in each panel.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC3822649&req=5

fig08: Diffuse cutaneous systemic sclerosis (dcSSc) skin, transmission electron microscopy. (A) Early dcSSc. A telocyte with a small perinuclear cytoplasm and slender telopodes is embedded in a matrix composed of closely packed collagen bundles. Swollen mitochondria and vacuoles (arrows) are present in the cytoplasm. (B) Advanced dcSSc. Telocytes and telopodes embedded in the fibrotic extracellular matrix show features of degenerating cells. The cytoplasm is dark and contains swollen mitochondria (arrows), vacuoles and lipofuscinic bodies. Many cell debris are evident. (C) Early dcSSc. A telocyte displaying a normal morphology embraces with telopodes a large and abnormal elastin fibre. (D) Early dcSSc. Normal telocytes surround the thickened basal lamina (asterisk) of a blood vessel displaying a patent lumen. (E) Early dcSSc. Telocytes with a normal morphology are evident around nerve bundles. Abundant collagen fibres separate telopodes from the nerve bundle. A fibroblast is in the close vicinity of a telocyte and is surrounded by telopodes. (F) Advanced dcSSc. Telocytes are not identifiable around an occluded microvessel. Only a few cell debris are observed. The vessel basal lamina is markedly thickened (asterisk). TC: telocyte; Tp: telopode; Coll: collagen; Ela: elastin; E: endothelial cell; P: pericyte; N: nerve; F: fibroblast. Scale bars are indicated in each panel.
Mentions: Telocytes were severely reduced in the dermis of SSc patients, and most of them exhibited ultrastructural abnormalities (Figs 7 and 8). However, a few normal telocytes were also present in the majority of SSc patients (Figs 7 and 8). Fibroblasts, myofibroblasts, mast cells and macrophages were present, but only a few fibroblasts showed ultrastructural changes, in particular in advanced dcSSc patients.

Bottom Line: We presently investigated telocyte distribution and features in the skin of SSc patients compared with normal skin.Telocyte damage and loss evolved differently according to SSc subsets and stages, being more rapid and severe in diffuse SSc.Briefly, in human skin telocytes are a distinct stromal cell population.

View Article: PubMed Central - PubMed

Affiliation: Department of Anatomy, Histology and Forensic Medicine, University of Florence, I-50134 Florence, Italy. mirkomanetti@yahoo.it

Show MeSH
Related in: MedlinePlus